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zeiss_cell_observer_sd [2020/01/01 17:23]
bioimaging [Objectives]
zeiss_cell_observer_sd [2020/01/01 17:27]
bioimaging [Emission Filtersets (Confocal)]
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 |  63x  |  Plan-Apochromat Ph3  |  Oil  |  1.40  |  0.19  |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420781-9910-000/​Objective-Plan-Apochromat-63-1.4-Oil-Ph3-M27|420781-9910-000]] ​ | |  63x  |  Plan-Apochromat Ph3  |  Oil  |  1.40  |  0.19  |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420781-9910-000/​Objective-Plan-Apochromat-63-1.4-Oil-Ph3-M27|420781-9910-000]] ​ |
 |  100x  |  α Plan-Apochromat DIC  |  Oil  |  1.46  |  0.11  |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420792-9800-000/​Objective-alpha-Plan-Apochromat-100x-1.46-Oil-DIC-M27|420792-9800-000]] ​ | |  100x  |  α Plan-Apochromat DIC  |  Oil  |  1.46  |  0.11  |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420792-9800-000/​Objective-alpha-Plan-Apochromat-100x-1.46-Oil-DIC-M27|420792-9800-000]] ​ |
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 ==== Filtersets (Widefield) ==== ==== Filtersets (Widefield) ====
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-{{:​zeiss_cell_observer_sd_filters_widefield.jpg ​ |}} 
  
 ^  Position ​ ^  Filterset ​ ^  Reference ​ ^  Excitation ​ ^  Emission ​ ^ ^  Position ​ ^  Filterset ​ ^  Reference ​ ^  Excitation ​ ^  Emission ​ ^
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 |  :::  |  :::  |  :::  |  620-642 nm  |  658-704 nm  | |  :::  |  :::  |  :::  |  620-642 nm  |  658-704 nm  |
 |  6  |  none  |  |  |  | |  6  |  none  |  |  |  |
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 ==== Emission Filtersets (Confocal) ==== ==== Emission Filtersets (Confocal) ====
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-{{:​zeiss_cell_observer_sd_filters_confocal.jpg ​ |}} 
  
 ^  Position ​ ^  Transmission ​ ^ ^  Position ​ ^  Transmission ​ ^
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 |  5: BP 600 50  |  575-625 nm  | |  5: BP 600 50  |  575-625 nm  |
 |  6: BP 690/​50 ​ |  665-715 nm  | |  6: BP 690/​50 ​ |  665-715 nm  |
 +
 +==== Cameras ====
 +^  Model  ^  Type  ^  Frame Size  ^  Pixel Size (µm)  ^  Quantum Efficiency ​ ^
 +|  [[https://​www.photometrics.com/​wp-content/​uploads/​2019/​10/​Evolve512-Datasheet.pdf|Photometrics Evolve 512]]  |  EMCCD  |  512 x 512  |  16 x 16  |  > 90%  |
 +|  [[https://​www.hamamatsu.com/​resources/​pdf/​sys/​SCAS0081E_C11440-22CU.pdf|Hamamatsu ORCA-flash4.0 V2]]  |  sCMOS  |  2048 x 2048  |  6.5 x 6.5  |  82%  |
  
 ---- ----
  
 [[resources|Back to the Resources page]] [[resources|Back to the Resources page]]
zeiss_cell_observer_sd.txt · Last modified: 2024/04/01 23:53 by bioimaging