3i_marianas_sdc
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3i_marianas_sdc [2023/03/27 12:51] bioimaging |
3i_marianas_sdc [2025/01/14 16:35] (current) bioimaging [System overview] |
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| - | <img src="/facility/bioimaging/lib/exe/fetch.php?media=3i_marianas_sdc_name_new.png"> | + | <img src="/facility/bioimaging/lib/exe/fetch.php?media=lisbon_3i_marianas_sdc_name_gimm_2024.png"> |
| <table style="border:0px solid white;"> | <table style="border:0px solid white;"> | ||
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| - | <a href="/facility/bioimaging/lib/exe/fetch.php?media=3i_marianas_sdc_wiki_2022.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=3i_marianas_sdc_wiki_2022.png" width=300></a> | + | <a href="/facility/bioimaging/lib/exe/fetch.php?media=3i_marianas_sdc_wiki_2024.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=3i_marianas_sdc_wiki_2024.png" width=300></a> |
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| - | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=3i_marianas_sdc_map_new.png">Room P2-A-22</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47217) <br> | + | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=3i_marianas_sdc_map_new.png">[Lisbon] Room P2-A-22</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47217) <br> |
| <b>Manufacturer</b>: <a href="https://www.intelligent-imaging.com/" target="_blank">Intelligent Imaging Innovations</a><br> | <b>Manufacturer</b>: <a href="https://www.intelligent-imaging.com/" target="_blank">Intelligent Imaging Innovations</a><br> | ||
| - | <b>Model</b>: <a href="https://www.intelligent-imaging.com/marianas" target="_blank">Marianas</a><br> | + | <b>Model</b>: <a href="https://www.intelligent-imaging.com/marianas" target="_blank">Marianas SDC</a><br> |
| <b>Nickname</b>: "Marianas"<br> | <b>Nickname</b>: "Marianas"<br> | ||
| - | <b>Software</b>: SlideBook 6.0.22<br> | + | <b>Software</b>: SlideBook 2024<br> |
| <b>Year</b>: 2012 <br> | <b>Year</b>: 2012 <br> | ||
| <b>SN</b>: 1648 <br> | <b>SN</b>: 1648 <br> | ||
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| - | {{ :yokogawa_sd.png?350|}} The 3i Marianas SDC //spinning disk confocal microscope// is a fast imaging system which provides a trade-off between confocality, resolution and speed. It is an inverted microscope ideal for live cell applications which require fast acquisition speeds rather than high resolution images. The scanning unit achieves confocality by directing light through a spinning disk with many small pinholes. Images are then acquired with a sensitive EMCCD which allows for very small exposure times but is limited in resolution to 512x512 pixels. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions. The system is also equipped with a large cage incubator and a stage top incubator for temperature control and CO2 supply. With this system you can perform optical sectioning of fluorescent samples which are too thick for a widefield system such as the [[zeiss_cell_observer|Zeiss Cell Observer]]. Even though its resolution is not as high as a //point-scanning confocal system// such as the [[zeiss_lsm_710|Zeiss LSM 710]], it is much faster. You can also check the [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]] if you need a spinning disk with Definite Focus 2 for large tiling or long time-lapse acquisitions. If your personal computer cannot handle all the data you collected, check out the [[colossus|Colossus]]. | + | {{ :yokogawa_sd.png?350|}} The 3i Marianas SDC //spinning disk confocal microscope// is a fast imaging system which provides a trade-off between confocality, resolution and speed. It is an inverted microscope ideal for live cell applications which require fast acquisition speeds rather than high resolution images. The scanning unit achieves confocality by directing light through a spinning disk with many small pinholes. Images are then acquired with a sensitive EMCCD which allows for very small exposure times but is limited in resolution to 512x512 pixels. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions. The system is also equipped with a large cage incubator and a stage top incubator for temperature control and CO2 supply. |
| - | * **Microscope**: [[http://www.zeiss.com/microscopy/en_de/products/light-microscopes/axio-observer-for-biology.html|Zeiss Axio Observer]] | + | * **Microscope**: [[https://www.zeiss.com/microscopy/en/products/light-microscopes/widefield-microscopes/axio-observer-for-life-science-research.html|Zeiss Axio Observer]] |
| - | * **Confocal scanner**: [[https://www.yokogawa.com/eu/solutions/products-platforms/life-science/spinning-disk-confocal/csu-x1-confocal-scanner-unit/|Yokogawa CSU-X1]] | + | * **Confocal scanner**: [[https://www.yokogawa.com/eu/solutions/products-and-services/life-science/spinning-disk-confocal/csu-x1-confocal-scanner-unit/|Yokogawa CSU-X1]] |
| * **Camera**: [[https://www.photometrics.com/wp-content/uploads/2019/10/Evolve512-Datasheet.pdf|Photometrics Evolve 512 EMCCD]] | * **Camera**: [[https://www.photometrics.com/wp-content/uploads/2019/10/Evolve512-Datasheet.pdf|Photometrics Evolve 512 EMCCD]] | ||
| - | {{:warning.png?25|}} Data files older than **15 days** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | + | {{:warning.png?25|}} Data files older than **15 days** will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link. |
| ===== System components ===== | ===== System components ===== | ||
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| | 40x | LD C-Apochromat Corr | Water | 1.10 | 0.62 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/421867-9970-000/Objective-LD-C-Apochromat-40x-1.1-W-Corr-M27|421867-9970-000]] | | | 40x | LD C-Apochromat Corr | Water | 1.10 | 0.62 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/421867-9970-000/Objective-LD-C-Apochromat-40x-1.1-W-Corr-M27|421867-9970-000]] | | ||
| | 63x | Plan-Apochromat | Oil | 1.40 | 0.19 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420780-9900-000/Objective-Plan-Apochromat-63x-1.4-Oil-M27|420780-9900-000]] | | | 63x | Plan-Apochromat | Oil | 1.40 | 0.19 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420780-9900-000/Objective-Plan-Apochromat-63x-1.4-Oil-M27|420780-9900-000]] | | ||
| - | | 100x | Plan-Apochromat | Oil | 1.40 | 0.17 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420790-9901-000/Objective-Plan-Apochromat-100x-1.40-Oil-M27|420790-9901-000]] | | + | | 100x | α Plan-Apochromat DIC | Oil | 1.46 | 0.11 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420792-9800-000/Objective-alpha-Plan-Apochromat-100x-1.46-Oil-DIC-M27|420792-9800-000]] | |
| **Upon request:** | **Upon request:** | ||
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| * Turn on the power switch distributor (green) and the component power switches (red) **one at a time** from left to right | * Turn on the power switch distributor (green) and the component power switches (red) **one at a time** from left to right | ||
| {{marianas_power3.jpg?|}} | {{marianas_power3.jpg?|}} | ||
| - | * Turn on the secondary power supply switch (socket outlet underneath the touchscreen) | + | * Turn on the secondary power supply switch (socket outlet below the touchscreen) |
| {{::marianas_power2.png|}} | {{::marianas_power2.png|}} | ||
| * Turn on incubation and CO2 in the touchscreen (if needed) | * Turn on incubation and CO2 in the touchscreen (if needed) | ||
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| * Turn off CO2 (if used) | * Turn off CO2 (if used) | ||
| * Switch all lasers off (first on the switch and finally by turning the key) | * Switch all lasers off (first on the switch and finally by turning the key) | ||
| - | * Turn off the fluorescent lamp | + | * Turn off the secondary power supply switch (white socket outlet below the touchscreen) |
| - | * Turn off the secondary power supply switch (white socket outlet underneath the touchscreen) | + | |
| * Turn off the component power switches (red) **one at a time** from right to left and the power switch distributor (green) | * Turn off the component power switches (red) **one at a time** from right to left and the power switch distributor (green) | ||
| + | * Turn off the fluorescent lamp | ||
| ---- | ---- | ||
| [[resources|Back to the Equipment page]] | [[resources|Back to the Equipment page]] | ||
3i_marianas_sdc.1679914311.txt.gz · Last modified: 2023/03/27 12:51 by bioimaging
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