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3i_marianas_sdc
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3i_marianas_sdc [2025/07/19 21:34] bioimaging |
3i_marianas_sdc [2026/03/04 15:33] (current) bioimaging |
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| <b>Model</b>: <a href="https://www.intelligent-imaging.com/marianas" target="_blank">Marianas SDC</a><br> | <b>Model</b>: <a href="https://www.intelligent-imaging.com/marianas" target="_blank">Marianas SDC</a><br> | ||
| <b>Nickname</b>: "Marianas"<br> | <b>Nickname</b>: "Marianas"<br> | ||
| - | <b>Software</b>: SlideBook 2024<br> | + | <b>Software</b>: SlideBook 2025.2.0<br> |
| <b>Year</b>: 2012 <br> | <b>Year</b>: 2012 <br> | ||
| <b>SN</b>: 1648 <br> | <b>SN</b>: 1648 <br> | ||
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| ===== System overview ===== | ===== System overview ===== | ||
| - | |||
| {{ :yokogawa_sd.png?350|}} The 3i Marianas SDC //spinning disk confocal microscope// is a fast imaging system which provides a trade-off between confocality, resolution and speed. It is an inverted microscope ideal for live cell applications which require fast acquisition speeds rather than high resolution images. The scanning unit achieves confocality by directing light through a spinning disk with many small pinholes. Images are then acquired with a sensitive EMCCD which allows for very small exposure times but is limited in resolution to 512x512 pixels. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions. The system is also equipped with a large cage incubator and a stage top incubator for temperature control and CO2 supply. | {{ :yokogawa_sd.png?350|}} The 3i Marianas SDC //spinning disk confocal microscope// is a fast imaging system which provides a trade-off between confocality, resolution and speed. It is an inverted microscope ideal for live cell applications which require fast acquisition speeds rather than high resolution images. The scanning unit achieves confocality by directing light through a spinning disk with many small pinholes. Images are then acquired with a sensitive EMCCD which allows for very small exposure times but is limited in resolution to 512x512 pixels. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions. The system is also equipped with a large cage incubator and a stage top incubator for temperature control and CO2 supply. | ||
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| ==== LASERs ==== | ==== LASERs ==== | ||
| - | ^ Laser Unit ^ Wavelength ^ Maximum Power ^ | + | |
| - | | Laserstack 405 | 405 nm | 100 mW | | + | ^ LASER ^ Wavelength ^ Maximum Power ^ |
| - | | Laserstack 488 | 488 nm | 100 mW | | + | | Violet | <fc #a600ff>405 nm</fc> | 100 mW | |
| - | | Laserstack 561 | 561 nm | 100 mW | | + | | Blue | <fc #00f7ff>488 nm</fc> | 100 mW | |
| - | | Laserstack 640 | 640 nm | 100 mW | | + | | Yellow-Green | <fc #c6ff00>561 nm</fc> | 100 mW | |
| + | | Red | <fc #ff4200>640 nm</fc> | 100 mW | | ||
| ==== Objectives ==== | ==== Objectives ==== | ||
| ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ||
| - | | 10x | EC Plan-Neofluar | Air | 0.30 | 5.50 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420340-9900-000/Objective-EC-Plan-Neofluar-10x-0.30-M27|420340-9900-000]] | | + | | <fc #fef042>10x</fc> | EC Plan-Neofluar | Air | 0.30 | 5.50 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420340-9900-000/Objective-EC-Plan-Neofluar-10x-0.30-M27|420340-9900-000]] | |
| - | | 20x | Plan-Apochromat | Air | 0.80 | 0.55 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420650-9901-000/Objective-Plan-Apochromat-20x-0.8-M27|420650-9901-000]] | | + | | <fc #0aa05c>20x</fc> | Plan-Apochromat | Air | 0.80 | 0.55 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420650-9901-000/Objective-Plan-Apochromat-20x-0.8-M27|420650-9901-000]] | |
| - | | 40x | LD C-Apochromat Corr | Water | 1.10 | 0.62 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/421867-9970-000/Objective-LD-C-Apochromat-40x-1.1-W-Corr-M27|421867-9970-000]] | | + | | <fc #01b3f5>40x</fc> | LD C-Apochromat Corr | Water | 1.10 | 0.62 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/421867-9970-000/Objective-LD-C-Apochromat-40x-1.1-W-Corr-M27|421867-9970-000]] | |
| - | | 63x | Plan-Apochromat | Oil | 1.40 | 0.19 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420780-9900-000/Objective-Plan-Apochromat-63x-1.4-Oil-M27|420780-9900-000]] | | + | | <fc #2c80c0>63x</fc> | Plan-Apochromat | Oil | 1.40 | 0.19 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420780-9900-000/Objective-Plan-Apochromat-63x-1.4-Oil-M27|420780-9900-000]] | |
| | 100x | α Plan-Apochromat DIC | Oil | 1.46 | 0.11 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420792-9800-000/Objective-alpha-Plan-Apochromat-100x-1.46-Oil-DIC-M27|420792-9800-000]] | | | 100x | α Plan-Apochromat DIC | Oil | 1.46 | 0.11 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420792-9800-000/Objective-alpha-Plan-Apochromat-100x-1.46-Oil-DIC-M27|420792-9800-000]] | | ||
| **Upon request:** | **Upon request:** | ||
| ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ||
| - | | 25x | LCI Plan-Neofluar Corr DIC | Oil/Glyc/W | 0.80 | 0.21 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420852-9972-000/Objective-LCI-Plan-Neofluar-25x-0.8-Imm-Corr-DIC-M27|420852-9972-000]] | | + | | <fc #0aa05c>25x</fc> | LCI Plan-Neofluar Corr DIC | Oil/Glyc/W | 0.80 | 0.21 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420852-9972-000/Objective-LCI-Plan-Neofluar-25x-0.8-Imm-Corr-DIC-M27|420852-9972-000]] | |
| - | | 40x | Plan-Apochromat Corr | Air | 0.95 | 0.25 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420660-9970-000/Objective-Plan-Apochromat-40x-0.95-Corr-M27|420660-9970-000]] | | + | | <fc #01b3f5>40x</fc> | Plan-Apochromat Corr | Air | 0.95 | 0.25 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420660-9970-000/Objective-Plan-Apochromat-40x-0.95-Corr-M27|420660-9970-000]] | |
| - | ==== Emission Filters ==== | + | ==== Emission Filters CSU X1 ==== |
| - | ^ Setting ^ Transmission ^ | + | |
| - | | 1: 445 | 422-477 nm | | + | ^ Position ^ Setting ^ Transmission ^ |
| - | | 2: 525 | 510-540 nm | | + | | 1 | 445 | <fc #0041ff>422-477 nm</fc> | |
| - | | 3: 617 | 580-653 nm | | + | | 2 | 525 | <fc #4aff00>510-540 nm</fc> | |
| - | | 4: 692 | 672-712 nm | | + | | 3 | 617 | <fc #ff8200>580-653 nm</fc> | |
| - | | 5: QUAD | 440+521+607+700 nm | | + | | 4 | 692 | <fc #ff0000>672-712 nm</fc> | |
| - | | 6: Block | No Transmission | | + | | 5 | QUAD | <fc #0041ff>440</fc>+<fc #4aff00>521</fc>+<fc #ffa600>607</fc>+<fc #ff0000>700</fc> nm | |
| - | | 7: Block | No Transmission | | + | | 6 | Block | No Transmission | |
| - | | 8: Block | No Transmission | | + | | 7 | Block | No Transmission | |
| - | | 9: Empty | Empty | | + | | 8 | Block | No Transmission | |
| - | | 10: 445 | 422-477 nm | | + | | 9 | Empty | Empty | |
| + | | 10 | 445 | <fc #0041ff>422-477 nm</fc> | | ||
| ==== Camera ==== | ==== Camera ==== | ||
| Line 86: | Line 87: | ||
| * Turn on the lasers you need to use (using the key and the power switches) | * Turn on the lasers you need to use (using the key and the power switches) | ||
| {{::marianas_power7.png?160|}} | {{::marianas_power7.png?160|}} | ||
| - | * Turn on the computer (if needed) | + | * Turn on the computer |
| * Login in to Windows with your Agendo credentials | * Login in to Windows with your Agendo credentials | ||
| * Wait 2 min to allow for system components detection | * Wait 2 min to allow for system components detection | ||
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| Else: | Else: | ||
| * Exit the **SlideBook** software | * Exit the **SlideBook** software | ||
| - | * Log off the computer | + | * Shutdown the computer |
| * Turn off incubation in the touchscreen (if used) | * Turn off incubation in the touchscreen (if used) | ||
| * Turn off CO2 (if used) | * Turn off CO2 (if used) | ||
3i_marianas_sdc.1752953643.txt.gz · Last modified: 2025/07/19 21:34 by bioimaging
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