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deltavision
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deltavision [2025/01/11 19:13] bioimaging |
deltavision [2025/12/12 11:03] (current) bioimaging |
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| <a href="/facility/bioimaging/lib/exe/fetch.php?media=deltavision.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=deltavision.jpg" width=500></a> | <a href="/facility/bioimaging/lib/exe/fetch.php?media=deltavision.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=deltavision.jpg" width=500></a> | ||
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| - | <b>Location</b>: [Oeiras] Room 0B02<br> | + | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=bartolomeu_dias_wing_2025_-_deltavision.png">[Oeiras] Room 0B02</a><br> |
| <b>Manufacturer</b>: GE (Currently Leica) <br> | <b>Manufacturer</b>: GE (Currently Leica) <br> | ||
| <b>Model</b>: DeltaVision OMX-SIM <br> | <b>Model</b>: DeltaVision OMX-SIM <br> | ||
| Line 34: | Line 34: | ||
| ==== Lasers ==== | ==== Lasers ==== | ||
| ^ Excitation ^ Wavelength ^ Power ^ | ^ Excitation ^ Wavelength ^ Power ^ | ||
| - | | Violet | 405 nm | 100mW | | + | | Violet | <fc #a600ff>405 nm</fc> | 100mW | |
| - | | Blue | 488 nm | 100mW | | + | | Blue | <fc #00f7ff>488 nm</fc> | 100mW | |
| - | | Green | 568 nm | 100mW | | + | | Yellow-Green | <fc #dbff00>568 nm</fc> | 100mW | |
| - | | Red | 640 nm | 100mW | | + | | Red | <fc #ff2100>640 nm</fc> | 100mW | |
| ==== Objectives ==== | ==== Objectives ==== | ||
| - | ^ Magnification ^ Immersion ^ NA ^ WD (mm) ^ Image Pixel Size ^ Reference ^ | + | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Image Pixel Size ^ |
| - | | 20x UPlan SAPO | - | 0.75 | 0.6 | 0.240 | | + | | <fc #0aa05c>20x</fc> | UPlan SAPO | - | 0.75 | 0.6 | 0.240 | |
| - | | 60x Plan APO N | Oil | 1.42 | 0.15 | 0.080* | | + | | <fc #2c80c0>60x</fc> | Plan APO N | Oil | 1.42 | 0.15 | 0.080* | |
| * after SIM reconstruction this system is capable of achieving resolutions of 100-150nm! | * after SIM reconstruction this system is capable of achieving resolutions of 100-150nm! | ||
| - | ==== Emission filters/cameras ==== | + | ==== Emission filters ==== |
| - | ^ Channel ^ Emission wavelength ^ Dyes ^ Camera ^ | + | |
| - | | DAPI | 435/31 | DAPI, CFP, Hoescht... | 1 | | + | ^ Channel ^ Wavelength ^ Camera ^ |
| - | | GFP | 528/48 | GFP, Alx488... | 1 | | + | | DAPI | <fc #2300ff>420-450 nm</fc> | 1 | |
| - | | RFP | 609/37 | RFP, Alx568... | 2 | | + | | GFP | <fc #56ff00>504-552 nm</fc> | 1 | |
| - | | Cy5 | 683/40 | GCy5, Alx633... | 2 | | + | | RFP | <fc #ff9f00>590-627 nm</fc> | 2 | |
| - | | DIC* | - | Brighfield | 1/2 | | + | | Cy5 | <fc #ff0000>663-703 nm</fc> | 2 | |
| + | | Brightfield / DIC* | - | 1/2 | | ||
| * though possible, DIC imaging is not recommended as it may introduce artifacts in the fluorescence reconstructions | * though possible, DIC imaging is not recommended as it may introduce artifacts in the fluorescence reconstructions | ||
| Line 60: | Line 61: | ||
| 2x PCO Edge 5.5 sCMOS 2560x2160 (note that each camera uses only 1024x1024 pixels to avoid field illumination problems and to allow simultaneous acquisition of 2 channels per camera) | 2x PCO Edge 5.5 sCMOS 2560x2160 (note that each camera uses only 1024x1024 pixels to avoid field illumination problems and to allow simultaneous acquisition of 2 channels per camera) | ||
| - | ===== Operating Procedures ===== | + | ===== Turning ON ===== |
| - | + | ||
| - | For more detailed information consult the full SOP/Guide here: | + | |
| - | (under construction) | + | |
| - | + | ||
| - | ==== Turning ON ==== | + | |
| * Turn on the main power switch at the top module | * Turn on the main power switch at the top module | ||
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| * Mount sample and start imaging. | * Mount sample and start imaging. | ||
| - | ==== Turning OFF ==== | + | ===== Turning OFF ===== |
| If you are NOT the last user for the day: | If you are NOT the last user for the day: | ||
deltavision.1736619215.txt.gz · Last modified: 2025/01/11 19:13 by bioimaging
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