agilent_cytation_5
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agilent_cytation_5 [2025/01/08 16:26] bioimaging [CO2/O2 Control Procedure] |
agilent_cytation_5 [2025/03/10 16:45] (current) bioimaging |
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| <b>Software</b>: Gen 5 Image Prime <br> | <b>Software</b>: Gen 5 Image Prime <br> | ||
| <b>Year</b>: 2024 <br> | <b>Year</b>: 2024 <br> | ||
| - | <b>SN</b>: <br> | + | <b>SN</b>: JDGD GHWD HGWM KH<br> |
|   <br> |   <br> | ||
| Data will be deleted after: <b>1 month</b> | Data will be deleted after: <b>1 month</b> | ||
| Line 37: | Line 37: | ||
| ===== Filtersets available ===== | ===== Filtersets available ===== | ||
| - | ^ Default Config ^ Filterset ^ Filterset Reference ^ LED wavelength (nm) ^ LED reference ^ Excitation (nm) ^ Dichroic (nm) ^ Emission (nm) ^ Default Config ^ | + | ^ Default Config ^ Filterset ^ Filterset Reference ^ LED wavelength (nm) ^ LED reference ^ Excitation (nm) ^ Dichroic (nm) ^ Emission (nm) ^ |
| | Pos 1 | Laser Autofocus | [[https://www.agilent.com/store/productDetail.jsp?catalogId=1225010&catId=SubCat2ECS_2041516|1225010]] | - | - | - | - | - | | | Pos 1 | Laser Autofocus | [[https://www.agilent.com/store/productDetail.jsp?catalogId=1225010&catId=SubCat2ECS_2041516|1225010]] | - | - | - | - | - | | ||
| | Pos 2 | DAPI | [[https://www.agilent.com/store/en_US/Prod-1225100/1225100|1225100]] | 365 | 1225007 | 377/50 | 409 | 447/60 | | | Pos 2 | DAPI | [[https://www.agilent.com/store/en_US/Prod-1225100/1225100|1225100]] | 365 | 1225007 | 377/50 | 409 | 447/60 | | ||
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| - | ===== CO2/O2 Control Procedure ===== | + | ===== Pixel Size ===== |
| - | * **If you require CO2/O2 control**, turn the **atmosphere controller ON**. | + | Images coming from the Cytation 5 are not calibrated, even though the system has that information available. In the images' metadata you can find the PixelWidth and the ImageWidthMicrons, and by dividing the latter for the first, you get the Pixel Size to calibrate your images. |
| + | You can find this information below, per objective and Field Of View (FOV): | ||
| + | |||
| + | ^ Objective ^ FOV ^ Image Size (pixels) ^ Image Size (microns) ^ Pixel Size (microns) ^ | ||
| + | | 4x | Full | 1992 x 1992 | 3474 x 3474 | ~1.7440 x ~1.7440 | | ||
| + | | 4x | 75% | 1496 x 1496 | 2609 x 2609 | ~1.7440 x ~1.7440 | | ||
| + | | 4x | Standard | 1224 x 904 | 2135 x 1576 | ~1.7443 x ~1.7434 | | ||
| + | | 20x | Full | 1992 x 1992 | 694 x 694 | ~0.3484 x ~0.3484 | | ||
| + | | 20x | 75% | 1496 x 1496 | 521 x 521 | ~0.3483 x ~0.3483 | | ||
| + | | 20x | Standard | 1224 x 904 | 427 x 315 | ~0.3489 x ~0.3485 | | ||
| + | | 40x | Full | 1992 x 1992 | 347 x 347 | ~0.1742 x ~0.1742 | | ||
| + | | 40x | 75% | 1496 x 1496 | 260 x 260 | ~0.1738 x ~0.1738 | | ||
| + | | 40x | Standard | 1224 x 904 | 213 x 157 | ~0.1740 x ~0.1737 | | ||
| + | |||
| + | |||
| + | ===== CO2 and/or O2 Control Procedure ===== | ||
| + | |||
| + | * **If you require CO2 and/or O2 control**, turn the **atmosphere controller ON**. | ||
| {{cytation_gas_1.png?200|}} | {{cytation_gas_1.png?200|}} | ||
| - | * Confirm the **CO2/O2 switch is ON**, and set to the desired percentage. | + | * Confirm the **CO2 and/or O2 switch is ON**, and set to the desired percentage. |
| - | {{cytation_gas_2.png?200|}} | + | {{cytation_gas_2.png?600|}} |
| - | * **Open the CO2/N2 tap** on the wall. | + | * **Open the CO2 and/or N2 tap** on the wall. |
| {{cytation_gas_3.png?200|}} | {{cytation_gas_3.png?200|}} | ||
agilent_cytation_5.1736350008.txt.gz · Last modified: 2025/01/08 16:26 by bioimaging
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