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nikon_htm-hcs [2026/02/19 18:45] bioimaging [Microscope Turn Off Procedure] |
nikon_htm-hcs [2026/05/26 11:43] (current) bioimaging |
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| The Nikon HTM-HCS is a fully automated microscope capable of high-throughput imaging and screening of samples (eg, Ibidi chamber slides or well-plates); with the help of fully programmable and customizable acquisition protocols. | The Nikon HTM-HCS is a fully automated microscope capable of high-throughput imaging and screening of samples (eg, Ibidi chamber slides or well-plates); with the help of fully programmable and customizable acquisition protocols. | ||
| - | The system is equipped with a CoolLED fluorescence illuminator with 4 excitation bands, and an Andor Zyla 4.2 sCMOS camera with 4.0 MPixels with a large field of view capable of maximum of 100 FPS at full frame (2048x2048). | + | The system is equipped with a CoolLED fluorescence illuminator with 4 excitation bands, and an [[https://www.biovis.com/images/cameras/SCAS0122E_C11440-42U30.pdf|ORCA-Flash4.0 LT+ sCMOS camera]] with 4.0 MPixels (2048x2048) with a large field of view, low-light sensitivity, and large dynamic range. |
| The optics available include fluorescence filters and brightfield, including DIC (for several magnifications) and phase-contrast (40x LWD only). | The optics available include fluorescence filters and brightfield, including DIC (for several magnifications) and phase-contrast (40x LWD only). | ||
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| ^ Model ^ Frame Size ^ Pixel Size (µm) ^ Quantum Efficiency ^ | ^ Model ^ Frame Size ^ Pixel Size (µm) ^ Quantum Efficiency ^ | ||
| - | | [[https://andor.oxinst.cn/assets/uploads/products/andor/documents/Zyla-4.2-Specifications-Life-Science.pdf|Andor Zyla 4.2 PLUS]] | 2048 × 2048 | 6.5 x 6.5 | 82% | | + | | [[https://www.biovis.com/images/cameras/SCAS0122E_C11440-42U30.pdf|ORCA-Flash4.0 LT PLUS]] | 2048 × 2048 | 6.5 x 6.5 | 82% | |
| ===== Microscope Turn On Procedure ===== | ===== Microscope Turn On Procedure ===== | ||
nikon_htm-hcs.txt · Last modified: 2026/05/26 11:43 by bioimaging
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