agilent_cytation_5
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agilent_cytation_5 [2025/01/08 16:07] bioimaging [Microscope Turn On Procedure] |
agilent_cytation_5 [2025/03/10 16:45] (current) bioimaging |
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| <b>Software</b>: Gen 5 Image Prime <br> | <b>Software</b>: Gen 5 Image Prime <br> | ||
| <b>Year</b>: 2024 <br> | <b>Year</b>: 2024 <br> | ||
| - | <b>SN</b>: <br> | + | <b>SN</b>: JDGD GHWD HGWM KH<br> |
|   <br> |   <br> | ||
| Data will be deleted after: <b>1 month</b> | Data will be deleted after: <b>1 month</b> | ||
| Line 37: | Line 37: | ||
| ===== Filtersets available ===== | ===== Filtersets available ===== | ||
| - | ^ Default Config ^ Filterset ^ Filterset Reference ^ LED wavelength (nm) ^ LED reference ^ Excitation (nm) ^ Dichroic (nm) ^ Emission (nm) ^ Default Config ^ | + | ^ Default Config ^ Filterset ^ Filterset Reference ^ LED wavelength (nm) ^ LED reference ^ Excitation (nm) ^ Dichroic (nm) ^ Emission (nm) ^ |
| | Pos 1 | Laser Autofocus | [[https://www.agilent.com/store/productDetail.jsp?catalogId=1225010&catId=SubCat2ECS_2041516|1225010]] | - | - | - | - | - | | | Pos 1 | Laser Autofocus | [[https://www.agilent.com/store/productDetail.jsp?catalogId=1225010&catId=SubCat2ECS_2041516|1225010]] | - | - | - | - | - | | ||
| | Pos 2 | DAPI | [[https://www.agilent.com/store/en_US/Prod-1225100/1225100|1225100]] | 365 | 1225007 | 377/50 | 409 | 447/60 | | | Pos 2 | DAPI | [[https://www.agilent.com/store/en_US/Prod-1225100/1225100|1225100]] | 365 | 1225007 | 377/50 | 409 | 447/60 | | ||
| Line 59: | Line 59: | ||
| - | ===== CO2/O2 Control Procedure ===== | + | ===== Pixel Size ===== |
| - | * **If you require CO2/O2 control**, turn the **atmosphere controller ON**. | + | Images coming from the Cytation 5 are not calibrated, even though the system has that information available. In the images' metadata you can find the PixelWidth and the ImageWidthMicrons, and by dividing the latter for the first, you get the Pixel Size to calibrate your images. |
| - | {{cytation_gas_1.png?|}} | + | You can find this information below, per objective and Field Of View (FOV): |
| - | * Confirm the **CO2/O2 switch is ON**, and set to the desired percentage. | + | |
| - | {{cytation_gas_2.png?|}} | + | ^ Objective ^ FOV ^ Image Size (pixels) ^ Image Size (microns) ^ Pixel Size (microns) ^ |
| - | * **Open the CO2/N2 tap** on the wall. | + | | 4x | Full | 1992 x 1992 | 3474 x 3474 | ~1.7440 x ~1.7440 | |
| - | {{cytation_gas_3.png?|}} | + | | 4x | 75% | 1496 x 1496 | 2609 x 2609 | ~1.7440 x ~1.7440 | |
| + | | 4x | Standard | 1224 x 904 | 2135 x 1576 | ~1.7443 x ~1.7434 | | ||
| + | | 20x | Full | 1992 x 1992 | 694 x 694 | ~0.3484 x ~0.3484 | | ||
| + | | 20x | 75% | 1496 x 1496 | 521 x 521 | ~0.3483 x ~0.3483 | | ||
| + | | 20x | Standard | 1224 x 904 | 427 x 315 | ~0.3489 x ~0.3485 | | ||
| + | | 40x | Full | 1992 x 1992 | 347 x 347 | ~0.1742 x ~0.1742 | | ||
| + | | 40x | 75% | 1496 x 1496 | 260 x 260 | ~0.1738 x ~0.1738 | | ||
| + | | 40x | Standard | 1224 x 904 | 213 x 157 | ~0.1740 x ~0.1737 | | ||
| + | |||
| + | |||
| + | ===== CO2 and/or O2 Control Procedure ===== | ||
| + | |||
| + | * **If you require CO2 and/or O2 control**, turn the **atmosphere controller ON**. | ||
| + | {{cytation_gas_1.png?200|}} | ||
| + | * Confirm the **CO2 and/or O2 switch is ON**, and set to the desired percentage. | ||
| + | {{cytation_gas_2.png?600|}} | ||
| + | * **Open the CO2 and/or N2 tap** on the wall. | ||
| + | {{cytation_gas_3.png?200|}} | ||
| Line 72: | Line 89: | ||
| * Turn on the microscope using the **switch** at the **front of the system**. | * Turn on the microscope using the **switch** at the **front of the system**. | ||
| - | {{cytation_turnon_1.png?|}} | + | {{cytation_turnon_1.png?200|}} |
| * Turn the **computer** on. | * Turn the **computer** on. | ||
| - | {{cytation_turnon_2.png?|}} | + | {{cytation_turnon_2.png?200|}} |
| * **Login** to Windows with your **GIMM Agendo credentials**. | * **Login** to Windows with your **GIMM Agendo credentials**. | ||
| - | * Wait for the **button light** in the front to **turn green** (the system will pop out the plate drawer). | + | * Wait for the **button light** in the front to **turn green** (the system will pop out the plate drawer as well). |
| - | {{cytation_turnon_3.png?|}} | + | {{cytation_turnon_3.png?200|}} |
| * Open the **Gen 5** software. | * Open the **Gen 5** software. | ||
| + | |||
| + | |||
| + | ===== Microscope Turn Off Procedure ===== | ||
| + | |||
| + | * Turn off the microscope using the **switch** at the **front of the system**. | ||
| + | {{cytation_turnon_1.png?200|}} | ||
| + | * **Sign out** of the computer. | ||
| + | |||
| + | * **If you used the gas controller** | ||
| + | * Turn gas controller OFF | ||
| + | {{cytation_gas_1.png?200|}} | ||
| + | * Close all wall taps. | ||
| + | {{cytation_gas_3.png?200|}} | ||
| ---- | ---- | ||
| [[resources|Back to the Equipment page]] | [[resources|Back to the Equipment page]] | ||
agilent_cytation_5.1736348853.txt.gz · Last modified: 2025/01/08 16:07 by bioimaging
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