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Both sides previous revision Previous revision Next revision | Previous revision Next revision Both sides next revision | ||
zeiss_cell_observer [2020/12/02 12:52] bioimaging |
zeiss_cell_observer [2021/02/21 18:12] bioimaging [Microscope Turn Off procedures] |
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^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ||
| 10x | EC Plan-Neofluar Ph1 | Air | 0.30 | 5.20 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420341-9911-000/Objective-EC-Plan-Neofluar-10x-0.30-Ph1-M27|420341-9911-000]] | | | 10x | EC Plan-Neofluar Ph1 | Air | 0.30 | 5.20 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420341-9911-000/Objective-EC-Plan-Neofluar-10x-0.30-Ph1-M27|420341-9911-000]] | | ||
- | | 20x | EC Plan-Neofluar Ph 2 | Air | 0.50 | 2.00 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/440341-9904-000/Objective-EC-Plan-Neofluar-20x-0.5-Ph2|440341-9904-000]] | | ||
| 20x | Plan-Apochromat | Air | 0.80 | 0.55 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420650-9901-000/Objective-Plan-Apochromat-20x-0.8-M27|420650-9901-000]] | | | 20x | Plan-Apochromat | Air | 0.80 | 0.55 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420650-9901-000/Objective-Plan-Apochromat-20x-0.8-M27|420650-9901-000]] | | ||
+ | | 20x | EC Plan-Neofluar Ph 2 | Air | 0.50 | 2.00 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/440341-9904-000/Objective-EC-Plan-Neofluar-20x-0.5-Ph2|440341-9904-000]] | | ||
| 40x | EC Plan-NeoFluar | Air | 0.75 | 0.71 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420360-9900-000/Objective-EC-Plan-Neofluar-40x-0.75-M27|420360-9900-000]] | | | 40x | EC Plan-NeoFluar | Air | 0.75 | 0.71 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420360-9900-000/Objective-EC-Plan-Neofluar-40x-0.75-M27|420360-9900-000]] | | ||
| 63x | Plan-Apochromat DIC | Oil | 1.40 | 0.19 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420782-9900-000/Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-000]] | | | 63x | Plan-Apochromat DIC | Oil | 1.40 | 0.19 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420782-9900-000/Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-000]] | | ||
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^ Model ^ Type ^ Frame Size ^ Pixel Size (µm) ^ | ^ Model ^ Type ^ Frame Size ^ Pixel Size (µm) ^ | ||
| [[https://www.zeiss.com/microscopy/int/products/microscope-cameras/axiocam-506-mono.html|Axiocam 506 mono]] | CCD | 2752 x 2208 | 4.54 x 4.54 | | | [[https://www.zeiss.com/microscopy/int/products/microscope-cameras/axiocam-506-mono.html|Axiocam 506 mono]] | CCD | 2752 x 2208 | 4.54 x 4.54 | | ||
+ | |||
===== System Turn On Procedures ===== | ===== System Turn On Procedures ===== | ||
- | * Turn on the HXP 120 fluorescent light source | + | |
+ | * Select LED or HXP light source on the manual path deflecting mirror (in the back of the microscope) | ||
+ | {{:cell_observer_led_selected.png?150|}} {{:cell_observer_hxp_selected.png?150|}} | ||
+ | * Turn on the HXP 120 fluorescent light source (if used) | ||
{{zeiss_axio_observer_hxp.jpg?120|}} | {{zeiss_axio_observer_hxp.jpg?120|}} | ||
* Turn on the temperature and CO2 controller (if you need them). | * Turn on the temperature and CO2 controller (if you need them). | ||
{{zeiss_axio_observer_temp.jpg?80|}} | {{zeiss_axio_observer_temp.jpg?80|}} | ||
- | * If you need CO2, open the valve | + | * If you need CO2, open the main valve and the Cell Observer CO2 valve |
- | {{zeiss_axio_observer_co2.jpg?80|}} | + | {{::p3a10_co2_valves.png?200|}} |
* Turn on the motorized stage controller (**A** on remote control) | * Turn on the motorized stage controller (**A** on remote control) | ||
{{zeiss_axio_observer_a.jpg?height=150|}} | {{zeiss_axio_observer_a.jpg?height=150|}} | ||
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* Log in to Windows (Biomaging User). | * Log in to Windows (Biomaging User). | ||
* Start the **ZEN** software | * Start the **ZEN** software | ||
+ | * Select HXP or LED Workspace in ZEN (top left) | ||
+ | {{::workspace_selection.png?250|}} | ||
**Warning**: If you need to change the stage adapter, please contact the <html><a href="/facility/bioimaging/doku.php?id=about">Bioimaging Unit</a> (<b>imm-bioimaging@medicina.ulisboa.pt</b> | <img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47222</html>) | **Warning**: If you need to change the stage adapter, please contact the <html><a href="/facility/bioimaging/doku.php?id=about">Bioimaging Unit</a> (<b>imm-bioimaging@medicina.ulisboa.pt</b> | <img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47222</html>) | ||
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* Turn off the motorized stage controller (**A** on remote control) | * Turn off the motorized stage controller (**A** on remote control) | ||
* Turn off the Temperature and CO2 controller (if used) | * Turn off the Temperature and CO2 controller (if used) | ||
- | * Turn off the HXP 120 fluorescent light source | + | * Close the Cell Observer CO2 valve (if used) |
+ | * Turn off the HXP 120 fluorescent light source (if used) | ||
---- | ---- | ||
- | [[resources|Back to the Resources page]] | + | [[resources|Back to the Equipment page]] |