zeiss_lsm_980
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zeiss_lsm_980 [2022/09/01 16:55] bioimaging [LASERs] |
zeiss_lsm_980 [2022/09/02 02:12] bioimaging [Microscope Turn Off Procedure] |
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| ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ||
| | 10x | EC Plan-Neofluar | Air | 0.30 | 5.20 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420340-9901-000/Objective-EC-Plan-Neofluar-10x-0.30-M27|420340-9901-000]] | | | 10x | EC Plan-Neofluar | Air | 0.30 | 5.20 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420340-9901-000/Objective-EC-Plan-Neofluar-10x-0.30-M27|420340-9901-000]] | | ||
| - | | 20x | Plan-Apochromat | Air | 0.80 | 0.55 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420650-9901-000/Objective-Plan-Apochromat-20x-0.8-M27|420650-9901-000]] | | + | | 20x | Plan-Apochromat | Air | 0.80 | 0.55 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420650-9903-000/Objective-Plan-Apochromat-20x-0.8-M27|420650-9903-000]] | |
| - | | 40x | C-Apochromat Corr | Water | 1.20 | 0.28 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/421767-9970-000/Objective-C-Apochromat-40x-1.20-W-Corr-M27|421767-9970-000]] | | + | |
| | 40x | LD C-Apochromat Corr | Water | 1.10 | 0.62 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/421867-9970-000/Objective-LD-C-Apochromat-40x-1.1-W-Corr-M27|421867-9970-000]] | | | 40x | LD C-Apochromat Corr | Water | 1.10 | 0.62 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/421867-9970-000/Objective-LD-C-Apochromat-40x-1.1-W-Corr-M27|421867-9970-000]] | | ||
| | 63x | Plan-Apochromat DIC | Oil | 1.40 | 0.19 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420782-9900-799/Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-799]] | | | 63x | Plan-Apochromat DIC | Oil | 1.40 | 0.19 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420782-9900-799/Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-799]] | | ||
| - | + | ==== Filterset (Ocular) ==== | |
| - | + | ||
| - | **Upon request:** | + | |
| - | + | ||
| - | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | + | |
| - | | 25x | LCI Plan-Neofluar Corr DIC | Oil/Glyc/W | 0.80 | 0.21 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420852-9973-000/Objective-LCI-Plan-Neofluar-25x-0.8-Imm-Corr-DIC-M27|420852-9972-000]] | | + | |
| - | | 40x | Plan-Apochromat Corr | Dry | 0.95 | 0.25 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420660-9970-000/Objective-Plan-Apochromat-40x-0.95-Corr-M27|420660-9970-000]] | | + | |
| - | ==== Filtersets (Ocular) ==== | + | |
| ^ Position ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ | ^ Position ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ | ||
| - | | 1 | Green | [[https://www.micro-shop.zeiss.com/?s=7139069fdc79c&l=en&p=us&f=f&a=v&b=f&id=489038-9901-000&o=|FS38HE]] | 450-490 nm | 495 nm | 500-550 nm | | + | | 1 | QUAD | [[https://www.micro-shop.zeiss.com/en/us/shop/filterAssistant/filtersets/489090-9100-000| FS90HE]] | 370-400 nm | 405 nm | 410-440 nm | |
| - | | 2 | Red | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=000000-1114-101&o=|FS43]] | 533-558 nm | 570 nm | 570-640 nm | | + | | ::: | ::: | ::: | 450-488 nm | 493 nm | 499-529 nm | |
| - | | 3 | Blue | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=488049-9901-000&o=|FS49]] | G 365 nm | 395 nm | 420-470 nm | | + | | ::: | ::: | ::: | 540-570 nm | 575 nm | 580-605 nm | |
| + | | ::: | ::: | ::: | 615-648 nm | 653 nm | 659-759 nm | | ||
| ===== Microscope Turn On Procedure ===== | ===== Microscope Turn On Procedure ===== | ||
| * Turn on the ''MAIN SWITCH'' | * Turn on the ''MAIN SWITCH'' | ||
| - | {{zeiss_lsm_880_1.jpg?100}} | + | {{::zeiss_lsm_980_main_switch.png?100|}} |
| - | * Turn on the ''SYSTEMS/PC'' switch | + | * Turn on the ''Laser'' key |
| + | {{::zeiss_lsm_980_laser_key.png?100|}} | ||
| * Turn on the computer | * Turn on the computer | ||
| - | * Login in to Windows (Bioimaging User) | + | * Login in to Windows with your Agendo credentials |
| * Turn on the ''COMPONENTS'' switch | * Turn on the ''COMPONENTS'' switch | ||
| - | * Start the **ZEN Black** software | + | {{::zeiss_lsm_980_components.png?100|}} |
| + | * Start the **ZEN** software | ||
| ===== Microscope Turn Off Procedure ===== | ===== Microscope Turn Off Procedure ===== | ||
| If there is another user for this microscope in the __next hour__: | If there is another user for this microscope in the __next hour__: | ||
| - | * Exit the ZEN software, leave the lasers on | + | * Exit the ZEN software |
| * Log off the computer | * Log off the computer | ||
| * Clean up immersion objectives | * Clean up immersion objectives | ||
| Line 96: | Line 90: | ||
| * Exit the ZEN software | * Exit the ZEN software | ||
| * Shutdown the computer | * Shutdown the computer | ||
| - | * Turn off the two switches ''SYSTEMS/PC'' and ''COMPONENTS'' | + | * Turn off the ''COMPONENTS'' switch |
| - | * **Wait 5 min. for Ar laser to cool down** | + | * Switch off the ''Laser'' key |
| * Turn off the ''MAIN SWITCH'' | * Turn off the ''MAIN SWITCH'' | ||
| ---- | ---- | ||
| [[resources|Back to the Equipment page]] | [[resources|Back to the Equipment page]] | ||
zeiss_lsm_980.txt · Last modified: 2024/04/02 00:22 by bioimaging
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