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zeiss_cell_observer_sd [2016/07/01 13:59] bioimaging [Emission Filtersets (Confocal)] |
zeiss_cell_observer_sd [2016/07/01 15:20] bioimaging |
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- | <a href="/facility/bioimaging/lib/exe/fetch.php?media=3i_marianas_sdc_wiki.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=3i_marianas_sdc_wiki.jpg" width=300></a> | + | <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_sd_wiki.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_sd_wiki.jpg" width=300></a> |
<td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
<b>Location</b>: Room P2-A-22 (<img src="/facility/bioimaging/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47217) <br> | <b>Location</b>: Room P2-A-22 (<img src="/facility/bioimaging/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47217) <br> | ||
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| ::: | 586-616 nm | | | ::: | 586-616 nm | | ||
| ::: | 663-713 nm | | | ::: | 663-713 nm | | ||
- | | 3: none | | | + | | 3: none | | |
| 4: FE01-520/35 | 502-537 nm | | | 4: FE01-520/35 | 502-537 nm | | ||
| 5: BP 600 50 | 575-625 nm | | | 5: BP 600 50 | 575-625 nm | | ||
| 6: BP 690/50 | 665-715 nm | | | 6: BP 690/50 | 665-715 nm | | ||
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- | ===== Microscope Turn On Procedure ===== | ||
- | * Check that the main power supply switch is on (should be on by default) | ||
- | {{marianas_power1.jpg?|}} | ||
- | * Turn on the fluorescence lamp (if needed) | ||
- | {{marianas_power5.jpg?|}} | ||
- | * Turn on the secondary power supply switch | ||
- | {{marianas_power2.jpg?|}} | ||
- | * Turn on the power switch distributor (green) and the component power switches (red) **one at a time** | ||
- | {{marianas_power3.jpg?|}} | ||
- | * Turn on the Yokogawa spinning disk scanner (using the key) | ||
- | {{marianas_power4.jpg?|}} | ||
- | * Turn on incubation and CO2 on the touchscreen (if needed) | ||
- | {{marianas_power6.jpg?|}} | ||
- | * Turn on the lasers you need to use (using the key and the power switches) | ||
- | {{marianas_power7.jpg?|}} | ||
- | * Turn on the computer | ||
- | * Wait 2 min to allow for system components detection | ||
- | * Start the **SlideBook** software | ||
- | |||
- | **Warning**: If you need to change the stage adaptor, please contact the <html><a href="/facility/bioimaging/doku.php?id=about">Bioimaging Facility</a> (<b>imm-bioimaging@medicina.ulisboa.pt</b> | <img src="/facility/bioimaging/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47316</html>) | ||
- | |||
- | ===== Microscope Turn Off Procedure ===== | ||
- | * Remove your sample from the stage/piezo holder | ||
- | * Exit the **SlideBook** software | ||
- | * Switch off the computer | ||
- | * Turn off all system components (follow the Turn On Procedure in reverse) | ||
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- | ---- | ||
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- | [[resources|Back to the Resources page]] |