agilent_cytation_5
Differences
This shows you the differences between two versions of the page.
| Both sides previous revision Previous revision Next revision | Previous revision | ||
|
agilent_cytation_5 [2025/01/08 16:33] bioimaging [CO2/O2 Control Procedure] |
agilent_cytation_5 [2025/03/10 16:45] (current) bioimaging |
||
|---|---|---|---|
| Line 12: | Line 12: | ||
| <b>Software</b>: Gen 5 Image Prime <br> | <b>Software</b>: Gen 5 Image Prime <br> | ||
| <b>Year</b>: 2024 <br> | <b>Year</b>: 2024 <br> | ||
| - | <b>SN</b>: <br> | + | <b>SN</b>: JDGD GHWD HGWM KH<br> |
|   <br> |   <br> | ||
| Data will be deleted after: <b>1 month</b> | Data will be deleted after: <b>1 month</b> | ||
| Line 37: | Line 37: | ||
| ===== Filtersets available ===== | ===== Filtersets available ===== | ||
| - | ^ Default Config ^ Filterset ^ Filterset Reference ^ LED wavelength (nm) ^ LED reference ^ Excitation (nm) ^ Dichroic (nm) ^ Emission (nm) ^ Default Config ^ | + | ^ Default Config ^ Filterset ^ Filterset Reference ^ LED wavelength (nm) ^ LED reference ^ Excitation (nm) ^ Dichroic (nm) ^ Emission (nm) ^ |
| | Pos 1 | Laser Autofocus | [[https://www.agilent.com/store/productDetail.jsp?catalogId=1225010&catId=SubCat2ECS_2041516|1225010]] | - | - | - | - | - | | | Pos 1 | Laser Autofocus | [[https://www.agilent.com/store/productDetail.jsp?catalogId=1225010&catId=SubCat2ECS_2041516|1225010]] | - | - | - | - | - | | ||
| | Pos 2 | DAPI | [[https://www.agilent.com/store/en_US/Prod-1225100/1225100|1225100]] | 365 | 1225007 | 377/50 | 409 | 447/60 | | | Pos 2 | DAPI | [[https://www.agilent.com/store/en_US/Prod-1225100/1225100|1225100]] | 365 | 1225007 | 377/50 | 409 | 447/60 | | ||
| Line 57: | Line 57: | ||
| ^ Model ^ Frame Size ^ Pixel Size (µm) ^ Quantum Efficiency ^ | ^ Model ^ Frame Size ^ Pixel Size (µm) ^ Quantum Efficiency ^ | ||
| | [[https://www.teledynevisionsolutions.com/products/chameleon3-usb3/?model=CM3-U3-50S5M-CS&vertical=machine%20vision&segment=iis|FLIR Chameleon3 CM3-U3-50S5M (mono)]] | 2448 × 2048 | 3.45 x 3.45 | 69% (at 525 nm) | | | [[https://www.teledynevisionsolutions.com/products/chameleon3-usb3/?model=CM3-U3-50S5M-CS&vertical=machine%20vision&segment=iis|FLIR Chameleon3 CM3-U3-50S5M (mono)]] | 2448 × 2048 | 3.45 x 3.45 | 69% (at 525 nm) | | ||
| + | |||
| + | |||
| + | ===== Pixel Size ===== | ||
| + | |||
| + | Images coming from the Cytation 5 are not calibrated, even though the system has that information available. In the images' metadata you can find the PixelWidth and the ImageWidthMicrons, and by dividing the latter for the first, you get the Pixel Size to calibrate your images. | ||
| + | You can find this information below, per objective and Field Of View (FOV): | ||
| + | |||
| + | ^ Objective ^ FOV ^ Image Size (pixels) ^ Image Size (microns) ^ Pixel Size (microns) ^ | ||
| + | | 4x | Full | 1992 x 1992 | 3474 x 3474 | ~1.7440 x ~1.7440 | | ||
| + | | 4x | 75% | 1496 x 1496 | 2609 x 2609 | ~1.7440 x ~1.7440 | | ||
| + | | 4x | Standard | 1224 x 904 | 2135 x 1576 | ~1.7443 x ~1.7434 | | ||
| + | | 20x | Full | 1992 x 1992 | 694 x 694 | ~0.3484 x ~0.3484 | | ||
| + | | 20x | 75% | 1496 x 1496 | 521 x 521 | ~0.3483 x ~0.3483 | | ||
| + | | 20x | Standard | 1224 x 904 | 427 x 315 | ~0.3489 x ~0.3485 | | ||
| + | | 40x | Full | 1992 x 1992 | 347 x 347 | ~0.1742 x ~0.1742 | | ||
| + | | 40x | 75% | 1496 x 1496 | 260 x 260 | ~0.1738 x ~0.1738 | | ||
| + | | 40x | Standard | 1224 x 904 | 213 x 157 | ~0.1740 x ~0.1737 | | ||
agilent_cytation_5.1736350415.txt.gz · Last modified: 2025/01/08 16:33 by bioimaging
Page Tools
