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leica_stellaris_sted_flim
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leica_stellaris_sted_flim [2026/09/04 01:59] bioimaging |
leica_stellaris_sted_flim [2026/09/10 15:31] (current) bioimaging [LASERs] |
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| ===== Microscope overview ===== | ===== Microscope overview ===== | ||
| - | {{ :sted_principle.png?250}} The Leica STELLARIS STED FLIM is a [[https://www.youtube.com/watch?v=rTkjQ6Q0p5I|point scanning confocal microscope]] designed for advanced fluorescence imaging, super-resolution microscopy and fluorescence lifetime measurements. Built on the [[https://www.leica-microsystems.com/products/confocal-microscopes/p/leica-stellaris/|Leica STELLARIS platform]], it combines a [[https://www.leica-microsystems.com/science-lab/life-science/stellaris-white-light-lasers/|White Light LASER]] (WLL) excitation source, an Acousto-Optical Beam Splitter (AOBS), highly sensitive [[https://www.leica-microsystems.com/science-lab/life-science/power-hyd-detectors/|Power HyD detectors]] and prism-based spectral detection to provide flexible excitation of up to 8 simultaneous laser lines from 440 to 790 nm. When combined with the Power HyD R detector, you can detect fluorophores in the near infrared range (up to 850 nm) such as Cy7. It is also equipped with a 405 nm diode laser, a motorized XY scanning stage, a SuperZ Galvo Stage for rapid Z acquisition, and an Adaptive Focus Control (AFC) closed-loop hardware autofocus system for long-term stability during time-lapse experiments. A large environmental dark Okolab enclosure with temperature and CO2 control enables live-cell imaging under physiological conditions. The confocal scanhead incorporates a field-of-view scanner with galvanometric mirrors optimized for high resolution and sensitivity and includes a Power HyD X detector optimized for [[https://www.leica-microsystems.com/science-lab/life-science/a-guide-to-fluorescence-lifetime-imaging-microscopy-flim/|fluorescence lifetime imaging (FLIM)]]. FLIM measures the lifetime of fluorophores, a photophysical property that is independent of fluorescence intensity and can be used to perform Förster Resonance Energy Transfer (FRET) experiments, enabling quantitative studies of molecular interactions, environmental sensing and dynamic cellular processes. The system includes [[https://www.leica-microsystems.com/products/confocal-microscopes/p/stellaris-8-falcon/|STELLARIS FALCON (FAst Lifetime CONtrast)]], enabling FLIM imaging at confocal speeds and supporting exponential fitting, [[https://www.leica-microsystems.com/science-lab/life-science/phasor-analysis-for-flim-fluorescence-lifetime-imaging-microscopy/|FLIM phasor analysis]], pattern fitting and [[https://www.leica-microsystems.com/science-lab/life-science/what-is-fret-with-flim/|FLIM-FRET analysis]]. The [[https://www.leica-microsystems.com/science-lab/life-science/tausense-technology-imaging-tools/|TauSense]] imaging tools include TauContrast, which uses fluorescence lifetime to provide contrast that is independent from fluorescent intensity, TauGating which uses differences in average photon arrival time to remove unwanted background and TauSeparation for multiplexing using differences in fluorescence lifetime. For super-resolution imaging, the system is equipped with STED technology, including pulsed 589 nm and 775 nm depletion lasers, enabling imaging with resolution well below the diffraction limit. The 3D STED module provides tunable super-resolution in X, Y and Z, while [[https://www.leica-microsystems.com/science-lab/life-science/extended-live-cell-imaging-at-nanoscale-resolution/|TauSTED Xtend]] combines STED and fluorescence lifetime information to improve image quality, contrast and resolution at lower light doses, making it particularly suitable for live-cell super-resolution imaging. The system supports multicolour STED imaging across much of the visible spectrum and facilitates the investigation of molecular organization, interactions and nanoscale cellular structures. | + | {{ :sted_principle.png?240}} The Leica STELLARIS STED FLIM is a [[https://www.youtube.com/watch?v=rTkjQ6Q0p5I|point scanning confocal microscope]] designed for advanced fluorescence imaging, [[https://www.leica-microsystems.com/science-lab/life-science/a-guide-to-fluorescence-lifetime-imaging-microscopy-flim/|fluorescence lifetime imaging (FLIM)]] and super-resolution microscopy. Built on the [[https://www.leica-microsystems.com/products/confocal-microscopes/p/leica-stellaris/|Leica STELLARIS platform]], it features a [[https://www.leica-microsystems.com/science-lab/life-science/stellaris-white-light-lasers/|White Light LASER]] (440-790 nm) and 405 nm laser as excitation sources, AOBS beam splitting, highly sensitive [[https://www.leica-microsystems.com/science-lab/life-science/power-hyd-detectors/|Power HyD detectors]] and spectral detection for flexible multicolor imaging, including near-infrared fluorophores up to 850 nm, such as Cy7. The system is optimized for live-cell imaging, featuring a motorized stage, SuperZ Galvo Stage for rapid Z acquisition, hardware autofocus (AFC) and a temperature- and CO₂-controlled Okolab dark enclosure for live-cell imaging in physiological conditions. FLIM capabilities are provided by the Power HyD X detector and [[https://www.leica-microsystems.com/products/confocal-microscopes/p/stellaris-8-falcon/|STELLARIS FALCON (FAst Lifetime CONtrast)]], enabling fast lifetime imaging, [[https://www.leica-microsystems.com/science-lab/life-science/what-is-fret-with-flim/|FLIM-FRET experiments]], [[https://www.leica-microsystems.com/science-lab/life-science/phasor-analysis-for-flim-fluorescence-lifetime-imaging-microscopy/|phasor analysis]] and [[https://www.leica-microsystems.com/science-lab/life-science/tausense-technology-imaging-tools/|TauSense]] lifetime-based contrast and multiplexing tools. |
| + | For super-resolution imaging, the system is equipped with [[https://www.leica-microsystems.com/science-lab/life-science/a-guide-to-super-resolution/|2D and 3D STED technology]] using pulsed 589 nm and 775 nm depletion lasers, providing resolution well below the diffraction limit. [[https://www.leica-microsystems.com/science-lab/life-science/extended-live-cell-imaging-at-nanoscale-resolution/|TauSTED Xtend]] combines STED and lifetime information to improve image quality and resolution while reducing light exposure, making the system particularly suitable for live-cell nanoscale imaging. | ||
| * **Microscope**: [[https://www.leica-microsystems.com/products/light-microscopes/p/dmi8/|Leica DMi8 CS inverted]] | * **Microscope**: [[https://www.leica-microsystems.com/products/light-microscopes/p/dmi8/|Leica DMi8 CS inverted]] | ||
| * **LED Illumination**: [[https://www.coolled.com/products/pe-300white/|CoolLED pE-300white]] | * **LED Illumination**: [[https://www.coolled.com/products/pe-300white/|CoolLED pE-300white]] | ||
| - | * **Detectors**: [[https://www.leica-microsystems.com/science-lab/life-science/power-hyd-detectors/|Power hybrid detectors]] (Power HyD S, Power HyD X, and Power HyD R) | + | * **Detectors**: [[https://www.leica-microsystems.com/science-lab/life-science/power-hyd-detectors/|Power hybrid detectors]] |
| + | * Power HyD S | ||
| + | * Power HyD X for FLIM imaging | ||
| + | * Power HyD R for near-infrared imaging | ||
| * **Features** | * **Features** | ||
| * | [[https://www.leica-microsystems.com/science-lab/life-science/a-guide-to-super-resolution/|2D and 3D Super Resolution STED]] | * | [[https://www.leica-microsystems.com/science-lab/life-science/a-guide-to-super-resolution/|2D and 3D Super Resolution STED]] | ||
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| ^ LASER ^ Wavelength ^ | ^ LASER ^ Wavelength ^ | ||
| | Violet | <fc #a600ff>405 nm</fc> | | | Violet | <fc #a600ff>405 nm</fc> | | ||
| - | | WLL | <fc #0000ff>440 nm</fc> - <fc #be0000>790 nm</fc> | | + | | WLL | <fc #0071ff>440 nm</fc> - <fc #be0000>790 nm</fc> | |
| ==== Filterset (for eyepieces) ==== | ==== Filterset (for eyepieces) ==== | ||
leica_stellaris_sted_flim.1788479957.txt.gz · Last modified: 2026/09/04 01:59 by bioimaging
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