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zeiss_lsm_980 [2022/09/02 00:22]
bioimaging [Objectives]
zeiss_lsm_980 [2022/09/02 02:16]
bioimaging
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 </​table>​ </​table>​
 </​html>​ </​html>​
- 
-** page under construction ** 
  
 ===== Microscope overview ===== ===== Microscope overview =====
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 |  40x  |  LD C-Apochromat Corr  |  Water  |  1.10  |  0.62  |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​421867-9970-000/​Objective-LD-C-Apochromat-40x-1.1-W-Corr-M27|421867-9970-000]] ​ | |  40x  |  LD C-Apochromat Corr  |  Water  |  1.10  |  0.62  |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​421867-9970-000/​Objective-LD-C-Apochromat-40x-1.1-W-Corr-M27|421867-9970-000]] ​ |
 |  63x  |  Plan-Apochromat DIC  |  Oil  |  1.40  |  0.19  |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420782-9900-799/​Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-799]] ​ | |  63x  |  Plan-Apochromat DIC  |  Oil  |  1.40  |  0.19  |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420782-9900-799/​Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-799]] ​ |
-==== Filtersets ​(Ocular) ====+==== Filterset ​(Ocular) ====
  
 ^  Position ​ ^  Filterset ​ ^  Reference ​ ^  Excitation ​ ^  Dichroic ​ ^  Emission ​ ^ ^  Position ​ ^  Filterset ​ ^  Reference ​ ^  Excitation ​ ^  Dichroic ​ ^  Emission ​ ^
-|  1  |  ​Green  ​| ​ [[https://​www.micro-shop.zeiss.com/​?​s=7139069fdc79c&​l=en&p=us&​f=f&​a=v&​b=f&​id=489038-9901-000&o=|FS38HE]]  |  ​450-490 nm  |  ​495 nm  |  ​500-550 nm  | +|  1  |  ​QUAD  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/us/​shop/​filterAssistant/​filtersets/​489090-9100-000|  ​FS90HE]]  |  ​370-400 nm  |  ​405 nm  |  ​410-440 nm  | 
-|  ​ ​|  ​Red  ​|  ​[[https://​www.micro-shop.zeiss.com/?​s=163176628fcf844&​l=en&​p=us&​f=f&​a=v&​b=f&​id=000000-1114-101&​o=|FS43]] ​ ​|  ​533-558 nm  |  ​570 nm  |  ​570-640 nm  | +|  ​:::  ​|  ​:::  ​| ​ :::  ​|  ​450-488 nm  |  ​493 nm  |  ​499-529 nm  | 
-|  ​ ​|  ​Blue  ​|  ​[[https://www.micro-shop.zeiss.com/?​s=163176628fcf844&​l=en&​p=us&​f=f&​a=v&​b=f&​id=488049-9901-000&​o=|FS49]] ​ ​|  ​G 365 nm  |  ​395 nm  |  ​420-470 nm  | +|  ​:::  ​|  ​:::  ​| ​ :::  |  540-570 nm  |  575 nm  |  580-605 nm  | 
 + ::: ​ ​|  ​:::  |  :::  |  615-648 ​nm  |  ​653 nm  |  ​659-759 nm  |
 ===== Microscope Turn On Procedure ===== ===== Microscope Turn On Procedure =====
   * Turn on the ''​MAIN SWITCH''​   * Turn on the ''​MAIN SWITCH''​
-{{zeiss_lsm_880_1.jpg?100}} +{{::​zeiss_lsm_980_main_switch.png?100|}} 
-  * Turn on the ''​SYSTEMS/PC'' ​switch+  * Turn on the ''​Laser'' ​key 
 +{{::​zeiss_lsm_980_laser_key.png?​100|}}
   * Turn on the computer   * Turn on the computer
-  * Login in to Windows ​(Bioimaging User)+  * Login in to Windows ​with your Agendo credentials
   * Turn on the ''​COMPONENTS''​ switch   * Turn on the ''​COMPONENTS''​ switch
-  ​* Start the **ZEN Black** software+{{::​zeiss_lsm_980_components.png?​100|}} 
 +  ​* Start the **ZEN** software
  
 ===== Microscope Turn Off Procedure ===== ===== Microscope Turn Off Procedure =====
 If there is another user for this microscope in the __next hour__: If there is another user for this microscope in the __next hour__:
-  * Exit the ZEN software, leave the lasers on+  * Exit the **ZEN** software
   * Log off the computer   * Log off the computer
   * Clean up immersion objectives   * Clean up immersion objectives
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   * Turn off the lasers on **ZEN**   * Turn off the lasers on **ZEN**
   * Clean up immersion objectives   * Clean up immersion objectives
-  * Exit the ZEN software+  * Exit the **ZEN** software
   * Shutdown the computer   * Shutdown the computer
-  * Turn off the two switches ​''​SYSTEMS/PC'' ​and ''​COMPONENTS''​ +  * Turn off the ''​COMPONENTS'' ​switch 
-  * **Wait 5 min. for Ar laser to cool down**+  * Turn off the ''​Laser'' ​key
   * Turn off the ''​MAIN SWITCH''​   * Turn off the ''​MAIN SWITCH''​
  
 ---- ----
 [[resources|Back to the Equipment page]] [[resources|Back to the Equipment page]]
zeiss_lsm_980.txt · Last modified: 2024/04/02 00:22 by bioimaging