zeiss_cell_observer_sd
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zeiss_cell_observer_sd [2016/07/01 13:59] bioimaging [Emission Filtersets (Confocal)] |
zeiss_cell_observer_sd [2017/03/29 11:46] bioimaging |
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| - | <a href="/facility/bioimaging/lib/exe/fetch.php?media=3i_marianas_sdc_wiki.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=3i_marianas_sdc_wiki.jpg" width=300></a> | + | <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_sd_wiki.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_sd_wiki.jpg" width=300></a> |
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| <b>Location</b>: Room P2-A-22 (<img src="/facility/bioimaging/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47217) <br> | <b>Location</b>: Room P2-A-22 (<img src="/facility/bioimaging/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47217) <br> | ||
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| ===== System overview ===== | ===== System overview ===== | ||
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| {{ :spinning_disk.jpg?0x300|}} The Zeiss Cell Observer SD //spinning disk confocal microscope// is a fast imaging system which provides a trade-off between confocality, resolution and speed. It is an inverted microscope ideal for live cell applications which require fast acquisition speeds rather than high resolution images. The scanning unit achieves confocality by directing light through a spinning disk with many small pinholes. Images are then acquired with a sensitive EMCCD which allows for very small exposure times but is limited in resolution to 512x512 pixels. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions. The system is also equipped with a large size incubator for temperature control, a small stage incubator for CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. You can also use the system as a widefield microscope, using a sCMOS camera for acquisition. | {{ :spinning_disk.jpg?0x300|}} The Zeiss Cell Observer SD //spinning disk confocal microscope// is a fast imaging system which provides a trade-off between confocality, resolution and speed. It is an inverted microscope ideal for live cell applications which require fast acquisition speeds rather than high resolution images. The scanning unit achieves confocality by directing light through a spinning disk with many small pinholes. Images are then acquired with a sensitive EMCCD which allows for very small exposure times but is limited in resolution to 512x512 pixels. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions. The system is also equipped with a large size incubator for temperature control, a small stage incubator for CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. You can also use the system as a widefield microscope, using a sCMOS camera for acquisition. | ||
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| ==== Objectives ==== | ==== Objectives ==== | ||
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| + | {{:zeiss_cell_observer_sd_objectives.jpg |}} | ||
| ^ Magnification ^ Model ^ Type ^ NA ^ WD (mm) ^ | ^ Magnification ^ Model ^ Type ^ NA ^ WD (mm) ^ | ||
| | 20x | [[https://www.micro-shop.zeiss.com/?s=42373720ae8af6&l=en&p=us&f=o&a=v&m=s&id=420651-9911-000|Plan-Apochromat]] | Dry | 0.8 | 0.55 | | | 20x | [[https://www.micro-shop.zeiss.com/?s=42373720ae8af6&l=en&p=us&f=o&a=v&m=s&id=420651-9911-000|Plan-Apochromat]] | Dry | 0.8 | 0.55 | | ||
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| | 40x | [[https://www.micro-shop.zeiss.com/?s=42373720ae8af6&l=en&p=us&f=o&a=v&m=s&id=420762-9900-000|Plan-Apochromat]] | Oil | 1.4 | 0.13 | | | 40x | [[https://www.micro-shop.zeiss.com/?s=42373720ae8af6&l=en&p=us&f=o&a=v&m=s&id=420762-9900-000|Plan-Apochromat]] | Oil | 1.4 | 0.13 | | ||
| | 63x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=420782-9900-000|Plan-Apochromat]] | Oil | 1.40 | 0.19 | | | 63x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=420782-9900-000|Plan-Apochromat]] | Oil | 1.40 | 0.19 | | ||
| + | | 63x | [[https://www.micro-shop.zeiss.com/?s=4238899732b41f&l=en&p=us&f=o&a=v&m=s&id=420781-9910-000|Plan-Apochromat Ph3]] | Oil | 1.40 | 0.19 | | ||
| | 100x | [[https://www.micro-shop.zeiss.com/?s=7138707e1a1de&l=en&p=uk&f=o&a=v&m=s&id=420792-9800-000|alpha Plan-Apochromat]] | Oil | 1.46 | 0.11 | | | 100x | [[https://www.micro-shop.zeiss.com/?s=7138707e1a1de&l=en&p=uk&f=o&a=v&m=s&id=420792-9800-000|alpha Plan-Apochromat]] | Oil | 1.46 | 0.11 | | ||
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| + | <html> | ||
| + | <br> | ||
| + | <br> | ||
| + | <br> | ||
| + | <br> | ||
| + | </html> | ||
| ==== Filtersets (Widefield) ==== | ==== Filtersets (Widefield) ==== | ||
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| + | {{:zeiss_cell_observer_sd_filters_widefield.jpg |}} | ||
| ^ Position ^ Filterset ^ Reference ^ Excitation ^ Emission ^ | ^ Position ^ Filterset ^ Reference ^ Excitation ^ Emission ^ | ||
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| | ::: | ::: | ::: | 620-642 nm | 658-704 nm | | | ::: | ::: | ::: | 620-642 nm | 658-704 nm | | ||
| | 6 | none | | | | | | 6 | none | | | | | ||
| + | |||
| + | <html> | ||
| + | <br> | ||
| + | </html> | ||
| ==== Emission Filtersets (Confocal) ==== | ==== Emission Filtersets (Confocal) ==== | ||
| + | |||
| + | {{:zeiss_cell_observer_sd_filters_confocal.jpg |}} | ||
| + | |||
| ^ Position ^ Transmission ^ | ^ Position ^ Transmission ^ | ||
| | 1: BP 450/50 | 425-475 nm | | | 1: BP 450/50 | 425-475 nm | | ||
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| | 5: BP 600 50 | 575-625 nm | | | 5: BP 600 50 | 575-625 nm | | ||
| | 6: BP 690/50 | 665-715 nm | | | 6: BP 690/50 | 665-715 nm | | ||
| - | |||
| - | ===== Microscope Turn On Procedure ===== | ||
| - | * Check that the main power supply switch is on (should be on by default) | ||
| - | {{marianas_power1.jpg?|}} | ||
| - | * Turn on the fluorescence lamp (if needed) | ||
| - | {{marianas_power5.jpg?|}} | ||
| - | * Turn on the secondary power supply switch | ||
| - | {{marianas_power2.jpg?|}} | ||
| - | * Turn on the power switch distributor (green) and the component power switches (red) **one at a time** | ||
| - | {{marianas_power3.jpg?|}} | ||
| - | * Turn on the Yokogawa spinning disk scanner (using the key) | ||
| - | {{marianas_power4.jpg?|}} | ||
| - | * Turn on incubation and CO2 on the touchscreen (if needed) | ||
| - | {{marianas_power6.jpg?|}} | ||
| - | * Turn on the lasers you need to use (using the key and the power switches) | ||
| - | {{marianas_power7.jpg?|}} | ||
| - | * Turn on the computer | ||
| - | * Wait 2 min to allow for system components detection | ||
| - | * Start the **SlideBook** software | ||
| - | |||
| - | **Warning**: If you need to change the stage adaptor, please contact the <html><a href="/facility/bioimaging/doku.php?id=about">Bioimaging Facility</a> (<b>imm-bioimaging@medicina.ulisboa.pt</b> | <img src="/facility/bioimaging/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47316</html>) | ||
| - | |||
| - | ===== Microscope Turn Off Procedure ===== | ||
| - | * Remove your sample from the stage/piezo holder | ||
| - | * Exit the **SlideBook** software | ||
| - | * Switch off the computer | ||
| - | * Turn off all system components (follow the Turn On Procedure in reverse) | ||
| ---- | ---- | ||
| [[resources|Back to the Resources page]] | [[resources|Back to the Resources page]] | ||
zeiss_cell_observer_sd.txt ยท Last modified: 2024/04/01 23:53 by bioimaging
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