zeiss_cell_observer_sd
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zeiss_cell_observer_sd [2016/07/01 11:40] bioimaging |
zeiss_cell_observer_sd [2016/07/01 15:20] bioimaging |
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| - | <a href="/facility/bioimaging/lib/exe/fetch.php?media=3i_marianas_sdc_wiki.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=3i_marianas_sdc_wiki.jpg" width=300></a> | + | <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_sd_wiki.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_sd_wiki.jpg" width=300></a> |
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| <b>Location</b>: Room P2-A-22 (<img src="/facility/bioimaging/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47217) <br> | <b>Location</b>: Room P2-A-22 (<img src="/facility/bioimaging/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47217) <br> | ||
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| | 40x | [[https://www.micro-shop.zeiss.com/?s=42373720ae8af6&l=en&p=us&f=o&a=v&m=s&id=420762-9900-000|Plan-Apochromat]] | Oil | 1.4 | 0.13 | | | 40x | [[https://www.micro-shop.zeiss.com/?s=42373720ae8af6&l=en&p=us&f=o&a=v&m=s&id=420762-9900-000|Plan-Apochromat]] | Oil | 1.4 | 0.13 | | ||
| | 63x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=420782-9900-000|Plan-Apochromat]] | Oil | 1.40 | 0.19 | | | 63x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=420782-9900-000|Plan-Apochromat]] | Oil | 1.40 | 0.19 | | ||
| - | | 100x | [[https://www.micro-shop.zeiss.com/?s=129353259d52f12&l=en&p=uk&f=o&a=v&m=s&id=420790-9901-000|Plan-Apochromat]] | Oil | 1.40 | 0.17 | | + | | 100x | [[https://www.micro-shop.zeiss.com/?s=7138707e1a1de&l=en&p=uk&f=o&a=v&m=s&id=420792-9800-000|alpha Plan-Apochromat]] | Oil | 1.46 | 0.11 | |
| - | **Upon request:** | + | ==== Filtersets (Widefield) ==== |
| - | ^ Magnification ^ Model ^ Type ^ NA ^ WD (mm) ^ | + | |
| - | | 25x | [[https://www.micro-shop.zeiss.com/?s=129353259d52f12&l=en&p=uk&f=o&a=v&m=s&id=420852-9972-000|LCI Plan-NeoFluar]] | Oil/Glyc/W | 0.8 | 0.21 | | + | |
| - | | 40x | [[https://www.micro-shop.zeiss.com/?s=129353259d52f12&l=en&p=uk&f=o&a=v&m=s&id=420660-9970-000|Plan-Apochromat]] | Dry | 0.95 | 0.25 | | + | |
| - | ==== Emission Filtersets ==== | + | |
| - | ^ Setting ^ Transmission ^ | + | |
| - | | 1: 445 | 422-477 nm | | + | |
| - | | 2: 525 | 510-540 nm | | + | |
| - | | 3: 617 | 580-653 nm | | + | |
| - | | 4: 692 | 672-712 nm | | + | |
| - | | 5: QUAD | 440+521+607+700 nm | | + | |
| - | | 6: Block | No Transmission | | + | |
| - | | 7: Block | No Transmission | | + | |
| - | | 8: Block | No Transmission | | + | |
| - | | 9: Empty | Empty | | + | |
| - | | 10: 445 | 422-477 nm | | + | |
| - | ===== Microscope Turn On Procedure ===== | + | ^ Position ^ Filterset ^ Reference ^ Excitation ^ Emission ^ |
| - | * Check that the main power supply switch is on (should be on by default) | + | | 1 | Green | [[https://www.micro-shop.zeiss.com/?s=7139069fdc79c&l=en&p=us&f=f&a=v&b=f&id=489038-9901-000&o=|FS38HE]] | 450-490 nm | 500-550 nm | |
| - | {{marianas_power1.jpg?|}} | + | | 2 | Red | [[https://www.micro-shop.zeiss.com/?s=4237481102cf7b&l=en&p=us&f=f&a=v&b=f&id=489043-9901-000&o=|FS43HE]] | 538-562 nm | 570-640 nm | |
| - | * Turn on the fluorescence lamp (if needed) | + | | 3 | Blue | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=488049-9901-000&o=|FS49]] | 335-383 nm | 420-470 nm | |
| - | {{marianas_power5.jpg?|}} | + | | 4 | Far Red | [[https://www.micro-shop.zeiss.com/?s=4237481102cf7b&l=en&p=us&f=f&a=v&b=f&id=488050-9901-000&o=|FS50]] | 625-655 nm | 665-715 nm | |
| - | * Turn on the secondary power supply switch | + | | 5 | QUAD | [[https://www.micro-shop.zeiss.com/?s=4237481102cf7b&l=en&p=us&f=f&a=v&b=f&id=489081-9010-000&o=| FS81HE]] | 357-393 nm | 407-412 nm | |
| - | {{marianas_power2.jpg?|}} | + | | ::: | ::: | ::: | 473-496 nm | 511-528 nm | |
| - | * Turn on the power switch distributor (green) and the component power switches (red) **one at a time** | + | | ::: | ::: | ::: | 545-562 nm | 577-602 nm | |
| - | {{marianas_power3.jpg?|}} | + | | ::: | ::: | ::: | 620-642 nm | 658-704 nm | |
| - | * Turn on the Yokogawa spinning disk scanner (using the key) | + | | 6 | none | | | | |
| - | {{marianas_power4.jpg?|}} | + | |
| - | * Turn on incubation and CO2 on the touchscreen (if needed) | + | |
| - | {{marianas_power6.jpg?|}} | + | |
| - | * Turn on the lasers you need to use (using the key and the power switches) | + | |
| - | {{marianas_power7.jpg?|}} | + | |
| - | * Turn on the computer | + | |
| - | * Wait 2 min to allow for system components detection | + | |
| - | * Start the **SlideBook** software | + | |
| - | **Warning**: If you need to change the stage adaptor, please contact the <html><a href="/facility/bioimaging/doku.php?id=about">Bioimaging Facility</a> (<b>imm-bioimaging@medicina.ulisboa.pt</b> | <img src="/facility/bioimaging/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47316</html>) | + | ==== Emission Filtersets (Confocal) ==== |
| - | + | ^ Position ^ Transmission ^ | |
| - | ===== Microscope Turn Off Procedure ===== | + | | 1: BP 450/50 | 425-475 nm | |
| - | * Remove your sample from the stage/piezo holder | + | | 2: TBP 526 601 688 | 510-542 nm | |
| - | * Exit the **SlideBook** software | + | | ::: | 586-616 nm | |
| - | * Switch off the computer | + | | ::: | 663-713 nm | |
| - | * Turn off all system components (follow the Turn On Procedure in reverse) | + | | 3: none | | |
| - | + | | 4: FE01-520/35 | 502-537 nm | | |
| - | ---- | + | | 5: BP 600 50 | 575-625 nm | |
| - | + | | 6: BP 690/50 | 665-715 nm | | |
| - | [[resources|Back to the Resources page]] | + | |
zeiss_cell_observer_sd.txt · Last modified: 2024/04/01 23:53 by bioimaging
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