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zeiss_cell_observer_sd [2022/09/01 11:44] bioimaging |
zeiss_cell_observer_sd [2022/09/02 12:56] bioimaging |
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<td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
<b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_sd_map_new.png">Room P2-A-22</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47217) <br> | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_sd_map_new.png">Room P2-A-22</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47217) <br> | ||
- | <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">Carl Zeiss Microimaging</a><br> | + | <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">ZEISS Microscopy</a><br> |
<b>Model</b>: Cell Observer SD<br> | <b>Model</b>: Cell Observer SD<br> | ||
<b>Nickname</b>: "Cell Observer SD"<br> | <b>Nickname</b>: "Cell Observer SD"<br> | ||
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===== System components ===== | ===== System components ===== | ||
- | |||
- | ==== LASERs ==== | ||
- | ^ Laser Unit ^ Wavelength ^ Maximum Power ^ | ||
- | | Solid State Laser 405 | 405 nm | 50 mW | | ||
- | | Solid State Laser 488 | 488 nm | 100 mW | | ||
- | | Solid State Laser 561 | 561 nm | 75 mW | | ||
- | | Solid State Laser 638 | 638 nm | 75 mW | | ||
==== Objectives ==== | ==== Objectives ==== | ||
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| 10x | EC Plan-Neofluar | Air | 0.30 | 5.20 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420340-9901-000/Objective-EC-Plan-Neofluar-10x-0.30-M27|420340-9901-000]] | | | 10x | EC Plan-Neofluar | Air | 0.30 | 5.20 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420340-9901-000/Objective-EC-Plan-Neofluar-10x-0.30-M27|420340-9901-000]] | | ||
| 25x | LCI Plan-Neofluar Corr DIC | Oil/Glyc/W | 0.80 | 0.21 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420852-9972-000/Objective-LCI-Plan-Neofluar-25x-0.8-Imm-Corr-DIC-M27|420852-9972-000]] | | | 25x | LCI Plan-Neofluar Corr DIC | Oil/Glyc/W | 0.80 | 0.21 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420852-9972-000/Objective-LCI-Plan-Neofluar-25x-0.8-Imm-Corr-DIC-M27|420852-9972-000]] | | ||
+ | |||
+ | ==== LASERs (Confocal) ==== | ||
+ | ^ Laser Unit ^ Wavelength ^ Maximum Power ^ | ||
+ | | Solid State Laser 405 | 405 nm | 50 mW | | ||
+ | | Solid State Laser 488 | 488 nm | 100 mW | | ||
+ | | Solid State Laser 561 | 561 nm | 75 mW | | ||
+ | | Solid State Laser 638 | 638 nm | 75 mW | | ||
+ | |||
+ | ==== Emission Filters (Confocal) ==== | ||
+ | |||
+ | ^ Position ^ Transmission ^ | ||
+ | | 1: BP 450/50 | 425-475 nm | | ||
+ | | 2: TBP 526 601 688 | 510-542 nm | | ||
+ | | ::: | 586-616 nm | | ||
+ | | ::: | 663-713 nm | | ||
+ | | 3: none | | | ||
+ | | 4: FE01-520/35 | 502-537 nm | | ||
+ | | 5: BP 600 50 | 575-625 nm | | ||
+ | | 6: BP 690/50 | 665-715 nm | | ||
+ | |||
+ | ==== LEDs (Widefield) ==== | ||
+ | ^ Line ^ Wavelength ^ | ||
+ | | UV | 365 nm | | ||
+ | | B | 470 nm | | ||
+ | | G | 565 nm | | ||
+ | | R | 625 nm | | ||
==== Filtersets (Widefield) ==== | ==== Filtersets (Widefield) ==== | ||
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| 6 | Empty ||||| | | 6 | Empty ||||| | ||
- | ==== Emission Filters (Confocal) ==== | ||
- | |||
- | ^ Position ^ Transmission ^ | ||
- | | 1: BP 450/50 | 425-475 nm | | ||
- | | 2: TBP 526 601 688 | 510-542 nm | | ||
- | | ::: | 586-616 nm | | ||
- | | ::: | 663-713 nm | | ||
- | | 3: none | | | ||
- | | 4: FE01-520/35 | 502-537 nm | | ||
- | | 5: BP 600 50 | 575-625 nm | | ||
- | | 6: BP 690/50 | 665-715 nm | | ||
==== Cameras ==== | ==== Cameras ==== | ||
- | ^ Model ^ Type ^ Frame Size ^ Pixel Size (µm) ^ Quantum Efficiency ^ | + | ^ Model ^ Type ^ Frame Size ^ Pixel Size (µm) ^ Quantum Efficiency ^ Mode ^ |
- | | [[https://www.photometrics.com/wp-content/uploads/2019/10/Evolve512-Datasheet.pdf|Photometrics Evolve 512]] | EMCCD | 512 x 512 | 16 x 16 | > 90% | | + | | [[https://www.photometrics.com/wp-content/uploads/2019/10/Evolve512-Datasheet.pdf|Photometrics Evolve 512]] | EMCCD | 512 x 512 | 16 x 16 | > 90% | Confocal | |
- | | [[https://www.hamamatsu.com/resources/pdf/sys/SCAS0081E_C11440-22CU.pdf|Hamamatsu ORCA-flash4.0 V2]] | sCMOS | 2048 x 2048 | 6.5 x 6.5 | 82% | | + | | [[https://www.hamamatsu.com/resources/pdf/sys/SCAS0081E_C11440-22CU.pdf|Hamamatsu ORCA-flash4.0 V2]] | sCMOS | 2048 x 2048 | 6.5 x 6.5 | 82% | Widefield | |
===== Microscope Turn On Procedure ===== | ===== Microscope Turn On Procedure ===== | ||
* Turn on the HXP 200 fluorescent light source (if used) | * Turn on the HXP 200 fluorescent light source (if used) | ||
* Turn on the computer (underneath the microscope table) | * Turn on the computer (underneath the microscope table) | ||
- | * Login in to Windows (Bioimaging User) | + | * Login in to Windows with your Agendo credentials |
* Turn on the black socket outlet **number 1** (back left corner of microscope table) | * Turn on the black socket outlet **number 1** (back left corner of microscope table) | ||
{{::sd_power.png?200|}} | {{::sd_power.png?200|}} |