blaze
Differences
This shows you the differences between two versions of the page.
| Both sides previous revision Previous revision Next revision | Previous revision | ||
|
blaze [2025/01/13 15:53] bioimaging [Camera] |
blaze [2025/02/03 12:51] (current) bioimaging |
||
|---|---|---|---|
| Line 28: | Line 28: | ||
| Equipped with three objectives (1.1x, 4x and 12x) and internal magnifications of 0.6x, 1x, 1.66x and 2.5x, this system is capable of going from whole organs to single cell level. | Equipped with three objectives (1.1x, 4x and 12x) and internal magnifications of 0.6x, 1x, 1.66x and 2.5x, this system is capable of going from whole organs to single cell level. | ||
| There are 4 laser lines in the Blaze, 488, 561, 640 and 785 nm, and with the capability of combining these with any of the installed filters, the system is capable of mix-matching for markers such as Propidium Iodide. | There are 4 laser lines in the Blaze, 488, 561, 640 and 785 nm, and with the capability of combining these with any of the installed filters, the system is capable of mix-matching for markers such as Propidium Iodide. | ||
| + | |||
| + | ===== Usage conditions ===== | ||
| + | |||
| + | The **default configuration** for the UltraMicroscope Blaze is for the **Refractive Index of DBE** (iDisco protocol) at **RI = 1.56**. | ||
| + | Any use where another immersion media is necessary, the system will need realignment, and possibly undergo objective dipping-cap changes (below RI = 1.49). The **time taken to do these alignments** (DBE to immersion media, immersion media to DBE) can vary between 1-3 hours, and **will be charged to the user** in question. | ||
| ===== Laser Lines ===== | ===== Laser Lines ===== | ||
| Line 49: | Line 54: | ||
| ===== Objectives ===== | ===== Objectives ===== | ||
| - | ^ Magnification ^ Dipping caps RI range ^ NA ^ WD per dipping cap (mm) ^ Maximum Theoretical Resolution (um) | + | ^ Magnification ^ Dipping caps RI range ^ NA ^ WD per dipping cap (mm) ^ Maximum Theoretical Resolution (um) ^ |
| | 1.1x | 1.33-1.49 / 1.50-1.57 | 0.1 | 16 / 17.6 | 4.8 | | | 1.1x | 1.33-1.49 / 1.50-1.57 | 0.1 | 16 / 17.6 | 4.8 | | ||
| | 4x | 1.33-1.41 / 1.42-1.48 / 1.49-1.57 | 0.35 | >=15 / >=15 / >=15 | 1.3 | | | 4x | 1.33-1.41 / 1.42-1.48 / 1.49-1.57 | 0.35 | >=15 / >=15 / >=15 | 1.3 | | ||
blaze.1736779982.txt.gz ยท Last modified: 2025/01/13 15:53 by bioimaging
Page Tools
