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3i_marianas_sdc [2025/12/12 14:39] bioimaging [Objectives] |
3i_marianas_sdc [2026/06/05 16:46] (current) bioimaging |
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| <b>Model</b>: <a href="https://www.intelligent-imaging.com/marianas" target="_blank">Marianas SDC</a><br> | <b>Model</b>: <a href="https://www.intelligent-imaging.com/marianas" target="_blank">Marianas SDC</a><br> | ||
| <b>Nickname</b>: "Marianas"<br> | <b>Nickname</b>: "Marianas"<br> | ||
| - | <b>Software</b>: SlideBook 2024<br> | + | <b>Software</b>: SlideBook 2026.0<br> |
| - | <b>Year</b>: 2012 <br> | + | <b>Year</b>: 2012<br> |
| - | <b>SN</b>: 1648 <br> | + | <b>SN</b>: CS-01648<br> |
|   <br> |   <br> | ||
| Data will be deleted after: <b>15 days</b> | Data will be deleted after: <b>15 days</b> | ||
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| ===== System overview ===== | ===== System overview ===== | ||
| - | |||
| {{ :yokogawa_sd.png?350|}} The 3i Marianas SDC //spinning disk confocal microscope// is a fast imaging system which provides a trade-off between confocality, resolution and speed. It is an inverted microscope ideal for live cell applications which require fast acquisition speeds rather than high resolution images. The scanning unit achieves confocality by directing light through a spinning disk with many small pinholes. Images are then acquired with a sensitive EMCCD which allows for very small exposure times but is limited in resolution to 512x512 pixels. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions. The system is also equipped with a large cage incubator and a stage top incubator for temperature control and CO2 supply. | {{ :yokogawa_sd.png?350|}} The 3i Marianas SDC //spinning disk confocal microscope// is a fast imaging system which provides a trade-off between confocality, resolution and speed. It is an inverted microscope ideal for live cell applications which require fast acquisition speeds rather than high resolution images. The scanning unit achieves confocality by directing light through a spinning disk with many small pinholes. Images are then acquired with a sensitive EMCCD which allows for very small exposure times but is limited in resolution to 512x512 pixels. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions. The system is also equipped with a large cage incubator and a stage top incubator for temperature control and CO2 supply. | ||
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| | <fc #01b3f5>40x</fc> | Plan-Apochromat Corr | Air | 0.95 | 0.25 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420660-9970-000/Objective-Plan-Apochromat-40x-0.95-Corr-M27|420660-9970-000]] | | | <fc #01b3f5>40x</fc> | Plan-Apochromat Corr | Air | 0.95 | 0.25 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420660-9970-000/Objective-Plan-Apochromat-40x-0.95-Corr-M27|420660-9970-000]] | | ||
| - | ==== Emission Filters ==== | + | ==== Emission Filters CSU X1 ==== |
| - | ^ Setting ^ Transmission ^ | + | |
| - | | 1: 445 | 422-477 nm | | + | ^ Position ^ Setting ^ Transmission ^ |
| - | | 2: 525 | 510-540 nm | | + | | 1 | 445 | <fc #0041ff>422-477 nm</fc> | |
| - | | 3: 617 | 580-653 nm | | + | | 2 | 525 | <fc #4aff00>510-540 nm</fc> | |
| - | | 4: 692 | 672-712 nm | | + | | 3 | 617 | <fc #ff8200>580-653 nm</fc> | |
| - | | 5: QUAD | 440+521+607+700 nm | | + | | 4 | 692 | <fc #ff0000>672-712 nm</fc> | |
| - | | 6: Block | No Transmission | | + | | 5 | QUAD | <fc #0041ff>440</fc>+<fc #4aff00>521</fc>+<fc #ffa600>607</fc>+<fc #ff0000>700</fc> nm | |
| - | | 7: Block | No Transmission | | + | | 6 | Block | No Transmission | |
| - | | 8: Block | No Transmission | | + | | 7 | Block | No Transmission | |
| - | | 9: Empty | Empty | | + | | 8 | Block | No Transmission | |
| - | | 10: 445 | 422-477 nm | | + | | 9 | Empty | Empty | |
| + | | 10 | 445 | <fc #0041ff>422-477 nm</fc> | | ||
| ==== Camera ==== | ==== Camera ==== | ||
3i_marianas_sdc.1765546770.txt.gz · Last modified: 2025/12/12 14:39 by bioimaging
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