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leica_sdc [2026/02/02 00:29] bioimaging |
leica_sdc [2026/02/02 01:46] (current) bioimaging [Emission Filters Spinning Disk] |
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| ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Pixel Size (μm/pixel) ^ Reference ^ | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Pixel Size (μm/pixel) ^ Reference ^ | ||
| - | | <fc #fef042>10x</fc> | - | Air | 0.30 | 7.8 | 1.300 | [[https://www.e-leica.com/Objectives.nsf/showObjectivePage?openagent&id=3371AC838F8F6AAAC1257EED002C29F4|Ref]] | | + | | <fc #fef042>10x</fc> | - | Air | 0.30 | 7.8 | 1.300 | - | |
| - | | <fc #0aa05c>20x</fc> | HC PL APO | Air | 0.80 | 0.4 | 0.650 | [[https://www.e-leica.com/Objectives.nsf/showObjectivePage?openagent&id=3371AC838F8F6AAAC1257EED002C29F4|Ref]] | | + | | <fc #0aa05c>20x</fc> | HC PL APO | Air | 0.80 | 0.4 | 0.650 | [[https://www.leica-microsystems.com/objectivefinder/detail/objective/506529/|506529]] | |
| - | | <fc #2c80c0>63x</fc> | PLAN APO | Gly | 1.30 | 0.3 | 0.137 | [[https://www.e-leica.com/Objectives.nsf/showObjectivePage?openagent&id=DE8B34D0C20525C2C1257EED002C29A1|Ref]] | | + | | <fc #2c80c0>63x</fc> | HC PL APO | Gly | 1.30 | 0.3 | 0.137 | [[https://www.leica-microsystems.com/objectivefinder/detail/objective/506398/|506398]] | |
| - | | 100x | PLAN APO | Oil | 1.40 | 0.13 | 0.130 | [[https://www.e-leica.com/Objectives.nsf/showObjectivePage?openagent&id=DB84BAA7AC124EB5C1257EED002C29C3|Ref]] | | + | | 100x | HC PL APO | Oil | 1.40 | 0.13 | 0.130 | [[https://www.leica-microsystems.com/objectivefinder/detail/objective/506372/|506372]] | |
| ==== Emission Filters Spinning Disk ==== | ==== Emission Filters Spinning Disk ==== | ||
| - | ^ Name ^ Emission λ ^ Most common fluorophores ^ | + | ^ Filter name ^ Transmission ^ Most common fluorophores ^ |
| - | | CSU 405 BP | 447/60 | DAPI or Hoechst | | + | | CSU 405 BP | <fc #0035ff>417-477 nm</fc> | DAPI or Hoechst | |
| - | | CSU 488 BP | 525/50 | GFP, FITC or Alexa488 | | + | | CSU 488 BP | <fc #4aff00>500-550 nm</fc> | GFP, FITC or Alexa488 | |
| - | | CSU 561 BP | 607/36 | RFP, TRITC or A568 | | + | | CSU 561 BP | <fc #ffa600>589-625 nm</fc> | RFP, TRITC or A568 | |
| - | | CSU 640 BP | 685/40 | Cy5, A633 or A647 | | + | | CSU 640 BP | <fc #ff0000>665-705 nm</fc> | Cy5, A633 or A647 | |
| | DIC | - | Bright field with DIC optics | | | DIC | - | Bright field with DIC optics | | ||
| - | | Quad | 440/521/607/700 | DAPI+A488+A568/A647 | | + | | Quad | <fc #0041ff>440</fc>+<fc #4aff00>521</fc>+<fc #ffa600>607</fc>+<fc #ff0000>700</fc> nm | DAPI+A488+A568/A647 | |
leica_sdc.1769988595.txt.gz · Last modified: 2026/02/02 00:29 by bioimaging
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