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leica_sdc [2026/02/02 01:37] bioimaging [Objectives] |
leica_sdc [2026/02/02 01:46] (current) bioimaging [Emission Filters Spinning Disk] |
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| ==== Emission Filters Spinning Disk ==== | ==== Emission Filters Spinning Disk ==== | ||
| - | ^ Name ^ Emission λ ^ Most common fluorophores ^ | + | ^ Filter name ^ Transmission ^ Most common fluorophores ^ |
| - | | CSU 405 BP | 447/60 | DAPI or Hoechst | | + | | CSU 405 BP | <fc #0035ff>417-477 nm</fc> | DAPI or Hoechst | |
| - | | CSU 488 BP | 525/50 | GFP, FITC or Alexa488 | | + | | CSU 488 BP | <fc #4aff00>500-550 nm</fc> | GFP, FITC or Alexa488 | |
| - | | CSU 561 BP | 607/36 | RFP, TRITC or A568 | | + | | CSU 561 BP | <fc #ffa600>589-625 nm</fc> | RFP, TRITC or A568 | |
| - | | CSU 640 BP | 685/40 | Cy5, A633 or A647 | | + | | CSU 640 BP | <fc #ff0000>665-705 nm</fc> | Cy5, A633 or A647 | |
| | DIC | - | Bright field with DIC optics | | | DIC | - | Bright field with DIC optics | | ||
| - | | Quad | 440/521/607/700 | DAPI+A488+A568/A647 | | + | | Quad | <fc #0041ff>440</fc>+<fc #4aff00>521</fc>+<fc #ffa600>607</fc>+<fc #ff0000>700</fc> nm | DAPI+A488+A568/A647 | |