agilent_cytation_5
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agilent_cytation_5 [2025/01/08 16:14] bioimaging [Camera] |
agilent_cytation_5 [2025/03/10 16:45] (current) bioimaging |
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| <b>Software</b>: Gen 5 Image Prime <br> | <b>Software</b>: Gen 5 Image Prime <br> | ||
| <b>Year</b>: 2024 <br> | <b>Year</b>: 2024 <br> | ||
| - | <b>SN</b>: <br> | + | <b>SN</b>: JDGD GHWD HGWM KH<br> |
|   <br> |   <br> | ||
| Data will be deleted after: <b>1 month</b> | Data will be deleted after: <b>1 month</b> | ||
| Line 37: | Line 37: | ||
| ===== Filtersets available ===== | ===== Filtersets available ===== | ||
| - | ^ Default Config ^ Filterset ^ Filterset Reference ^ LED wavelength (nm) ^ LED reference ^ Excitation (nm) ^ Dichroic (nm) ^ Emission (nm) ^ Default Config ^ | + | ^ Default Config ^ Filterset ^ Filterset Reference ^ LED wavelength (nm) ^ LED reference ^ Excitation (nm) ^ Dichroic (nm) ^ Emission (nm) ^ |
| | Pos 1 | Laser Autofocus | [[https://www.agilent.com/store/productDetail.jsp?catalogId=1225010&catId=SubCat2ECS_2041516|1225010]] | - | - | - | - | - | | | Pos 1 | Laser Autofocus | [[https://www.agilent.com/store/productDetail.jsp?catalogId=1225010&catId=SubCat2ECS_2041516|1225010]] | - | - | - | - | - | | ||
| | Pos 2 | DAPI | [[https://www.agilent.com/store/en_US/Prod-1225100/1225100|1225100]] | 365 | 1225007 | 377/50 | 409 | 447/60 | | | Pos 2 | DAPI | [[https://www.agilent.com/store/en_US/Prod-1225100/1225100|1225100]] | 365 | 1225007 | 377/50 | 409 | 447/60 | | ||
| Line 59: | Line 59: | ||
| + | ===== Pixel Size ===== | ||
| + | Images coming from the Cytation 5 are not calibrated, even though the system has that information available. In the images' metadata you can find the PixelWidth and the ImageWidthMicrons, and by dividing the latter for the first, you get the Pixel Size to calibrate your images. | ||
| + | You can find this information below, per objective and Field Of View (FOV): | ||
| + | ^ Objective ^ FOV ^ Image Size (pixels) ^ Image Size (microns) ^ Pixel Size (microns) ^ | ||
| + | | 4x | Full | 1992 x 1992 | 3474 x 3474 | ~1.7440 x ~1.7440 | | ||
| + | | 4x | 75% | 1496 x 1496 | 2609 x 2609 | ~1.7440 x ~1.7440 | | ||
| + | | 4x | Standard | 1224 x 904 | 2135 x 1576 | ~1.7443 x ~1.7434 | | ||
| + | | 20x | Full | 1992 x 1992 | 694 x 694 | ~0.3484 x ~0.3484 | | ||
| + | | 20x | 75% | 1496 x 1496 | 521 x 521 | ~0.3483 x ~0.3483 | | ||
| + | | 20x | Standard | 1224 x 904 | 427 x 315 | ~0.3489 x ~0.3485 | | ||
| + | | 40x | Full | 1992 x 1992 | 347 x 347 | ~0.1742 x ~0.1742 | | ||
| + | | 40x | 75% | 1496 x 1496 | 260 x 260 | ~0.1738 x ~0.1738 | | ||
| + | | 40x | Standard | 1224 x 904 | 213 x 157 | ~0.1740 x ~0.1737 | | ||
| - | ===== CO2/O2 Control Procedure ===== | + | ===== CO2 and/or O2 Control Procedure ===== |
| - | * **If you require CO2/O2 control**, turn the **atmosphere controller ON**. | + | * **If you require CO2 and/or O2 control**, turn the **atmosphere controller ON**. |
| - | {{cytation_gas_1.png?|}} | + | {{cytation_gas_1.png?200|}} |
| - | * Confirm the **CO2/O2 switch is ON**, and set to the desired percentage. | + | * Confirm the **CO2 and/or O2 switch is ON**, and set to the desired percentage. |
| - | {{cytation_gas_2.png?|}} | + | {{cytation_gas_2.png?600|}} |
| - | * **Open the CO2/N2 tap** on the wall. | + | * **Open the CO2 and/or N2 tap** on the wall. |
| - | {{cytation_gas_3.png?|}} | + | {{cytation_gas_3.png?200|}} |
| Line 76: | Line 89: | ||
| * Turn on the microscope using the **switch** at the **front of the system**. | * Turn on the microscope using the **switch** at the **front of the system**. | ||
| - | {{cytation_turnon_1.png?|}} | + | {{cytation_turnon_1.png?200|}} |
| * Turn the **computer** on. | * Turn the **computer** on. | ||
| - | {{cytation_turnon_2.png?|}} | + | {{cytation_turnon_2.png?200|}} |
| * **Login** to Windows with your **GIMM Agendo credentials**. | * **Login** to Windows with your **GIMM Agendo credentials**. | ||
| * Wait for the **button light** in the front to **turn green** (the system will pop out the plate drawer as well). | * Wait for the **button light** in the front to **turn green** (the system will pop out the plate drawer as well). | ||
| - | {{cytation_turnon_3.png?|}} | + | {{cytation_turnon_3.png?200|}} |
| * Open the **Gen 5** software. | * Open the **Gen 5** software. | ||
| Line 89: | Line 102: | ||
| * Turn off the microscope using the **switch** at the **front of the system**. | * Turn off the microscope using the **switch** at the **front of the system**. | ||
| - | {{cytation_turnon_1.png?|}} | + | {{cytation_turnon_1.png?200|}} |
| * **Sign out** of the computer. | * **Sign out** of the computer. | ||
| * **If you used the gas controller** | * **If you used the gas controller** | ||
| * Turn gas controller OFF | * Turn gas controller OFF | ||
| - | {{cytation_gas_1.png?|}} | + | {{cytation_gas_1.png?200|}} |
| * Close all wall taps. | * Close all wall taps. | ||
| - | {{cytation_gas_3.png?|}} | + | {{cytation_gas_3.png?200|}} |
| ---- | ---- | ||
| [[resources|Back to the Equipment page]] | [[resources|Back to the Equipment page]] | ||
agilent_cytation_5.1736349293.txt.gz · Last modified: 2025/01/08 16:14 by bioimaging
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