blaze
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blaze [2025/01/13 15:53] bioimaging [Objectives] |
blaze [2025/02/03 12:51] (current) bioimaging |
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| Equipped with three objectives (1.1x, 4x and 12x) and internal magnifications of 0.6x, 1x, 1.66x and 2.5x, this system is capable of going from whole organs to single cell level. | Equipped with three objectives (1.1x, 4x and 12x) and internal magnifications of 0.6x, 1x, 1.66x and 2.5x, this system is capable of going from whole organs to single cell level. | ||
| There are 4 laser lines in the Blaze, 488, 561, 640 and 785 nm, and with the capability of combining these with any of the installed filters, the system is capable of mix-matching for markers such as Propidium Iodide. | There are 4 laser lines in the Blaze, 488, 561, 640 and 785 nm, and with the capability of combining these with any of the installed filters, the system is capable of mix-matching for markers such as Propidium Iodide. | ||
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| + | ===== Usage conditions ===== | ||
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| + | The **default configuration** for the UltraMicroscope Blaze is for the **Refractive Index of DBE** (iDisco protocol) at **RI = 1.56**. | ||
| + | Any use where another immersion media is necessary, the system will need realignment, and possibly undergo objective dipping-cap changes (below RI = 1.49). The **time taken to do these alignments** (DBE to immersion media, immersion media to DBE) can vary between 1-3 hours, and **will be charged to the user** in question. | ||
| ===== Laser Lines ===== | ===== Laser Lines ===== | ||
blaze.1736780011.txt.gz ยท Last modified: 2025/01/13 15:53 by bioimaging
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