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leica_stellaris_sted_flim [2026/09/04 18:50] bioimaging |
leica_stellaris_sted_flim [2026/09/10 15:31] (current) bioimaging [LASERs] |
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| {{ :sted_principle.png?240}} The Leica STELLARIS STED FLIM is a [[https://www.youtube.com/watch?v=rTkjQ6Q0p5I|point scanning confocal microscope]] designed for advanced fluorescence imaging, [[https://www.leica-microsystems.com/science-lab/life-science/a-guide-to-fluorescence-lifetime-imaging-microscopy-flim/|fluorescence lifetime imaging (FLIM)]] and super-resolution microscopy. Built on the [[https://www.leica-microsystems.com/products/confocal-microscopes/p/leica-stellaris/|Leica STELLARIS platform]], it features a [[https://www.leica-microsystems.com/science-lab/life-science/stellaris-white-light-lasers/|White Light LASER]] (440-790 nm) and 405 nm laser as excitation sources, AOBS beam splitting, highly sensitive [[https://www.leica-microsystems.com/science-lab/life-science/power-hyd-detectors/|Power HyD detectors]] and spectral detection for flexible multicolor imaging, including near-infrared fluorophores up to 850 nm, such as Cy7. The system is optimized for live-cell imaging, featuring a motorized stage, SuperZ Galvo Stage for rapid Z acquisition, hardware autofocus (AFC) and a temperature- and CO₂-controlled Okolab dark enclosure for live-cell imaging in physiological conditions. FLIM capabilities are provided by the Power HyD X detector and [[https://www.leica-microsystems.com/products/confocal-microscopes/p/stellaris-8-falcon/|STELLARIS FALCON (FAst Lifetime CONtrast)]], enabling fast lifetime imaging, [[https://www.leica-microsystems.com/science-lab/life-science/what-is-fret-with-flim/|FLIM-FRET experiments]], [[https://www.leica-microsystems.com/science-lab/life-science/phasor-analysis-for-flim-fluorescence-lifetime-imaging-microscopy/|phasor analysis]] and [[https://www.leica-microsystems.com/science-lab/life-science/tausense-technology-imaging-tools/|TauSense]] lifetime-based contrast and multiplexing tools. | {{ :sted_principle.png?240}} The Leica STELLARIS STED FLIM is a [[https://www.youtube.com/watch?v=rTkjQ6Q0p5I|point scanning confocal microscope]] designed for advanced fluorescence imaging, [[https://www.leica-microsystems.com/science-lab/life-science/a-guide-to-fluorescence-lifetime-imaging-microscopy-flim/|fluorescence lifetime imaging (FLIM)]] and super-resolution microscopy. Built on the [[https://www.leica-microsystems.com/products/confocal-microscopes/p/leica-stellaris/|Leica STELLARIS platform]], it features a [[https://www.leica-microsystems.com/science-lab/life-science/stellaris-white-light-lasers/|White Light LASER]] (440-790 nm) and 405 nm laser as excitation sources, AOBS beam splitting, highly sensitive [[https://www.leica-microsystems.com/science-lab/life-science/power-hyd-detectors/|Power HyD detectors]] and spectral detection for flexible multicolor imaging, including near-infrared fluorophores up to 850 nm, such as Cy7. The system is optimized for live-cell imaging, featuring a motorized stage, SuperZ Galvo Stage for rapid Z acquisition, hardware autofocus (AFC) and a temperature- and CO₂-controlled Okolab dark enclosure for live-cell imaging in physiological conditions. FLIM capabilities are provided by the Power HyD X detector and [[https://www.leica-microsystems.com/products/confocal-microscopes/p/stellaris-8-falcon/|STELLARIS FALCON (FAst Lifetime CONtrast)]], enabling fast lifetime imaging, [[https://www.leica-microsystems.com/science-lab/life-science/what-is-fret-with-flim/|FLIM-FRET experiments]], [[https://www.leica-microsystems.com/science-lab/life-science/phasor-analysis-for-flim-fluorescence-lifetime-imaging-microscopy/|phasor analysis]] and [[https://www.leica-microsystems.com/science-lab/life-science/tausense-technology-imaging-tools/|TauSense]] lifetime-based contrast and multiplexing tools. | ||
| - | For super-resolution imaging, the system is equipped with 3D STED technology using pulsed 589 nm and 775 nm depletion lasers, providing resolution well below the diffraction limit. [[https://www.leica-microsystems.com/science-lab/life-science/extended-live-cell-imaging-at-nanoscale-resolution/|TauSTED Xtend]] combines STED and lifetime information to improve image quality and resolution while reducing light exposure, making the system particularly suitable for live-cell nanoscale imaging. | + | For super-resolution imaging, the system is equipped with [[https://www.leica-microsystems.com/science-lab/life-science/a-guide-to-super-resolution/|2D and 3D STED technology]] using pulsed 589 nm and 775 nm depletion lasers, providing resolution well below the diffraction limit. [[https://www.leica-microsystems.com/science-lab/life-science/extended-live-cell-imaging-at-nanoscale-resolution/|TauSTED Xtend]] combines STED and lifetime information to improve image quality and resolution while reducing light exposure, making the system particularly suitable for live-cell nanoscale imaging. |
| * **Microscope**: [[https://www.leica-microsystems.com/products/light-microscopes/p/dmi8/|Leica DMi8 CS inverted]] | * **Microscope**: [[https://www.leica-microsystems.com/products/light-microscopes/p/dmi8/|Leica DMi8 CS inverted]] | ||
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| ^ LASER ^ Wavelength ^ | ^ LASER ^ Wavelength ^ | ||
| | Violet | <fc #a600ff>405 nm</fc> | | | Violet | <fc #a600ff>405 nm</fc> | | ||
| - | | WLL | <fc #0000ff>440 nm</fc> - <fc #be0000>790 nm</fc> | | + | | WLL | <fc #0071ff>440 nm</fc> - <fc #be0000>790 nm</fc> | |
| ==== Filterset (for eyepieces) ==== | ==== Filterset (for eyepieces) ==== | ||
leica_stellaris_sted_flim.1788540613.txt.gz · Last modified: 2026/09/04 18:50 by bioimaging
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