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--- nikon_htm-hcs [2025/01/09 15:28]
bioimaging [Objectives]
+++ nikon_htm-hcs [2025/03/28 17:07] (current)
bioimaging [Microscope overview]
@@ Line 28: / Line 28: @@
 ===== Microscope overview =====
-The Nikon-HTM/HCS microscope is a fully automated microscope capable of high-throughput imaging and screening of samples (eg, Ibidi chamber slides or well-plates); with the help of fully programmable and customizable acquisition protocols.
+The Nikon HTM-HCS is a fully automated microscope capable of high-throughput imaging and screening of samples (eg, Ibidi chamber slides or well-plates); with the help of fully programmable and customizable acquisition protocols.
 The system is equipped with a CoolLED fluorescence illuminator with 4 excitation bands, and an Andor Zyla 4.2 sCMOS camera with 4.0 MPixels with a large field of view capable of maximum of 100 FPS at full frame (2048x2048).
@@ Line 34: / Line 34: @@
 The optics available include fluorescence filters and brightfield, including DIC (for several magnifications) and phase-contrast (40x LWD only).
-===== System components =====
+===== LED lines =====
+This system is equipped with a CoolLED fluorescence illuminator with 4 excitation lines:
+  * 380 nm - E.g. DAPI
+  * 470 nm - E.g. FITC
+  * 550 nm - E.g. TRITC
+  * 635 nm - E.g. Cy5
+===== Filterset cubes =====
 ^  Position  ^  Filterset  ^  Reference  ^  Excitation  ^  Dichroic  ^  Emission  ^
-|    |    |    |    |    |    |
+|  1  |  Quad-DAPI  |    |  378/58  |  [[https://www.idex-hs.com/store/product-detail/ff409_493_573_652_di02_25x36/fl-007110?search=true|409]]  |  432/40  |
+|  1  |  Quad-FITC  |    |  474/30  |  [[https://www.idex-hs.com/store/product-detail/ff409_493_573_652_di02_25x36/fl-007110?search=true|493]]  |  515/36  |
+|  1  |  Quad-TRITC  |    |  554/26  |  [[https://www.idex-hs.com/store/product-detail/ff409_493_573_652_di02_25x36/fl-007110?search=true|573]]  |  595/36  |
+|  1  |  Quad-Cy5  |    |  635/22  |  [[https://www.idex-hs.com/store/product-detail/ff409_493_573_652_di02_25x36/fl-007110?search=true|652]]  |  LP 658  |
+|  2  |  FITC  |    |  474/27  |  495  |  525/45  |
+|  3  |  TRITC  |    |  543/22  |  562  |  593/40  |
+|  4  |  Cy5  |    |  635/18  |  652  |  680/42  |
+===== Fast Filter Wheel emission filters =====
-==== Objectives ====
+^  Filterset  ^  Reference  ^  Transmission  ^
-^  Magnification  ^  Model  ^  Immersion  ^  NA  ^  WD (mm)  ^  PFS compatible  ^  DIC compatible  ^  PhC compatible  ^  Reference  ^
+|  DAPI-FW  |  [[https://www.chroma.com/products/parts/at460-50m|at460-50m]]  |  460/50  |
-|  10x  |  Plan Apo  |  Air  |  0.45  |  4  |  No  |  Yes (N1)  |  No  |  [[https://www.microscope.healthcare.nikon.com/en_EU/products/optics/selector/comparison/-179798|MRD70170]]  |
+|  FITC-FW  |  [[https://www.chroma.com/products/parts/et525-50m|et525-50m]]  |  525/50  |
-|  20x  |  Plan Apo Lambda  |  Air  |  0.75  |  1  |  Yes  |  Yes (N1)  |  No  |  MRD00205*  |
+|  TRITC-FW  |  [[https://www.chroma.com/products/parts/et595-50m|et595-50m]]  |  595/50  |
-|  100x  |  Plan Apo Lambda  |  Oil  |  1.45  |  0.13  |  Yes  |  Yes (N2)  |  No  |  MRD01905*  |
+|  Cy5-FW  |  [[https://www.chroma.com/products/parts/s685-40m|s685-40m]]  |  685/40  |
-|  40x  |  Plan Fluor  |  Oil  |  1.3  |  0.24  |  Yes  |  Yes (N2)  |  No  |  [[https://www.microscope.healthcare.nikon.com/en_EU/products/optics/selector/comparison/-1829|MRH01401]]  |
+===== Objectives =====
-|  40x  |  Plan Apo  |  Air  |  0.95  |  0.17-0.25  |  Yes  |  Yes (N2)  |  No  |  MRD00400*  |
+^  Magnification  ^  Model  ^  Immersion  ^  NA  ^  WD (mm)  ^  PFS  ^  DIC  ^  PhC  ^  Reference  ^  Pixel size (um) (1x zoom)  ^  Pixel size (um) (1.5x zoom)  ^
-|  40x  |  LWD* *  |  Air  |  0.55  |  2.1  |  No  |  No  |  Yes  |  [[https://www.microscope.healthcare.nikon.com/en_EU/products/optics/selector/comparison/-1683|MRP46402]]  |
+|  10x  |  Plan Apo  |  Air  |  0.45  |  4  |  No  |  Yes (N1)  |  No  |  [[https://www.microscope.healthcare.nikon.com/en_EU/products/optics/selector/comparison/-179798|MRD70170]]  |  0.643  |  0.429  |
+|  20x  |  Plan Apo Lambda  |  Air  |  0.75  |  1  |  Yes  |  Yes (N1)  |  No  |  MRD00205*  |  0.323  |  0.216  |
+|  100x  |  Plan Apo Lambda  |  Oil  |  1.45  |  0.13  |  Yes  |  Yes (N2)  |  No  |  MRD01905*  |  0.065  |  0.043  |
+|  40x  |  Plan Fluor  |  Oil  |  1.3  |  0.24  |  Yes  |  Yes (N2)  |  No  |  [[https://www.microscope.healthcare.nikon.com/en_EU/products/optics/selector/comparison/-1829|MRH01401]]  |  0.162  |  0.108  |
+|  40x  |  Plan Apo  |  Air  |  0.95  |  0.17-0.25  |  Yes  |  Yes (N2)  |  No  |  MRD00400*  |  0.161  |  0.107  |
+|  40x  |  LWD* *  |  Air  |  0.55  |  2.1  |  No  |  No  |  Yes  |  [[https://www.microscope.healthcare.nikon.com/en_EU/products/optics/selector/comparison/-1683|MRP46402]]  |  0.161  |  0.108  |
 * Not fabricated anymore ; * * LWD - Long Working Distance
-==== Camera ====
+The Bioimaging Platform **highly advises against using the 40x LWD objective with 1.5x zoom**
+===== Camera =====
+^  Model  ^  Frame Size  ^  Pixel Size (µm)  ^  Quantum Efficiency  ^
+|  [[https://andor.oxinst.cn/assets/uploads/products/andor/documents/Zyla-4.2-Specifications-Life-Science.pdf|Andor Zyla 4.2 PLUS]]  |  2048 × 2048  |  6.5 x 6.5  |  82%  |
+===== Microscope Turn On Procedure =====
+  * Turn on the CoolLed controller on **top of the microscope's shelf**.
+{{nikon-htm_turnon_1.png?200|}}
+  * Turn on the microscope using the **switch** on the **bottom of the microscope's optical table**.
+{{nikon-htm_turnon_2.png?200|}}
+===== Microscope Turn Off Procedure =====
+  * Turn off the microscope using the **switch** on the **bottom of the microscope's optical table**.
+{{nikon-htm_turnon_2.png?200|}}
+  * Turn off the CoolLed controller on **top of the microscope's shelf**.
+{{nikon-htm_turnon_1.png?200|}}
 ----
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