blaze
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blaze [2025/01/13 12:53] bioimaging |
blaze [2025/02/03 12:51] (current) bioimaging |
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| <b>Year</b>: 2024 <br> | <b>Year</b>: 2024 <br> | ||
| <b>SN</b>: <br> | <b>SN</b>: <br> | ||
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| - | Data will be deleted after: <b>1 month</b> | ||
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| → <a href="/facility/bioimaging/doku.php?id=blaze_quality"><img src="/facility/bioimaging/lib/exe/fetch.php?media=list-icon.png" width=18> Miltenyi UltraMicroscope Blaze Quality Control</a> | → <a href="/facility/bioimaging/doku.php?id=blaze_quality"><img src="/facility/bioimaging/lib/exe/fetch.php?media=list-icon.png" width=18> Miltenyi UltraMicroscope Blaze Quality Control</a> | ||
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| The UltraMicroscope Blaze is a lightsheet for large cleared samples system with dual sided illumination, capable of using three beams per side. This allows for uniform imaging with minimal shadows (in good sample clearing conditions). | The UltraMicroscope Blaze is a lightsheet for large cleared samples system with dual sided illumination, capable of using three beams per side. This allows for uniform imaging with minimal shadows (in good sample clearing conditions). | ||
| - | Equipped with three objectives (1x, 4x and 12x) and internal magnifications of 0.6x, 1.2x, 1.66x and 2.5x, this system is capable of going from whole organs to single cell level. | + | Equipped with three objectives (1.1x, 4x and 12x) and internal magnifications of 0.6x, 1x, 1.66x and 2.5x, this system is capable of going from whole organs to single cell level. |
| There are 4 laser lines in the Blaze, 488, 561, 640 and 785 nm, and with the capability of combining these with any of the installed filters, the system is capable of mix-matching for markers such as Propidium Iodide. | There are 4 laser lines in the Blaze, 488, 561, 640 and 785 nm, and with the capability of combining these with any of the installed filters, the system is capable of mix-matching for markers such as Propidium Iodide. | ||
| - | ===== System components ===== | + | ===== Usage conditions ===== |
| - | ^ Position ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ | + | The **default configuration** for the UltraMicroscope Blaze is for the **Refractive Index of DBE** (iDisco protocol) at **RI = 1.56**. |
| - | | | | | | | | | + | Any use where another immersion media is necessary, the system will need realignment, and possibly undergo objective dipping-cap changes (below RI = 1.49). The **time taken to do these alignments** (DBE to immersion media, immersion media to DBE) can vary between 1-3 hours, and **will be charged to the user** in question. |
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| + | ===== Laser Lines ===== | ||
| - | ==== Objectives ==== | + | ^ Wavelength (nm) ^ Fluorophores ^ Penetration capability ^ |
| - | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | + | | 488 | E.g: GFP, TRITC, AL488 | Less penetration | |
| - | | | | | | | | + | | 561 | E.g: mCherry, RFP, AL594 | Good penetration | |
| + | | 640 | E.g: Cy5, TexasRed, AL647 | High penetration | | ||
| + | | 785 | E.g: Atto740, Atto790 | Highest penetration | | ||
| - | ==== Camera ==== | + | ===== Fluorescence filters ===== |
| + | ^ Position ^ Filterset ^ Emission (nm) ^ | ||
| + | | 1 | 488 | 525/50 | | ||
| + | | 2 | 561 | 595/40 | | ||
| + | | 3 | 640 | 680/30 | | ||
| + | | 4 | 785 | LP 805 | | ||
| + | | 5 | Empty | - | | ||
| + | | 6 | Empty | - | | ||
| + | | 7 | Empty | - | | ||
| + | |||
| + | ===== Objectives ===== | ||
| + | ^ Magnification ^ Dipping caps RI range ^ NA ^ WD per dipping cap (mm) ^ Maximum Theoretical Resolution (um) ^ | ||
| + | | 1.1x | 1.33-1.49 / 1.50-1.57 | 0.1 | 16 / 17.6 | 4.8 | | ||
| + | | 4x | 1.33-1.41 / 1.42-1.48 / 1.49-1.57 | 0.35 | >=15 / >=15 / >=15 | 1.3 | | ||
| + | | 12x | 1.33-1.41 / 1.42-1.48 / 1.49-1.57 | 0.55 | 8.5 / 10.9 / 10 | 0.5 | | ||
| + | |||
| + | ===== Objectives + Zoom ===== | ||
| + | ^ Objective ^ Zoom ^ Final Magnification ^ Field of view (mm) ^ | ||
| + | | 1.1x | 0.6x | 0.66x | | | ||
| + | | 1.1x | 1x | 1.1x | | | ||
| + | | 1.1x | 1.66x | ~1.83x | | | ||
| + | | 1.1x | 2.5x | 2.75x | | | ||
| + | | 4x | 0.6x | 2.4x | | | ||
| + | | 4x | 1x | 4x | | | ||
| + | | 4x | 1.66x | 6.64x | | | ||
| + | | 4x | 2.5x | 10x | | | ||
| + | | 12x | 0.6x | 7.2x | | | ||
| + | | 12x | 1x | 12x | | | ||
| + | | 12x | 1.66x | 19.92x | | | ||
| + | | 12x | 2.5x | 30x | | | ||
| + | |||
| + | ===== Camera ===== | ||
| + | |||
| + | ^ Model ^ Frame Size ^ Pixel Size (µm) ^ Quantum Efficiency ^ | ||
| + | | [[https://www.excelitas.com/product/pcoedge-55-usb-scmos-camera|PCO Edge 5.5 sCMOS]] | 2048 × 2048 | 6.5 x 6.5 | 60% | | ||
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| + | ===== Microscope Turn On Procedure ===== | ||
| + | * Turn on the power bar on **top of the microscope's computer**, under the table. | ||
| + | |||
| + | ===== Microscope Turn Off Procedure ===== | ||
| + | * With the software still open, **pull out the cuvette drawer**. | ||
| + | * Wait for the **drawer** to move **all the way down**. | ||
| + | * **Remove any used cuvette**. | ||
| + | * **Press the drawer button**, but **do not push the drawer** in. | ||
| + | * In software, **change to 12x objective** and **lower the objectives all the way**. | ||
| + | * Turn off the power bar on **top of the microscope's computer**, under the table. | ||
| + | * **Close** the cuvette **drawer and software**. | ||
| ---- | ---- | ||
| [[resources|Back to the Equipment page]] | [[resources|Back to the Equipment page]] | ||
blaze.1736769218.txt.gz · Last modified: 2025/01/13 12:53 by bioimaging
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