zeiss_cell_observer_sd
Differences
This shows you the differences between two versions of the page.
| Both sides previous revision Previous revision Next revision | Previous revision Next revision Both sides next revision | ||
|
zeiss_cell_observer_sd [2022/09/02 02:13] bioimaging [Microscope Turn On Procedure] |
zeiss_cell_observer_sd [2023/03/27 13:02] bioimaging |
||
|---|---|---|---|
| Line 14: | Line 14: | ||
| <b>SN</b>: 3851001637 <br> | <b>SN</b>: 3851001637 <br> | ||
|   <br> |   <br> | ||
| + | Data will be deleted after: <b>1 month</b> | ||
|   <br> |   <br> | ||
|   <br> |   <br> | ||
| Line 49: | Line 50: | ||
| ===== System components ===== | ===== System components ===== | ||
| - | |||
| - | ==== LASERs ==== | ||
| - | ^ Laser Unit ^ Wavelength ^ Maximum Power ^ | ||
| - | | Solid State Laser 405 | 405 nm | 50 mW | | ||
| - | | Solid State Laser 488 | 488 nm | 100 mW | | ||
| - | | Solid State Laser 561 | 561 nm | 75 mW | | ||
| - | | Solid State Laser 638 | 638 nm | 75 mW | | ||
| ==== Objectives ==== | ==== Objectives ==== | ||
| Line 71: | Line 65: | ||
| | 10x | EC Plan-Neofluar | Air | 0.30 | 5.20 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420340-9901-000/Objective-EC-Plan-Neofluar-10x-0.30-M27|420340-9901-000]] | | | 10x | EC Plan-Neofluar | Air | 0.30 | 5.20 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420340-9901-000/Objective-EC-Plan-Neofluar-10x-0.30-M27|420340-9901-000]] | | ||
| | 25x | LCI Plan-Neofluar Corr DIC | Oil/Glyc/W | 0.80 | 0.21 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420852-9972-000/Objective-LCI-Plan-Neofluar-25x-0.8-Imm-Corr-DIC-M27|420852-9972-000]] | | | 25x | LCI Plan-Neofluar Corr DIC | Oil/Glyc/W | 0.80 | 0.21 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420852-9972-000/Objective-LCI-Plan-Neofluar-25x-0.8-Imm-Corr-DIC-M27|420852-9972-000]] | | ||
| + | |||
| + | ==== LASERs (Confocal) ==== | ||
| + | ^ Laser Unit ^ Wavelength ^ Maximum Power ^ | ||
| + | | Solid State Laser 405 | 405 nm | 50 mW | | ||
| + | | Solid State Laser 488 | 488 nm | 100 mW | | ||
| + | | Solid State Laser 561 | 561 nm | 75 mW | | ||
| + | | Solid State Laser 638 | 638 nm | 75 mW | | ||
| + | |||
| + | ==== Emission Filters (Confocal) ==== | ||
| + | |||
| + | ^ Position ^ Transmission ^ | ||
| + | | 1: BP 450/50 | 425-475 nm | | ||
| + | | 2: TBP 526 601 688 | 510-542 nm | | ||
| + | | ::: | 586-616 nm | | ||
| + | | ::: | 663-713 nm | | ||
| + | | 3: none | | | ||
| + | | 4: FE01-520/35 | 502-537 nm | | ||
| + | | 5: BP 600 50 | 575-625 nm | | ||
| + | | 6: BP 690/50 | 665-715 nm | | ||
| + | |||
| + | ==== LEDs (Widefield) ==== | ||
| + | ^ Line ^ Wavelength ^ | ||
| + | | UV | 365 nm | | ||
| + | | B | 470 nm | | ||
| + | | G | 565 nm | | ||
| + | | R | 625 nm | | ||
| ==== Filtersets (Widefield) ==== | ==== Filtersets (Widefield) ==== | ||
| Line 85: | Line 105: | ||
| | 6 | Empty ||||| | | 6 | Empty ||||| | ||
| - | ==== Emission Filters (Confocal) ==== | ||
| - | |||
| - | ^ Position ^ Transmission ^ | ||
| - | | 1: BP 450/50 | 425-475 nm | | ||
| - | | 2: TBP 526 601 688 | 510-542 nm | | ||
| - | | ::: | 586-616 nm | | ||
| - | | ::: | 663-713 nm | | ||
| - | | 3: none | | | ||
| - | | 4: FE01-520/35 | 502-537 nm | | ||
| - | | 5: BP 600 50 | 575-625 nm | | ||
| - | | 6: BP 690/50 | 665-715 nm | | ||
| ==== Cameras ==== | ==== Cameras ==== | ||
| - | ^ Model ^ Type ^ Frame Size ^ Pixel Size (µm) ^ Quantum Efficiency ^ | + | ^ Model ^ Type ^ Frame Size ^ Pixel Size (µm) ^ Quantum Efficiency ^ Mode ^ |
| - | | [[https://www.photometrics.com/wp-content/uploads/2019/10/Evolve512-Datasheet.pdf|Photometrics Evolve 512]] | EMCCD | 512 x 512 | 16 x 16 | > 90% | | + | | [[https://www.photometrics.com/wp-content/uploads/2019/10/Evolve512-Datasheet.pdf|Photometrics Evolve 512]] | EMCCD | 512 x 512 | 16 x 16 | > 90% | Confocal | |
| - | | [[https://www.hamamatsu.com/resources/pdf/sys/SCAS0081E_C11440-22CU.pdf|Hamamatsu ORCA-flash4.0 V2]] | sCMOS | 2048 x 2048 | 6.5 x 6.5 | 82% | | + | | [[https://www.hamamatsu.com/resources/pdf/sys/SCAS0081E_C11440-22CU.pdf|Hamamatsu ORCA-flash4.0 V2]] | sCMOS | 2048 x 2048 | 6.5 x 6.5 | 82% | Widefield | |
| ===== Microscope Turn On Procedure ===== | ===== Microscope Turn On Procedure ===== | ||
zeiss_cell_observer_sd.txt · Last modified: 2024/04/01 23:53 by bioimaging
Page Tools
