zeiss_cell_observer_sd
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zeiss_cell_observer_sd [2022/08/30 12:26] bioimaging [LASERs] |
zeiss_cell_observer_sd [2023/03/27 13:02] bioimaging |
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| - | <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_sd_wiki_new.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_sd_wiki_new.png" width=300></a> | + | <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_sd_wiki_2022.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_sd_wiki_2022.png" width=300></a> |
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_sd_map_new.png">Room P2-A-22</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47217) <br> | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_sd_map_new.png">Room P2-A-22</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47217) <br> | ||
| - | <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">Carl Zeiss Microimaging</a><br> | + | <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">ZEISS Microscopy</a><br> |
| <b>Model</b>: Cell Observer SD<br> | <b>Model</b>: Cell Observer SD<br> | ||
| <b>Nickname</b>: "Cell Observer SD"<br> | <b>Nickname</b>: "Cell Observer SD"<br> | ||
| - | <b>Software</b>: ZEN 2.6 (blue edition)<br> | + | <b>Software</b>: ZEN 3.2 (blue edition)<br> |
| <b>Year</b>: 2015 <br> | <b>Year</b>: 2015 <br> | ||
| <b>SN</b>: 3851001637 <br> | <b>SN</b>: 3851001637 <br> | ||
| + |   <br> | ||
| + | Data will be deleted after: <b>1 month</b> | ||
|   <br> |   <br> | ||
|   <br> |   <br> | ||
|   <br> |   <br> | ||
| - | → <a href="https://my.agendo.science/calendar/?id=MjVjYWxlbmRhcnM="><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> Zeiss Cell Observer SD Booking</a> | ||
| - | <br> | ||
| → <a href="/facility/bioimaging/doku.php?id=zeiss_cell_observer_sd_quality"><img src="/facility/bioimaging/lib/exe/fetch.php?media=list-icon.png" width=18> Zeiss Cell Observer SD Quality Control</a> | → <a href="/facility/bioimaging/doku.php?id=zeiss_cell_observer_sd_quality"><img src="/facility/bioimaging/lib/exe/fetch.php?media=list-icon.png" width=18> Zeiss Cell Observer SD Quality Control</a> | ||
| <br> | <br> | ||
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| ===== System components ===== | ===== System components ===== | ||
| - | |||
| - | ==== LASERs ==== | ||
| - | ^ Laser Unit ^ Wavelength ^ Maximum Power ^ | ||
| - | | Solid State Laser 405 | 405 nm | 50 mW | | ||
| - | | Solid State Laser 488 | 488 nm | 100 mW | | ||
| - | | Solid State Laser 561 | 561 nm | 75 mW | | ||
| - | | Solid State Laser 638 | 638 nm | 75 mW | | ||
| ==== Objectives ==== | ==== Objectives ==== | ||
| Line 72: | Line 65: | ||
| | 10x | EC Plan-Neofluar | Air | 0.30 | 5.20 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420340-9901-000/Objective-EC-Plan-Neofluar-10x-0.30-M27|420340-9901-000]] | | | 10x | EC Plan-Neofluar | Air | 0.30 | 5.20 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420340-9901-000/Objective-EC-Plan-Neofluar-10x-0.30-M27|420340-9901-000]] | | ||
| | 25x | LCI Plan-Neofluar Corr DIC | Oil/Glyc/W | 0.80 | 0.21 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420852-9972-000/Objective-LCI-Plan-Neofluar-25x-0.8-Imm-Corr-DIC-M27|420852-9972-000]] | | | 25x | LCI Plan-Neofluar Corr DIC | Oil/Glyc/W | 0.80 | 0.21 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420852-9972-000/Objective-LCI-Plan-Neofluar-25x-0.8-Imm-Corr-DIC-M27|420852-9972-000]] | | ||
| + | |||
| + | ==== LASERs (Confocal) ==== | ||
| + | ^ Laser Unit ^ Wavelength ^ Maximum Power ^ | ||
| + | | Solid State Laser 405 | 405 nm | 50 mW | | ||
| + | | Solid State Laser 488 | 488 nm | 100 mW | | ||
| + | | Solid State Laser 561 | 561 nm | 75 mW | | ||
| + | | Solid State Laser 638 | 638 nm | 75 mW | | ||
| + | |||
| + | ==== Emission Filters (Confocal) ==== | ||
| + | |||
| + | ^ Position ^ Transmission ^ | ||
| + | | 1: BP 450/50 | 425-475 nm | | ||
| + | | 2: TBP 526 601 688 | 510-542 nm | | ||
| + | | ::: | 586-616 nm | | ||
| + | | ::: | 663-713 nm | | ||
| + | | 3: none | | | ||
| + | | 4: FE01-520/35 | 502-537 nm | | ||
| + | | 5: BP 600 50 | 575-625 nm | | ||
| + | | 6: BP 690/50 | 665-715 nm | | ||
| + | |||
| + | ==== LEDs (Widefield) ==== | ||
| + | ^ Line ^ Wavelength ^ | ||
| + | | UV | 365 nm | | ||
| + | | B | 470 nm | | ||
| + | | G | 565 nm | | ||
| + | | R | 625 nm | | ||
| ==== Filtersets (Widefield) ==== | ==== Filtersets (Widefield) ==== | ||
| Line 86: | Line 105: | ||
| | 6 | Empty ||||| | | 6 | Empty ||||| | ||
| - | ==== Emission Filters (Confocal) ==== | ||
| - | |||
| - | ^ Position ^ Transmission ^ | ||
| - | | 1: BP 450/50 | 425-475 nm | | ||
| - | | 2: TBP 526 601 688 | 510-542 nm | | ||
| - | | ::: | 586-616 nm | | ||
| - | | ::: | 663-713 nm | | ||
| - | | 3: none | | | ||
| - | | 4: FE01-520/35 | 502-537 nm | | ||
| - | | 5: BP 600 50 | 575-625 nm | | ||
| - | | 6: BP 690/50 | 665-715 nm | | ||
| ==== Cameras ==== | ==== Cameras ==== | ||
| - | ^ Model ^ Type ^ Frame Size ^ Pixel Size (µm) ^ Quantum Efficiency ^ | + | ^ Model ^ Type ^ Frame Size ^ Pixel Size (µm) ^ Quantum Efficiency ^ Mode ^ |
| - | | [[https://www.photometrics.com/wp-content/uploads/2019/10/Evolve512-Datasheet.pdf|Photometrics Evolve 512]] | EMCCD | 512 x 512 | 16 x 16 | > 90% | | + | | [[https://www.photometrics.com/wp-content/uploads/2019/10/Evolve512-Datasheet.pdf|Photometrics Evolve 512]] | EMCCD | 512 x 512 | 16 x 16 | > 90% | Confocal | |
| - | | [[https://www.hamamatsu.com/resources/pdf/sys/SCAS0081E_C11440-22CU.pdf|Hamamatsu ORCA-flash4.0 V2]] | sCMOS | 2048 x 2048 | 6.5 x 6.5 | 82% | | + | | [[https://www.hamamatsu.com/resources/pdf/sys/SCAS0081E_C11440-22CU.pdf|Hamamatsu ORCA-flash4.0 V2]] | sCMOS | 2048 x 2048 | 6.5 x 6.5 | 82% | Widefield | |
| ===== Microscope Turn On Procedure ===== | ===== Microscope Turn On Procedure ===== | ||
| * Turn on the HXP 200 fluorescent light source (if used) | * Turn on the HXP 200 fluorescent light source (if used) | ||
| * Turn on the computer (underneath the microscope table) | * Turn on the computer (underneath the microscope table) | ||
| - | * Login in to Windows (Bioimaging User) | + | * Login in to Windows with your Agendo credentials |
| * Turn on the black socket outlet **number 1** (back left corner of microscope table) | * Turn on the black socket outlet **number 1** (back left corner of microscope table) | ||
| {{::sd_power.png?200|}} | {{::sd_power.png?200|}} | ||
zeiss_cell_observer_sd.txt · Last modified: 2024/04/01 23:53 by bioimaging
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