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3i_marianas_sdc [2025/12/12 15:05] bioimaging |
3i_marianas_sdc [2026/09/08 01:06] (current) bioimaging [LASERs] |
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| - | <img src="/facility/bioimaging/lib/exe/fetch.php?media=lisbon_3i_marianas_sdc_name_gimm_2024.png"> | + | <img src="/facility/bioimaging/lib/exe/fetch.php?media=oeiras_3i_marianas_sdc_tirf_name.png"> |
| <table style="border:0px solid white;"> | <table style="border:0px solid white;"> | ||
| <tr style="border:0px solid white;"> | <tr style="border:0px solid white;"> | ||
| <td style="border:0px solid white;"> | <td style="border:0px solid white;"> | ||
| - | <a href="/facility/bioimaging/lib/exe/fetch.php?media=3i_marianas_sdc_wiki_2024.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=3i_marianas_sdc_wiki_2024.png" width=300></a> | + | <a href="/facility/bioimaging/lib/exe/fetch.php?media=oeiras_marianas_tirf.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=oeiras_marianas_tirf.jpg" width=300></a> |
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| - | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=3i_marianas_sdc_map_new.png">[Lisbon] Room P2-A-22</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47217) <br> | + | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=bartolomeu_dias_wing_2025_-_3i_marianas_sdc_tirf_.png">[Oeiras] Room 0B03</a><br> |
| <b>Manufacturer</b>: <a href="https://www.intelligent-imaging.com/" target="_blank">Intelligent Imaging Innovations</a><br> | <b>Manufacturer</b>: <a href="https://www.intelligent-imaging.com/" target="_blank">Intelligent Imaging Innovations</a><br> | ||
| <b>Model</b>: <a href="https://www.intelligent-imaging.com/marianas" target="_blank">Marianas SDC</a><br> | <b>Model</b>: <a href="https://www.intelligent-imaging.com/marianas" target="_blank">Marianas SDC</a><br> | ||
| - | <b>Nickname</b>: "Marianas"<br> | + | <b>Nickname</b>: "Marianas TIRF"<br> |
| - | <b>Software</b>: SlideBook 2024<br> | + | <b>Software</b>: SlideBook 2026.1<br> |
| - | <b>Year</b>: 2012 <br> | + | <b>Year</b>: 2012<br> |
| - | <b>SN</b>: 1648 <br> | + | <b>SN</b>: CS-01648<br> |
|   <br> |   <br> | ||
| Data will be deleted after: <b>15 days</b> | Data will be deleted after: <b>15 days</b> | ||
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| ===== System overview ===== | ===== System overview ===== | ||
| - | {{ :yokogawa_sd.png?350|}} The 3i Marianas SDC //spinning disk confocal microscope// is a fast imaging system which provides a trade-off between confocality, resolution and speed. It is an inverted microscope ideal for live cell applications which require fast acquisition speeds rather than high resolution images. The scanning unit achieves confocality by directing light through a spinning disk with many small pinholes. Images are then acquired with a sensitive EMCCD which allows for very small exposure times but is limited in resolution to 512x512 pixels. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions. The system is also equipped with a large cage incubator and a stage top incubator for temperature control and CO2 supply. | + | {{ :yokogawa_sd.png?350|}} The 3i Marianas SDC //spinning disk confocal microscope// is a fast imaging system which provides a trade-off between confocality, resolution and speed. It is an inverted microscope ideal for live cell applications which require fast acquisition speeds rather than high resolution images. The scanning unit achieves confocality by directing light through a spinning disk with many small pinholes. Images are then acquired with a sensitive EMCCD which allows for very small exposure times but is limited in resolution to 512x512 pixels. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions, with a large cage incubator and a stage top incubator for temperature control and CO2 supply allowing for live-cell imaging. |
| + | The system is also equipped with a Total Internal Reflection Fluorescence (TIRF) and photomanipulation module, providing motorized TIRF and HILO illumination for selective excitation within ~100–150 nm of the coverslip and integrated photomanipulation capabilities, including FRAP, photoactivation and photoconversion, ideal for studying membrane dynamics, receptor and vesicle movement. | ||
| * **Microscope**: [[https://www.zeiss.com/microscopy/en/products/light-microscopes/widefield-microscopes/axio-observer-for-life-science-research.html|Zeiss Axio Observer]] | * **Microscope**: [[https://www.zeiss.com/microscopy/en/products/light-microscopes/widefield-microscopes/axio-observer-for-life-science-research.html|Zeiss Axio Observer]] | ||
| * **Confocal scanner**: [[https://www.yokogawa.com/eu/solutions/products-and-services/life-science/spinning-disk-confocal/csu-x1-confocal-scanner-unit/|Yokogawa CSU-X1]] | * **Confocal scanner**: [[https://www.yokogawa.com/eu/solutions/products-and-services/life-science/spinning-disk-confocal/csu-x1-confocal-scanner-unit/|Yokogawa CSU-X1]] | ||
| * **Camera**: [[https://www.photometrics.com/wp-content/uploads/2019/10/Evolve512-Datasheet.pdf|Photometrics Evolve 512 EMCCD]] | * **Camera**: [[https://www.photometrics.com/wp-content/uploads/2019/10/Evolve512-Datasheet.pdf|Photometrics Evolve 512 EMCCD]] | ||
| + | * **Features** | ||
| + | * | [[https://www.intelligent-imaging.com/tirf|Vector3 TIRF and Photomanipulation]] | ||
| {{:warning.png?25|}} Data files older than **15 days** will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link. | {{:warning.png?25|}} Data files older than **15 days** will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link. | ||
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| | Blue | <fc #00f7ff>488 nm</fc> | 100 mW | | | Blue | <fc #00f7ff>488 nm</fc> | 100 mW | | ||
| | Yellow-Green | <fc #c6ff00>561 nm</fc> | 100 mW | | | Yellow-Green | <fc #c6ff00>561 nm</fc> | 100 mW | | ||
| - | | Red | <fc #ff4200>640 nm</fc> | 100 mW | | + | | Red | <fc #ff4200>637 nm</fc> | 140 mW | |
| ==== Objectives ==== | ==== Objectives ==== | ||
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| | <fc #01b3f5>40x</fc> | Plan-Apochromat Corr | Air | 0.95 | 0.25 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420660-9970-000/Objective-Plan-Apochromat-40x-0.95-Corr-M27|420660-9970-000]] | | | <fc #01b3f5>40x</fc> | Plan-Apochromat Corr | Air | 0.95 | 0.25 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420660-9970-000/Objective-Plan-Apochromat-40x-0.95-Corr-M27|420660-9970-000]] | | ||
| - | ==== Emission Filters ==== | + | ==== Emission Filters CSU X1 ==== |
| - | ^ Setting ^ Transmission ^ | + | |
| - | | 1: 445 | <fc #0041ff>422-477 nm</fc> | | + | ^ Position ^ Setting ^ Transmission ^ |
| - | | 2: 525 | <fc #4aff00>510-540 nm</fc> | | + | | 1 | 445 | <fc #0041ff>422-477 nm</fc> | |
| - | | 3: 617 | <fc #ff8200>580-653 nm</fc> | | + | | 2 | 525 | <fc #4aff00>510-540 nm</fc> | |
| - | | 4: 692 | <fc #ff0000>672-712 nm</fc> | | + | | 3 | 617 | <fc #ff8200>580-653 nm</fc> | |
| - | | 5: QUAD | <fc #0041ff>440</fc>+<fc #4aff00>521</fc>+<fc #ffa600>607</fc>+<fc #ff0000>700</fc> nm | | + | | 4 | 692 | <fc #ff0000>672-712 nm</fc> | |
| - | | 6: Block | No Transmission | | + | | 5 | QUAD | <fc #0041ff>440</fc>+<fc #4aff00>521</fc>+<fc #ffa600>607</fc>+<fc #ff0000>700</fc> nm | |
| - | | 7: Block | No Transmission | | + | | 6 | Block | No Transmission | |
| - | | 8: Block | No Transmission | | + | | 7 | Block | No Transmission | |
| - | | 9: Empty | Empty | | + | | 8 | Block | No Transmission | |
| - | | 10: 445 | <fc #0041ff>422-477 nm</fc> | | + | | 9 | Empty | Empty | |
| + | | 10 | 445 | <fc #0041ff>422-477 nm</fc> | | ||
| ==== Camera ==== | ==== Camera ==== | ||
3i_marianas_sdc.1765548343.txt.gz · Last modified: 2025/12/12 15:05 by bioimaging
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