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agilent_cytation_5 [2025/12/12 01:13] bioimaging |
agilent_cytation_5 [2026/08/05 15:41] (current) bioimaging [Filtersets available] |
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| <a href="/facility/bioimaging/lib/exe/fetch.php?media=cytation.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=cytation.jpg" width=300></a> | <a href="/facility/bioimaging/lib/exe/fetch.php?media=cytation.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=cytation.jpg" width=300></a> | ||
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| - | <b>Location</b>: [Oeiras] Room 0B16<br> | + | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=bartolomeu_dias_wing_2025_-_cytation_5.png">[Oeiras] Room 0B16</a><br> |
| <b>Manufacturer</b>: <a href="https://www.agilent.com/" target="_blank">Agilent</a> <br> | <b>Manufacturer</b>: <a href="https://www.agilent.com/" target="_blank">Agilent</a> <br> | ||
| <b>Model</b>: <a href="https://www.agilent.com/en/product/cell-analysis/cell-imaging-microscopy/cell-imaging-multimode-readers/biotek-cytation-5-cell-imaging-multimode-reader-1623202" target="_blank">BioTek Cytation 5 Cell Imaging Multimode Reader</a> <br> | <b>Model</b>: <a href="https://www.agilent.com/en/product/cell-analysis/cell-imaging-microscopy/cell-imaging-multimode-readers/biotek-cytation-5-cell-imaging-multimode-reader-1623202" target="_blank">BioTek Cytation 5 Cell Imaging Multimode Reader</a> <br> | ||
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| | - | GFP | 453-488 nm | 497 nm | <fc #4aff00>505-545 nm</fc> |[[https://www.agilent.com/store/en_US/Prod-1225101/1225101|1225101]] | | | - | GFP | 453-488 nm | 497 nm | <fc #4aff00>505-545 nm</fc> |[[https://www.agilent.com/store/en_US/Prod-1225101/1225101|1225101]] | | ||
| | 3 | Propidium Iodide | 511-551 nm | 605 nm | <fc #ff0000>619-676 nm</fc> |[[https://www.agilent.com/store/en_US/Prod-1225111/1225111|1225111]] | | | 3 | Propidium Iodide | 511-551 nm | 605 nm | <fc #ff0000>619-676 nm</fc> |[[https://www.agilent.com/store/en_US/Prod-1225111/1225111|1225111]] | | ||
| - | | - | Texas Red | 579-594 nm | 605 nm | <fc #ff0000>619-676 nm</fc> | [[https://www.agilent.com/store/en_US/Prod-1225102/1225102|1225102]] | | + | | 4 | Texas Red | 579-594 nm | 605 nm | <fc #ff0000>619-676 nm</fc> | [[https://www.agilent.com/store/en_US/Prod-1225102/1225102|1225102]] | |
| - | | 4 | Cy5 | 608-648 nm | 660 nm | <fc #ff0000>665-705 nm</fc> | [[https://www.agilent.com/store/en_US/Prod-1225105/|1225105]] | | + | | - | Cy5 | 608-648 nm | 660 nm | <fc #ff0000>665-705 nm</fc> | [[https://www.agilent.com/store/en_US/Prod-1225105/|1225105]] | |
| ==== Objectives ==== | ==== Objectives ==== | ||
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| | [[https://www.teledynevisionsolutions.com/products/chameleon3-usb3/?model=CM3-U3-50S5M-CS&vertical=machine%20vision&segment=iis|FLIR Chameleon3 CM3-U3-50S5M (mono)]] | 2448 × 2048 | 3.45 x 3.45 | 69% (at 525 nm) | | | [[https://www.teledynevisionsolutions.com/products/chameleon3-usb3/?model=CM3-U3-50S5M-CS&vertical=machine%20vision&segment=iis|FLIR Chameleon3 CM3-U3-50S5M (mono)]] | 2448 × 2048 | 3.45 x 3.45 | 69% (at 525 nm) | | ||
| - | ===== Pixel Size ===== | + | ==== Pixel Size ==== |
| Images coming from the Cytation 5 are not calibrated, even though the system has that information available. In the images' metadata you can find the PixelWidth and the ImageWidthMicrons, and by dividing the latter for the first, you get the Pixel Size to calibrate your images. | Images coming from the Cytation 5 are not calibrated, even though the system has that information available. In the images' metadata you can find the PixelWidth and the ImageWidthMicrons, and by dividing the latter for the first, you get the Pixel Size to calibrate your images. | ||
agilent_cytation_5.1765498388.txt.gz · Last modified: 2025/12/12 01:13 by bioimaging
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