blaze
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blaze [2025/01/13 13:41] bioimaging [Fluorescence filters] |
blaze [2025/02/03 12:51] (current) bioimaging |
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| Equipped with three objectives (1.1x, 4x and 12x) and internal magnifications of 0.6x, 1x, 1.66x and 2.5x, this system is capable of going from whole organs to single cell level. | Equipped with three objectives (1.1x, 4x and 12x) and internal magnifications of 0.6x, 1x, 1.66x and 2.5x, this system is capable of going from whole organs to single cell level. | ||
| There are 4 laser lines in the Blaze, 488, 561, 640 and 785 nm, and with the capability of combining these with any of the installed filters, the system is capable of mix-matching for markers such as Propidium Iodide. | There are 4 laser lines in the Blaze, 488, 561, 640 and 785 nm, and with the capability of combining these with any of the installed filters, the system is capable of mix-matching for markers such as Propidium Iodide. | ||
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| + | ===== Usage conditions ===== | ||
| + | |||
| + | The **default configuration** for the UltraMicroscope Blaze is for the **Refractive Index of DBE** (iDisco protocol) at **RI = 1.56**. | ||
| + | Any use where another immersion media is necessary, the system will need realignment, and possibly undergo objective dipping-cap changes (below RI = 1.49). The **time taken to do these alignments** (DBE to immersion media, immersion media to DBE) can vary between 1-3 hours, and **will be charged to the user** in question. | ||
| ===== Laser Lines ===== | ===== Laser Lines ===== | ||
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| | 3 | 640 | 680/30 | | | 3 | 640 | 680/30 | | ||
| | 4 | 785 | LP 805 | | | 4 | 785 | LP 805 | | ||
| - | | 5 | - | Empty | | + | | 5 | Empty | - | |
| - | | 6 | - | Empty | | + | | 6 | Empty | - | |
| - | | 7 | - | Empty | | + | | 7 | Empty | - | |
| ===== Objectives ===== | ===== Objectives ===== | ||
| - | ^ Magnification ^ Dipping caps RI range ^ NA ^ WD per dipping cap (mm) ^ Maximum Theoretical Resolution (um) | + | ^ Magnification ^ Dipping caps RI range ^ NA ^ WD per dipping cap (mm) ^ Maximum Theoretical Resolution (um) ^ |
| | 1.1x | 1.33-1.49 / 1.50-1.57 | 0.1 | 16 / 17.6 | 4.8 | | | 1.1x | 1.33-1.49 / 1.50-1.57 | 0.1 | 16 / 17.6 | 4.8 | | ||
| | 4x | 1.33-1.41 / 1.42-1.48 / 1.49-1.57 | 0.35 | >=15 / >=15 / >=15 | 1.3 | | | 4x | 1.33-1.41 / 1.42-1.48 / 1.49-1.57 | 0.35 | >=15 / >=15 / >=15 | 1.3 | | ||
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| ^ Model ^ Frame Size ^ Pixel Size (µm) ^ Quantum Efficiency ^ | ^ Model ^ Frame Size ^ Pixel Size (µm) ^ Quantum Efficiency ^ | ||
| | [[https://www.excelitas.com/product/pcoedge-55-usb-scmos-camera|PCO Edge 5.5 sCMOS]] | 2048 × 2048 | 6.5 x 6.5 | 60% | | | [[https://www.excelitas.com/product/pcoedge-55-usb-scmos-camera|PCO Edge 5.5 sCMOS]] | 2048 × 2048 | 6.5 x 6.5 | 60% | | ||
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| + | ===== Microscope Turn On Procedure ===== | ||
| + | * Turn on the power bar on **top of the microscope's computer**, under the table. | ||
| + | |||
| + | ===== Microscope Turn Off Procedure ===== | ||
| + | * With the software still open, **pull out the cuvette drawer**. | ||
| + | * Wait for the **drawer** to move **all the way down**. | ||
| + | * **Remove any used cuvette**. | ||
| + | * **Press the drawer button**, but **do not push the drawer** in. | ||
| + | * In software, **change to 12x objective** and **lower the objectives all the way**. | ||
| + | * Turn off the power bar on **top of the microscope's computer**, under the table. | ||
| + | * **Close** the cuvette **drawer and software**. | ||
| ---- | ---- | ||
| [[resources|Back to the Equipment page]] | [[resources|Back to the Equipment page]] | ||
blaze.1736772095.txt.gz · Last modified: 2025/01/13 13:41 by bioimaging
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