Differences

This shows you the differences between two versions of the page.

--- leica_stellaris_sted_flim [2026/09/03 17:52]
bioimaging
+++ leica_stellaris_sted_flim [2026/09/03 18:11] (current)
bioimaging
@@ Line 28: / Line 28: @@
 ===== Microscope overview =====
-The Leica STELLARIS STED FLIM is a [[https://www.youtube.com/watch?v=rTkjQ6Q0p5I|point scanning confocal microscope]] designed for advanced fluorescence imaging, super-resolution microscopy and fluorescence lifetime measurements. Built on the [[https://www.leica-microsystems.com/products/confocal-microscopes/p/leica-stellaris/|Leica STELLARIS platform]], it combines a [[https://www.leica-microsystems.com/science-lab/life-science/stellaris-white-light-lasers/|White Light LASER]] (WLL) excitation source with highly sensitive Power HyD detectors and prism-based spectral detection, providing flexible excitation from 440 to 790 nm and fluorescence detection up to 850 nm. It is also equipped with a 405 nm diode laser. The system is equipped with a motorized XY scanning stage, a SuperZ Galvo Stage for rapid Z acquisition, and an Adaptive Focus Control (AFC) closed-loop hardware autofocus system for long-term stability during time-lapse experiments. A large environmental enclosure with temperature and CO2 control enables live-cell imaging under physiological conditions. The confocal scanhead incorporates a field-of-view scanner with galvanometric mirrors optimized for high resolution and sensitivity. Detection is provided by multiple Power HyD detectors, including Power HyD X detector optimized for fluorescence lifetime applications, fluorescence correlation spectroscopy (FCS), and Tau-STED measurements. For super-resolution imaging, the system is equipped with STED technology, including pulsed 589 nm and 775 nm depletion lasers, enabling imaging with resolution well below the diffraction limit. The 3D STED module provides tunable super-resolution in X, Y and Z, while TauSTED Xtend combines STED and fluorescence lifetime information to improve image quality, contrast and resolution at lower light doses, making it particularly suitable for live-cell super-resolution imaging. The system supports multicolour STED imaging across much of the visible spectrum and facilitates the investigation of molecular organization, interactions and nanoscale cellular structures. The microscope also includes STELLARIS FALCON (FAst Lifetime CONtrast), providing fluorescence lifetime imaging microscopy (FLIM) at confocal imaging speeds. Fully integrated within LAS X software, FALCON supports exponential fitting, FLIM phasor analysis, pattern fitting and FLIM-FRET analysis, enabling quantitative studies of molecular interactions, environmental sensing and dynamic cellular processes.
+The Leica STELLARIS STED FLIM is a [[https://www.youtube.com/watch?v=rTkjQ6Q0p5I|point scanning confocal microscope]] designed for advanced fluorescence imaging, super-resolution microscopy and fluorescence lifetime measurements. Built on the [[https://www.leica-microsystems.com/products/confocal-microscopes/p/leica-stellaris/|Leica STELLARIS platform]], it combines a [[https://www.leica-microsystems.com/science-lab/life-science/stellaris-white-light-lasers/|White Light LASER]] (WLL) excitation source with highly sensitive [[https://www.leica-microsystems.com/science-lab/life-science/power-hyd-detectors/|Power HyD detectors]] and prism-based spectral detection, providing flexible excitation from 440 to 790 nm and fluorescence detection up to 850 nm. It is also equipped with a 405 nm diode laser. The system is equipped with a motorized XY scanning stage, a SuperZ Galvo Stage for rapid Z acquisition, and an Adaptive Focus Control (AFC) closed-loop hardware autofocus system for long-term stability during time-lapse experiments. A large environmental enclosure with temperature and CO2 control enables live-cell imaging under physiological conditions. The confocal scanhead incorporates a field-of-view scanner with galvanometric mirrors optimized for high resolution and sensitivity and includes a Power HyD X detector optimized for [[https://www.leica-microsystems.com/science-lab/life-science/a-guide-to-fluorescence-lifetime-imaging-microscopy-flim/|fluorescence lifetime applications]], fluorescence correlation spectroscopy (FCS), and [[https://www.leica-microsystems.com/products/confocal-microscopes/p/stellaris-sted/|Tau-STED measurements]]. For super-resolution imaging, the system is equipped with STED technology, including pulsed 589 nm and 775 nm depletion lasers, enabling imaging with resolution well below the diffraction limit. The 3D STED module provides tunable super-resolution in X, Y and Z, while [[https://www.leica-microsystems.com/science-lab/life-science/extended-live-cell-imaging-at-nanoscale-resolution/|TauSTED Xtend]] combines STED and fluorescence lifetime information to improve image quality, contrast and resolution at lower light doses, making it particularly suitable for live-cell super-resolution imaging. The system supports multicolour STED imaging across much of the visible spectrum and facilitates the investigation of molecular organization, interactions and nanoscale cellular structures. The microscope also includes [[https://www.leica-microsystems.com/products/confocal-microscopes/p/stellaris-8-falcon/|STELLARIS FALCON (FAst Lifetime CONtrast)]], providing fluorescence lifetime imaging microscopy (FLIM) at confocal imaging speeds. Fully integrated within LAS X software, FALCON supports exponential fitting, [[https://www.leica-microsystems.com/science-lab/life-science/phasor-analysis-for-flim-fluorescence-lifetime-imaging-microscopy/|FLIM phasor analysis]], pattern fitting and [[https://www.leica-microsystems.com/science-lab/life-science/what-is-fret-with-flim/|FLIM-FRET analysis]], enabling quantitative studies of molecular interactions, environmental sensing and dynamic cellular processes.
   * **Microscope**: [[https://www.leica-microsystems.com/products/light-microscopes/p/dmi8/|Leica DMi8 CS inverted]]

GIMM Bioimaging Wiki

User Tools

Site Tools

Differences

Page Tools