zeiss_cell_observer_sd
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zeiss_cell_observer_sd [2016/07/01 11:15] bioimaging created |
zeiss_cell_observer_sd [2016/07/01 13:59] bioimaging [Emission Filtersets (Confocal)] |
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| - | <img src="/facility/bioimaging/lib/exe/fetch.php?media=3i_marianas_sdc_name.jpg"> | + | <img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_sd_name.jpg"> |
| <table style="border:0px solid white;"> | <table style="border:0px solid white;"> | ||
| <tr style="border:0px solid white;"> | <tr style="border:0px solid white;"> | ||
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| <a href="/facility/bioimaging/lib/exe/fetch.php?media=3i_marianas_sdc_wiki.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=3i_marianas_sdc_wiki.jpg" width=300></a> | <a href="/facility/bioimaging/lib/exe/fetch.php?media=3i_marianas_sdc_wiki.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=3i_marianas_sdc_wiki.jpg" width=300></a> | ||
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| - | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=3i_marianas_sdc_map.jpg">Room P2-A-22</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47217) <br> | + | <b>Location</b>: Room P2-A-22 (<img src="/facility/bioimaging/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47217) <br> |
| - | <b>Manufacturer</b>: <a href="https://www.intelligent-imaging.com/" target="_blank">Intelligent Imaging Innovations</a><br> | + | <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">Carl Zeiss Microimaging</a><br> |
| - | <b>Model</b>: <a href="https://www.intelligent-imaging.com/marianas.php" target="_blank">Marianas</a><br> | + | <b>Model</b>: <a href="http://www.zeiss.com/microscopy/en_de/products/confocal-microscopes/cell-observer-sd-for-biology.html" target="_blank">Cell Observer SD</a><br> |
| - | <b>Software</b>: SlideBook 6.0.4<br> | + | <b>Software</b>: ZEN<br> |
| - | <b>Year</b>: 2012 <br> | + | <b>Year</b>: 2015 <br> |
| - | <b>SN</b>: 1648 <br> | + | |
|   <br> |   <br> | ||
|   <br> |   <br> | ||
|   <br> |   <br> | ||
|   <br> |   <br> | ||
| - | → <a href="https://imm.medicina.ulisboa.pt/intranet/booking/111"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> 3i Marianas SDC Booking</a> | + |   <br> |
| + |   <br> | ||
| + |   <br> | ||
| + | → <a href="https://imm.medicina.ulisboa.pt/intranet/booking/119"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> Zeiss Cell Observer SD Booking</a> | ||
| <br> | <br> | ||
| - | → <a href="/facility/bioimaging/doku.php?id=3i_marianas_sdc_quality"><img src="/facility/bioimaging/lib/exe/fetch.php?media=list-icon.png" width=18> 3i Marianas SDC Quality Control</a> | ||
| - | <br> | ||
| - | → <a href="/facility/bioimaging/doku.php?id=3i_marianas_sdc_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> 3i Marianas SDC Usage Statistics</a> | ||
| - |   </p> | ||
| </td> | </td> | ||
| </tr> | </tr> | ||
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| - | {{ :spinning_disk.jpg?0x300|}} The 3i Marianas SDC //spinning disk confocal microscope// is a fast imaging system which provides a trade-off between confocality, resolution and speed. It is an inverted microscope ideal for live cell applications which require fast acquisition speeds rather than high resolution images. The scanning unit achieves confocality by directing light through a spinning disk with many small pinholes. Images are then acquired with a sensitive EMCCD which allows for very small exposure times but is limited in resolution to 512x512 pixels. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions. The system is also equipped with a large size incubator for temperature control and a small stage incubator for CO2 supply. With this system you can perform optical sectioning of fluorescent samples which are too thick for a widefield system such as the [[zeiss_axio_observer|Zeiss Axio Observer]]. Even though its resolution is not as high as a //point-scanning confocal system// such as the [[zeiss_lsm_880|Zeiss LSM 880]] or [[zeiss_lsm_710|Zeiss LSM 710]], it is much faster than these two systems. If you need to use an upright microscope, check the [[zeiss_lsm_7_live|Zeiss LSM 7 Live]] fast confocal system instead. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]]. | + | {{ :spinning_disk.jpg?0x300|}} The Zeiss Cell Observer SD //spinning disk confocal microscope// is a fast imaging system which provides a trade-off between confocality, resolution and speed. It is an inverted microscope ideal for live cell applications which require fast acquisition speeds rather than high resolution images. The scanning unit achieves confocality by directing light through a spinning disk with many small pinholes. Images are then acquired with a sensitive EMCCD which allows for very small exposure times but is limited in resolution to 512x512 pixels. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions. The system is also equipped with a large size incubator for temperature control, a small stage incubator for CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. You can also use the system as a widefield microscope, using a sCMOS camera for acquisition. |
| * **Microscope**: [[http://www.zeiss.com/microscopy/en_de/products/light-microscopes/axio-observer-for-biology.html|Zeiss Axio Observer]] | * **Microscope**: [[http://www.zeiss.com/microscopy/en_de/products/light-microscopes/axio-observer-for-biology.html|Zeiss Axio Observer]] | ||
| * **Confocal scanner**: [[http://www.andor.com/microscopy_systems/peripherals/confocal_scanners/|Yokogawa CSU-x1]] | * **Confocal scanner**: [[http://www.andor.com/microscopy_systems/peripherals/confocal_scanners/|Yokogawa CSU-x1]] | ||
| - | * **Camera**: [[http://www.photometrics.com/products/emccdcams/evolve/512.php|Evolve 512 EMCCD]] | + | * **EMCCD Camera**: [[http://www.photometrics.com/products/emccdcams/evolve/512.php|Evolve 512 EMCCD]] |
| + | * **sCMOS Camera**: [[http://www.hamamatsu.com/eu/en/community/life_science_camera/product/search/C11440-22CU/index.html|Hamamatsu ORCA-flash4.0 V2]] | ||
| ===== System components ===== | ===== System components ===== | ||
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| ==== LASERs ==== | ==== LASERs ==== | ||
| ^ Laser Unit ^ Wavelength ^ Maximum Power ^ Current Status ^ | ^ Laser Unit ^ Wavelength ^ Maximum Power ^ Current Status ^ | ||
| - | | Laserstack 488 | 488 nm | 100 mW | ok | | + | | Solid State Laser 405 | 405 nm | 50 mW | ok | |
| - | | Laserstack 561 | 561 nm | 100 mW | ok | | + | | Solid State Laser 488 | 488 nm | 100 mW | ok | |
| - | | Laserstack 640 | 640 nm | 100 mW | ok | | + | | Solid State Laser 561 | 561 nm | 75 mW | ok | |
| + | | Solid State Laser 638 | 638 nm | 75 mW | ok | | ||
| ==== Objectives ==== | ==== Objectives ==== | ||
| ^ Magnification ^ Model ^ Type ^ NA ^ WD (mm) ^ | ^ Magnification ^ Model ^ Type ^ NA ^ WD (mm) ^ | ||
| - | | 20x | [[https://www.micro-shop.zeiss.com/?s=129353259d52f12&l=en&p=uk&f=o&a=v&m=s&id=420650-9901-000|Plan-Apochromat]] | Dry | 0.8 | 0.55 | | + | | 20x | [[https://www.micro-shop.zeiss.com/?s=42373720ae8af6&l=en&p=us&f=o&a=v&m=s&id=420651-9911-000|Plan-Apochromat]] | Dry | 0.8 | 0.55 | |
| + | | 40x | [[https://www.micro-shop.zeiss.com/?s=42373720ae8af6&l=en&p=us&f=o&a=v&m=s&id=421867-9970-000|LD C-Apochromat]] | Water | 1.1 | 0.62 | | ||
| + | | 40x | [[https://www.micro-shop.zeiss.com/?s=42373720ae8af6&l=en&p=us&f=o&a=v&m=s&id=420762-9900-000|Plan-Apochromat]] | Oil | 1.4 | 0.13 | | ||
| | 63x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=420782-9900-000|Plan-Apochromat]] | Oil | 1.40 | 0.19 | | | 63x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=420782-9900-000|Plan-Apochromat]] | Oil | 1.40 | 0.19 | | ||
| - | | 100x | [[https://www.micro-shop.zeiss.com/?s=129353259d52f12&l=en&p=uk&f=o&a=v&m=s&id=420790-9901-000|Plan-Apochromat]] | Oil | 1.40 | 0.17 | | + | | 100x | [[https://www.micro-shop.zeiss.com/?s=7138707e1a1de&l=en&p=uk&f=o&a=v&m=s&id=420792-9800-000|alpha Plan-Apochromat]] | Oil | 1.46 | 0.11 | |
| + | |||
| + | ==== Filtersets (Widefield) ==== | ||
| + | |||
| + | ^ Position ^ Filterset ^ Reference ^ Excitation ^ Emission ^ | ||
| + | | 1 | Green | [[https://www.micro-shop.zeiss.com/?s=7139069fdc79c&l=en&p=us&f=f&a=v&b=f&id=489038-9901-000&o=|FS38HE]] | 450-490 nm | 500-550 nm | | ||
| + | | 2 | Red | [[https://www.micro-shop.zeiss.com/?s=4237481102cf7b&l=en&p=us&f=f&a=v&b=f&id=489043-9901-000&o=|FS43HE]] | 538-562 nm | 570-640 nm | | ||
| + | | 3 | Blue | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=488049-9901-000&o=|FS49]] | 335-383 nm | 420-470 nm | | ||
| + | | 4 | Far Red | [[https://www.micro-shop.zeiss.com/?s=4237481102cf7b&l=en&p=us&f=f&a=v&b=f&id=488050-9901-000&o=|FS50]] | 625-655 nm | 665-715 nm | | ||
| + | | 5 | QUAD | [[https://www.micro-shop.zeiss.com/?s=4237481102cf7b&l=en&p=us&f=f&a=v&b=f&id=489081-9010-000&o=| FS81HE]] | 357-393 nm | 407-412 nm | | ||
| + | | ::: | ::: | ::: | 473-496 nm | 511-528 nm | | ||
| + | | ::: | ::: | ::: | 545-562 nm | 577-602 nm | | ||
| + | | ::: | ::: | ::: | 620-642 nm | 658-704 nm | | ||
| + | | 6 | none | | | | | ||
| - | **Upon request:** | + | ==== Emission Filtersets (Confocal) ==== |
| - | ^ Magnification ^ Model ^ Type ^ NA ^ WD (mm) ^ | + | ^ Position ^ Transmission ^ |
| - | | 25x | [[https://www.micro-shop.zeiss.com/?s=129353259d52f12&l=en&p=uk&f=o&a=v&m=s&id=420852-9972-000|LCI Plan-NeoFluar]] | Oil/Glyc/W | 0.8 | 0.21 | | + | | 1: BP 450/50 | 425-475 nm | |
| - | | 40x | [[https://www.micro-shop.zeiss.com/?s=129353259d52f12&l=en&p=uk&f=o&a=v&m=s&id=420660-9970-000|Plan-Apochromat]] | Dry | 0.95 | 0.25 | | + | | 2: TBP 526 601 688 | 510-542 nm | |
| - | ==== Emission Filtersets ==== | + | | ::: | 586-616 nm | |
| - | ^ Setting ^ Transmission ^ | + | | ::: | 663-713 nm | |
| - | | 1: 445 | 422-477 nm | | + | | 3: none | | |
| - | | 2: 525 | 510-540 nm | | + | | 4: FE01-520/35 | 502-537 nm | |
| - | | 3: 617 | 580-653 nm | | + | | 5: BP 600 50 | 575-625 nm | |
| - | | 4: 692 | 672-712 nm | | + | | 6: BP 690/50 | 665-715 nm | |
| - | | 5: QUAD | 440+521+607+700 nm | | + | |
| - | | 6: Block | No Transmission | | + | |
| - | | 7: Block | No Transmission | | + | |
| - | | 8: Block | No Transmission | | + | |
| - | | 9: Empty | Empty | | + | |
| - | | 10: 445 | 422-477 nm | | + | |
| ===== Microscope Turn On Procedure ===== | ===== Microscope Turn On Procedure ===== | ||
zeiss_cell_observer_sd.txt · Last modified: 2024/04/01 23:53 by bioimaging
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