zeiss_cell_observer_sd
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| Both sides previous revision Previous revision Next revision | Previous revision Next revision Both sides next revision | ||
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zeiss_cell_observer_sd [2020/01/01 17:23] bioimaging [Filtersets (Widefield)] |
zeiss_cell_observer_sd [2020/01/17 13:07] bioimaging [Emission Filtersets (Confocal)] |
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| * **EMCCD Camera**: [[https://www.photometrics.com/wp-content/uploads/2019/10/Evolve512-Datasheet.pdf|Evolve 512 EMCCD]] | * **EMCCD Camera**: [[https://www.photometrics.com/wp-content/uploads/2019/10/Evolve512-Datasheet.pdf|Evolve 512 EMCCD]] | ||
| * **sCMOS Camera**: [[https://www.hamamatsu.com/resources/pdf/sys/SCAS0081E_C11440-22CU.pdf|Hamamatsu ORCA-flash4.0 V2]] | * **sCMOS Camera**: [[https://www.hamamatsu.com/resources/pdf/sys/SCAS0081E_C11440-22CU.pdf|Hamamatsu ORCA-flash4.0 V2]] | ||
| + | * [[https://www.zeiss.com/microscopy/int/products/light-microscopes/axio-observer-for-biology/definite-focus.html|Definite Focus.2]] | ||
| {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | ||
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| | 63x | Plan-Apochromat Ph3 | Oil | 1.40 | 0.19 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420781-9910-000/Objective-Plan-Apochromat-63-1.4-Oil-Ph3-M27|420781-9910-000]] | | | 63x | Plan-Apochromat Ph3 | Oil | 1.40 | 0.19 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420781-9910-000/Objective-Plan-Apochromat-63-1.4-Oil-Ph3-M27|420781-9910-000]] | | ||
| | 100x | α Plan-Apochromat DIC | Oil | 1.46 | 0.11 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420792-9800-000/Objective-alpha-Plan-Apochromat-100x-1.46-Oil-DIC-M27|420792-9800-000]] | | | 100x | α Plan-Apochromat DIC | Oil | 1.46 | 0.11 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420792-9800-000/Objective-alpha-Plan-Apochromat-100x-1.46-Oil-DIC-M27|420792-9800-000]] | | ||
| - | |||
| - | <html> | ||
| - | <br> | ||
| - | <br> | ||
| - | <br> | ||
| - | <br> | ||
| - | </html> | ||
| ==== Filtersets (Widefield) ==== | ==== Filtersets (Widefield) ==== | ||
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| | ::: | ::: | ::: | 620-642 nm | 658-704 nm | | | ::: | ::: | ::: | 620-642 nm | 658-704 nm | | ||
| | 6 | none | | | | | | 6 | none | | | | | ||
| - | + | ==== Emission Filters (Confocal) ==== | |
| - | <html> | + | |
| - | <br> | + | |
| - | </html> | + | |
| - | + | ||
| - | ==== Emission Filtersets (Confocal) ==== | + | |
| - | + | ||
| - | {{:zeiss_cell_observer_sd_filters_confocal.jpg |}} | + | |
| ^ Position ^ Transmission ^ | ^ Position ^ Transmission ^ | ||
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| | 5: BP 600 50 | 575-625 nm | | | 5: BP 600 50 | 575-625 nm | | ||
| | 6: BP 690/50 | 665-715 nm | | | 6: BP 690/50 | 665-715 nm | | ||
| + | |||
| + | ==== Cameras ==== | ||
| + | ^ Model ^ Type ^ Frame Size ^ Pixel Size (µm) ^ Quantum Efficiency ^ | ||
| + | | [[https://www.photometrics.com/wp-content/uploads/2019/10/Evolve512-Datasheet.pdf|Photometrics Evolve 512]] | EMCCD | 512 x 512 | 16 x 16 | > 90% | | ||
| + | | [[https://www.hamamatsu.com/resources/pdf/sys/SCAS0081E_C11440-22CU.pdf|Hamamatsu ORCA-flash4.0 V2]] | sCMOS | 2048 x 2048 | 6.5 x 6.5 | 82% | | ||
| ---- | ---- | ||
| [[resources|Back to the Resources page]] | [[resources|Back to the Resources page]] | ||
zeiss_cell_observer_sd.txt · Last modified: 2024/04/01 23:53 by bioimaging
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