agilent_cytation_5
Differences
This shows you the differences between two versions of the page.
| Both sides previous revision Previous revision Next revision | Previous revision | ||
|
agilent_cytation_5 [2025/01/08 15:44] bioimaging |
agilent_cytation_5 [2025/03/10 16:45] (current) bioimaging |
||
|---|---|---|---|
| Line 12: | Line 12: | ||
| <b>Software</b>: Gen 5 Image Prime <br> | <b>Software</b>: Gen 5 Image Prime <br> | ||
| <b>Year</b>: 2024 <br> | <b>Year</b>: 2024 <br> | ||
| - | <b>SN</b>: <br> | + | <b>SN</b>: JDGD GHWD HGWM KH<br> |
|   <br> |   <br> | ||
| Data will be deleted after: <b>1 month</b> | Data will be deleted after: <b>1 month</b> | ||
| Line 37: | Line 37: | ||
| ===== Filtersets available ===== | ===== Filtersets available ===== | ||
| - | ^ Filterset ^ Filterset Reference ^ LED wavelength (nm) ^ LED reference ^ Excitation (nm) ^ Dichroic (nm) ^ Emission (nm) ^ Default Config ^ | + | ^ Default Config ^ Filterset ^ Filterset Reference ^ LED wavelength (nm) ^ LED reference ^ Excitation (nm) ^ Dichroic (nm) ^ Emission (nm) ^ |
| - | | Laser Autofocus | [[https://www.agilent.com/store/productDetail.jsp?catalogId=1225010&catId=SubCat2ECS_2041516|1225010]] | - | - | - | - | - | Pos 1 | | + | | Pos 1 | Laser Autofocus | [[https://www.agilent.com/store/productDetail.jsp?catalogId=1225010&catId=SubCat2ECS_2041516|1225010]] | - | - | - | - | - | |
| - | | DAPI | [[https://www.agilent.com/store/en_US/Prod-1225100/1225100|1225100]] | 365 | 1225007 | 377/50 | 409 | 447/60 | Pos 2 | | + | | Pos 2 | DAPI | [[https://www.agilent.com/store/en_US/Prod-1225100/1225100|1225100]] | 365 | 1225007 | 377/50 | 409 | 447/60 | |
| - | | GFP | [[https://www.agilent.com/store/en_US/Prod-1225101/1225101|1225101]] | 465 | 1225001 | 469/35 | 497 | 525/39 | - | | + | | - | GFP | [[https://www.agilent.com/store/en_US/Prod-1225101/1225101|1225101]] | 465 | 1225001 | 469/35 | 497 | 525/39 | |
| - | | Propidium Iodide | [[https://www.agilent.com/store/en_US/Prod-1225111/1225111|1225111]] | 523 | 1225003 | 531/40 | 605 | 647/57 | Pos 3 | | + | | Pos 3 | Propidium Iodide | [[https://www.agilent.com/store/en_US/Prod-1225111/1225111|1225111]] | 523 | 1225003 | 531/40 | 605 | 647/57 | |
| - | | Texas Red | [[https://www.agilent.com/store/en_US/Prod-1225102/1225102|1225102]] | 590 | 1225002 | 586/15 | 605 | 647/57 | - | | + | | - | Texas Red | [[https://www.agilent.com/store/en_US/Prod-1225102/1225102|1225102]] | 590 | 1225002 | 586/15 | 605 | 647/57 | |
| - | | Cy5 | [[https://www.agilent.com/store/en_US/Prod-1225105/|1225105]] | 623 | 1225005 | 628/40 | 660 | 685/40 | Pos 4 | | + | | Pos 4 | Cy5 | [[https://www.agilent.com/store/en_US/Prod-1225105/|1225105]] | 623 | 1225005 | 628/40 | 660 | 685/40 | |
| Line 59: | Line 59: | ||
| - | ===== Microscope Turn On Procedure ===== | + | ===== Pixel Size ===== |
| - | * Turn on the microscope using the **switch** at the **front of the system**. | + | Images coming from the Cytation 5 are not calibrated, even though the system has that information available. In the images' metadata you can find the PixelWidth and the ImageWidthMicrons, and by dividing the latter for the first, you get the Pixel Size to calibrate your images. |
| + | You can find this information below, per objective and Field Of View (FOV): | ||
| - | * **If you require CO2/O2 control**, turn the **atmosphere controller ON** | + | ^ Objective ^ FOV ^ Image Size (pixels) ^ Image Size (microns) ^ Pixel Size (microns) ^ |
| + | | 4x | Full | 1992 x 1992 | 3474 x 3474 | ~1.7440 x ~1.7440 | | ||
| + | | 4x | 75% | 1496 x 1496 | 2609 x 2609 | ~1.7440 x ~1.7440 | | ||
| + | | 4x | Standard | 1224 x 904 | 2135 x 1576 | ~1.7443 x ~1.7434 | | ||
| + | | 20x | Full | 1992 x 1992 | 694 x 694 | ~0.3484 x ~0.3484 | | ||
| + | | 20x | 75% | 1496 x 1496 | 521 x 521 | ~0.3483 x ~0.3483 | | ||
| + | | 20x | Standard | 1224 x 904 | 427 x 315 | ~0.3489 x ~0.3485 | | ||
| + | | 40x | Full | 1992 x 1992 | 347 x 347 | ~0.1742 x ~0.1742 | | ||
| + | | 40x | 75% | 1496 x 1496 | 260 x 260 | ~0.1738 x ~0.1738 | | ||
| + | | 40x | Standard | 1224 x 904 | 213 x 157 | ~0.1740 x ~0.1737 | | ||
| - | * Confirm the **CO2/O2 switch is ON**, set to the desired percentage, and **open the CO2/N2 tap** on the wall. | ||
| - | * Turn the **computer** on. | + | ===== CO2 and/or O2 Control Procedure ===== |
| - | * **Login** to Windows with your **GIMM Agendo credentials**. | + | * **If you require CO2 and/or O2 control**, turn the **atmosphere controller ON**. |
| + | {{cytation_gas_1.png?200|}} | ||
| + | * Confirm the **CO2 and/or O2 switch is ON**, and set to the desired percentage. | ||
| + | {{cytation_gas_2.png?600|}} | ||
| + | * **Open the CO2 and/or N2 tap** on the wall. | ||
| + | {{cytation_gas_3.png?200|}} | ||
| - | * Wait for the **button light** in the front to **turn green**. | ||
| + | ===== Microscope Turn On Procedure ===== | ||
| + | |||
| + | * Turn on the microscope using the **switch** at the **front of the system**. | ||
| + | {{cytation_turnon_1.png?200|}} | ||
| + | * Turn the **computer** on. | ||
| + | {{cytation_turnon_2.png?200|}} | ||
| + | * **Login** to Windows with your **GIMM Agendo credentials**. | ||
| + | |||
| + | * Wait for the **button light** in the front to **turn green** (the system will pop out the plate drawer as well). | ||
| + | {{cytation_turnon_3.png?200|}} | ||
| * Open the **Gen 5** software. | * Open the **Gen 5** software. | ||
| + | |||
| + | ===== Microscope Turn Off Procedure ===== | ||
| + | |||
| + | * Turn off the microscope using the **switch** at the **front of the system**. | ||
| + | {{cytation_turnon_1.png?200|}} | ||
| + | * **Sign out** of the computer. | ||
| + | |||
| + | * **If you used the gas controller** | ||
| + | * Turn gas controller OFF | ||
| + | {{cytation_gas_1.png?200|}} | ||
| + | * Close all wall taps. | ||
| + | {{cytation_gas_3.png?200|}} | ||
| ---- | ---- | ||
| [[resources|Back to the Equipment page]] | [[resources|Back to the Equipment page]] | ||
agilent_cytation_5.1736347498.txt.gz · Last modified: 2025/01/08 15:44 by bioimaging
Page Tools
