zeiss_lumar_v12
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zeiss_lumar_v12 [2016/04/09 03:56] bioimaging created |
zeiss_lumar_v12 [2016/06/01 13:19] bioimaging |
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| - | → <a href="https://imm.medicina.ulisboa.pt/intranet/booking/month.php?user=ZEISSLUMA&rid=58"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> Zeiss Lumar V12 Booking</a> | + | → <a href="https://imm.medicina.ulisboa.pt/intranet/booking/58"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> Zeiss Lumar V12 Booking</a> |
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| → <a href="/facility/bioimaging/doku.php?id=zeiss_lumar_v12_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> Zeiss Lumar V12 Usage Statistics</a> | → <a href="/facility/bioimaging/doku.php?id=zeiss_lumar_v12_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> Zeiss Lumar V12 Usage Statistics</a> | ||
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| + | ===== Microscope overview ===== | ||
| + | The Zeiss Lumar V12 is a //fluorescence stereo microscope// suitable for high resolution 3D analysis of large specimens in fluorescent and transmitted light. The system is equipped with fluorescence filters for GFP, Cy3 and Cy5.5 and a sensitive cooled CCD camera ([[http://www.hamamatsu.com/jp/en/community/life_science_camera/product/search/C10600-10B/index.html|ORCA R2]]) that allows you to capture weak fluorescent signals and minimize photobleaching in light sensitive samples. With the Zeiss Lumar V12 you can perform multi-channel, Z-stack and time-lapse image acquisition of your samples, using two different objectives with different working distances and magnification values. If you need higher magnifications, check out the [[leica_dm5000b|Leica DM5000B]] or the [[zeiss_axiovert_200m|Zeiss Axiovert 200M]]. | ||
| + | |||
| + | ===== System components ===== | ||
| + | |||
| + | ==== Filtersets ==== | ||
| + | |||
| + | ^ Position ^ Filterset ^ Reference ^ Excitation ^ Emission ^ | ||
| + | | 1 | Empty | None | None | None | | ||
| + | | 2 | GFP | [[https://www.micro-shop.zeiss.com/?s=1249886915f0649&l=en&p=us&f=f&a=v&b=f&id=488013-0000-000&o=|FS13]] | 460-480 nm | 505-530 nm | | ||
| + | | 3 | Cy3 | [[https://www.micro-shop.zeiss.com/?s=1249886915f0649&l=en&p=us&f=f&a=v&b=f&id=000000-1114-101&o=|FS43]] | 532-557 nm | 570-640 nm | | ||
| + | | 4 | Cy5.5 | [[https://www.micro-shop.zeiss.com/?s=1249886915f0649&l=en&p=us&f=f&a=v&b=f&id=000000-1031-354&o=|FS32]] | 652-677nm | 700-750 nm | | ||
| + | |||
| + | ==== Objectives ==== | ||
| + | ^ Magnification ^ Model ^ WD (mm) ^ About ^ | ||
| + | | 0.8x | NeoLumar S | 80 | for visual magnification from 6.4x to 80x with eyepieces 10x | | ||
| + | |||
| + | **Upon Request:** | ||
| + | ^ Magnification ^ Model ^ WD (mm) ^ About ^ | ||
| + | | 1.5x | [[https://www.micro-shop.zeiss.com/?s=124984472807e9c&l=en&p=us&f=a&i=10157_400000002300420000&o=&h=25&n=0&sd=435225-9901-000#435225-9901-000|NeoLumar S]] | 30 | for visual magnification from 12x to 150x with eyepieces 10x | | ||
| + | |||
| + | |||
| + | ===== Software Modules ===== | ||
| + | |||
| + | * Multichannel Acquisition | ||
| + | * Z-stack Acquisition | ||
| + | * Extended Focus | ||
| + | |||
| + | ---- | ||
| + | [[resources|Back to the Resources page]] | ||
zeiss_lumar_v12.txt ยท Last modified: 2021/04/09 16:45 by bioimaging
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