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blaze [2026/02/17 17:03] bioimaging |
blaze [2026/05/26 11:47] (current) bioimaging [Microscope Turn On Procedure] |
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| ===== Laser Lines ===== | ===== Laser Lines ===== | ||
| - | ^ Wavelength (nm) ^ Fluorophores ^ Penetration capability ^ | + | ^ Wavelength ^ Fluorophores ^ Penetration capability ^ |
| - | | 488 | E.g: GFP, TRITC, AL488 | Less penetration | | + | | <fc #00f7ff>488 nm</fc> | E.g: GFP, TRITC, AL488 | Less penetration | |
| - | | 561 | E.g: mCherry, RFP, AL594 | Good penetration | | + | | <fc #c6ff00>561 nm</fc> | E.g: mCherry, RFP, AL594 | Good penetration | |
| - | | 640 | E.g: Cy5, TexasRed, AL647 | High penetration | | + | | <fc #ff2100>640 nm</fc> | E.g: Cy5, TexasRed, AL647 | High penetration | |
| - | | 785 | E.g: Atto740, Atto790 | Highest penetration | | + | | <fc #be0000>785 nm</fc> | E.g: Atto740, Atto790 | Highest penetration | |
| ===== Fluorescence filters ===== | ===== Fluorescence filters ===== | ||
| - | ^ Position ^ Filterset ^ Emission (nm) ^ | + | ^ Position ^ Filterset ^ Emission ^ |
| - | | 1 | 488 | 525/50 | | + | | 1 | 488 | <fc #4aff00>500-550 nm</fc> | |
| - | | 2 | 561 | 595/40 | | + | | 2 | 561 | <fc #ffcf00>575-615 nm</fc> | |
| - | | 3 | 640 | 680/30 | | + | | 3 | 640 | <fc #ff0000>665-695 nm</fc> | |
| - | | 4 | 785 | LP 805 | | + | | 4 | 785 | <fc #be0000> >805 nm</fc> | |
| | 5 | Empty | - | | | 5 | Empty | - | | ||
| | 6 | Empty | - | | | 6 | Empty | - | | ||
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| ===== Objectives ===== | ===== Objectives ===== | ||
| ^ Magnification ^ Dipping caps RI range ^ NA ^ WD per dipping cap (mm) ^ Maximum Theoretical Resolution (um) ^ | ^ Magnification ^ Dipping caps RI range ^ NA ^ WD per dipping cap (mm) ^ Maximum Theoretical Resolution (um) ^ | ||
| - | | 1.1x | 1.33-1.49 / 1.50-1.57 | 0.1 | 16 / 17.6 | 4.8 | | + | | <fc #88888a>1.1x</fc> | 1.33-1.49 / 1.50-1.57 | 0.1 | 16 / 17.6 | 4.8 | |
| - | | 4x | 1.33-1.41 / 1.42-1.48 / 1.49-1.57 | 0.35 | >=15 / >=15 / >=15 | 1.3 | | + | | <fc #df2b37>4x</fc> | 1.33-1.41 / 1.42-1.48 / 1.49-1.57 | 0.35 | >=15 / >=15 / >=15 | 1.3 | |
| - | | 12x | 1.33-1.41 / 1.42-1.48 / 1.49-1.57 | 0.55 | 8.5 / 10.9 / 10 | 0.5 | | + | | <fc #fef042>12x</fc> | 1.33-1.41 / 1.42-1.48 / 1.49-1.57 | 0.55 | 8.5 / 10.9 / 10 | 0.5 | |
| ===== Objectives + Zoom ===== | ===== Objectives + Zoom ===== | ||
| - | ^ Objective ^ Zoom ^ Final Magnification ^ Field of view (mm) ^ | + | ^ Objective ^ Zoom ^ Final Magnification ^ |
| - | | 1.1x | 0.6x | 0.66x | | | + | | <fc #88888a>1.1x</fc> | 0.6x | 0.66x | |
| - | | 1.1x | 1x | 1.1x | | | + | | <fc #88888a>1.1x</fc> | 1x | 1.1x | |
| - | | 1.1x | 1.66x | ~1.83x | | | + | | <fc #88888a>1.1x</fc> | 1.66x | ~1.83x | |
| - | | 1.1x | 2.5x | 2.75x | | | + | | <fc #88888a>1.1x</fc> | 2.5x | 2.75x | |
| - | | 4x | 0.6x | 2.4x | | | + | | <fc #df2b37>4x</fc> | 0.6x | 2.4x | |
| - | | 4x | 1x | 4x | | | + | | <fc #df2b37>4x</fc> | 1x | 4x | |
| - | | 4x | 1.66x | 6.64x | | | + | | <fc #df2b37>4x</fc> | 1.66x | 6.64x | |
| - | | 4x | 2.5x | 10x | | | + | | <fc #df2b37>4x</fc> | 2.5x | 10x | |
| - | | 12x | 0.6x | 7.2x | | | + | | <fc #fef042>12x</fc> | 0.6x | 7.2x | |
| - | | 12x | 1x | 12x | | | + | | <fc #fef042>12x</fc> | 1x | 12x | |
| - | | 12x | 1.66x | 19.92x | | | + | | <fc #fef042>12x</fc> | 1.66x | 19.92x | |
| - | | 12x | 2.5x | 30x | | | + | | <fc #fef042>12x</fc> | 2.5x | 30x | |
| ===== Camera ===== | ===== Camera ===== | ||
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| ===== Microscope Turn On Procedure ===== | ===== Microscope Turn On Procedure ===== | ||
| * Turn on the power bar on **top of the microscope's computer**, under the table. | * Turn on the power bar on **top of the microscope's computer**, under the table. | ||
| + | * | ||
| + | * Start the workstation. Booting will take a long time, and will only beep and turn the "ON" light after 2-3min - be patient! | ||
| ===== Microscope Turn Off Procedure ===== | ===== Microscope Turn Off Procedure ===== | ||
blaze.1771344192.txt.gz · Last modified: 2026/02/17 17:03 by bioimaging
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