zeiss_cell_observer
Differences
This shows you the differences between two versions of the page.
| Both sides previous revision Previous revision Next revision | Previous revision Next revision Both sides next revision | ||
|
zeiss_cell_observer [2021/01/17 04:44] bioimaging [Microscope Turn Off procedures] |
zeiss_cell_observer [2022/01/20 13:26] bioimaging [Microscope Turn Off procedures] |
||
|---|---|---|---|
| Line 6: | Line 6: | ||
| <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_wiki_new.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_wiki_new.png" width=300></a> | <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_wiki_new.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_wiki_new.png" width=300></a> | ||
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| - | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_map_new.png">Room P3-A-10</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47331) <br> | + | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_map_new_p2a24.png">Room P2-A-24</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47219) <br> |
| <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">Carl Zeiss MicroImaging</a><br> | <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">Carl Zeiss MicroImaging</a><br> | ||
| <b>Model</b>: Axio Observer<br> | <b>Model</b>: Axio Observer<br> | ||
| <b>Nickname</b>: "Cell Observer"<br> | <b>Nickname</b>: "Cell Observer"<br> | ||
| - | <b>Software</b>: ZEN 2 (blue edition)<br> | + | <b>Software</b>: ZEN 3.0 (blue edition)<br> |
| <b>Year</b>: 2015 <br> | <b>Year</b>: 2015 <br> | ||
| <b>SN</b>: 3851002396<br> | <b>SN</b>: 3851002396<br> | ||
| Line 59: | Line 59: | ||
| ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ||
| | 10x | EC Plan-Neofluar Ph1 | Air | 0.30 | 5.20 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420341-9911-000/Objective-EC-Plan-Neofluar-10x-0.30-Ph1-M27|420341-9911-000]] | | | 10x | EC Plan-Neofluar Ph1 | Air | 0.30 | 5.20 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420341-9911-000/Objective-EC-Plan-Neofluar-10x-0.30-Ph1-M27|420341-9911-000]] | | ||
| - | | 20x | EC Plan-Neofluar Ph 2 | Air | 0.50 | 2.00 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/440341-9904-000/Objective-EC-Plan-Neofluar-20x-0.5-Ph2|440341-9904-000]] | | ||
| | 20x | Plan-Apochromat | Air | 0.80 | 0.55 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420650-9901-000/Objective-Plan-Apochromat-20x-0.8-M27|420650-9901-000]] | | | 20x | Plan-Apochromat | Air | 0.80 | 0.55 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420650-9901-000/Objective-Plan-Apochromat-20x-0.8-M27|420650-9901-000]] | | ||
| + | | 20x | EC Plan-Neofluar Ph 2 | Air | 0.50 | 2.00 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/440341-9904-000/Objective-EC-Plan-Neofluar-20x-0.5-Ph2|440341-9904-000]] | | ||
| | 40x | EC Plan-NeoFluar | Air | 0.75 | 0.71 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420360-9900-000/Objective-EC-Plan-Neofluar-40x-0.75-M27|420360-9900-000]] | | | 40x | EC Plan-NeoFluar | Air | 0.75 | 0.71 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420360-9900-000/Objective-EC-Plan-Neofluar-40x-0.75-M27|420360-9900-000]] | | ||
| | 63x | Plan-Apochromat DIC | Oil | 1.40 | 0.19 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420782-9900-000/Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-000]] | | | 63x | Plan-Apochromat DIC | Oil | 1.40 | 0.19 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420782-9900-000/Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-000]] | | ||
| Line 77: | Line 77: | ||
| ^ Model ^ Type ^ Frame Size ^ Pixel Size (µm) ^ | ^ Model ^ Type ^ Frame Size ^ Pixel Size (µm) ^ | ||
| | [[https://www.zeiss.com/microscopy/int/products/microscope-cameras/axiocam-506-mono.html|Axiocam 506 mono]] | CCD | 2752 x 2208 | 4.54 x 4.54 | | | [[https://www.zeiss.com/microscopy/int/products/microscope-cameras/axiocam-506-mono.html|Axiocam 506 mono]] | CCD | 2752 x 2208 | 4.54 x 4.54 | | ||
| + | |||
| ===== System Turn On Procedures ===== | ===== System Turn On Procedures ===== | ||
| - | * Turn on the HXP 120 fluorescent light source | + | |
| + | * Turn on the HXP 120 fluorescent light source (if used) | ||
| {{zeiss_axio_observer_hxp.jpg?120|}} | {{zeiss_axio_observer_hxp.jpg?120|}} | ||
| - | * Turn on the temperature and CO2 controller (if you need them). | + | * Turn on the temperature and CO2 controller - white power strip (if you need them). |
| - | {{zeiss_axio_observer_temp.jpg?80|}} | + | * If you need CO2, open the Cell Observer CO2 valve |
| - | * If you need CO2, open the main valve and the Cell Observer CO2 valve | + | |
| - | {{::p3a10_co2_valves.png?200|}} | + | |
| * Turn on the motorized stage controller (**A** on remote control) | * Turn on the motorized stage controller (**A** on remote control) | ||
| {{zeiss_axio_observer_a.jpg?height=150|}} | {{zeiss_axio_observer_a.jpg?height=150|}} | ||
| - | * Turn on the Definite Focus controller (if you are going to use it) | ||
| - | {{zeiss_axio_observer_df.jpg?height=150|}} | ||
| * Turn on the microscope power supply (**B** on remote control) | * Turn on the microscope power supply (**B** on remote control) | ||
| {{zeiss_axio_observer_b.jpg?height=150|}} | {{zeiss_axio_observer_b.jpg?height=150|}} | ||
| Line 93: | Line 91: | ||
| * Log in to Windows (Biomaging User). | * Log in to Windows (Biomaging User). | ||
| * Start the **ZEN** software | * Start the **ZEN** software | ||
| - | + | * Select HXP or LED Workspace in ZEN (top left) | |
| - | **Warning**: If you need to change the stage adapter, please contact the <html><a href="/facility/bioimaging/doku.php?id=about">Bioimaging Unit</a> (<b>imm-bioimaging@medicina.ulisboa.pt</b> | <img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47222</html>) | + | {{::workspace_selection.png?250|}} |
| ===== Microscope Turn Off procedures ===== | ===== Microscope Turn Off procedures ===== | ||
| Line 106: | Line 104: | ||
| * Clean up immersion objectives | * Clean up immersion objectives | ||
| * Turn off the microscope power supply (**B** on remote control) | * Turn off the microscope power supply (**B** on remote control) | ||
| - | * Turn off the Definite Focus controller (if used) | ||
| * Turn off the motorized stage controller (**A** on remote control) | * Turn off the motorized stage controller (**A** on remote control) | ||
| - | * Turn off the Temperature and CO2 controller (if used) | + | * Turn off the Temperature and CO2 controller - white power strip (if used) |
| * Close the Cell Observer CO2 valve (if used) | * Close the Cell Observer CO2 valve (if used) | ||
| - | * Turn off the HXP 120 fluorescent light source | + | * Turn off the HXP 120 fluorescent light source (if used) |
| ---- | ---- | ||
| [[resources|Back to the Equipment page]] | [[resources|Back to the Equipment page]] | ||
zeiss_cell_observer.txt · Last modified: 2024/04/01 23:50 by bioimaging
Page Tools
