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zeiss_cell_observer [2020/01/01 20:45] bioimaging [Objectives] |
zeiss_cell_observer [2020/01/17 12:33] bioimaging [Microscope overview] |
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===== Microscope overview ===== | ===== Microscope overview ===== | ||
- | The Zeiss Cell Observer is a fully motorized inverted //widefield fluorescence microscope// ideal for live-cell imaging applications. It is equipped with a large cage incubator and a small stage incubator for temperature control and CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. Using the ZEN 2 software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. Its sensitive cooled Axiocam 506m camera allows you to capture weak fluorescent signals and minimize photobleaching/photodamage in light sensitive samples. You can also perform Z-stack acquisition and use [[https://huygens.i3s.up.pt/login.php|PPBI Huygens RM]] [[http://micro.magnet.fsu.edu/primer/digitalimaging/deconvolution/deconvolutionhome.html|deconvolution]] server to significantly improve image resolution even in thick samples. If don't want to use deconvolution, check out one of the confocal systems such as the point scanners [[zeiss_lsm_710|Zeiss LSM 710]] and [[zeiss_lsm_880|Zeiss LSM 880]] or spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]]. If you have FISH or other fixed fluorescence slides try the [[leica_dm5000b|Leica DM5000B]] instead. | + | {{ :widefield_lightpath.png?0x300}}The Zeiss Cell Observer is a fully motorized inverted //widefield fluorescence microscope// ideal for live-cell imaging applications. It is equipped with a large cage incubator and a small stage incubator for temperature control and CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. Using the ZEN 2 software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. Its sensitive cooled Axiocam 506m camera allows you to capture weak fluorescent signals and minimize photobleaching/photodamage in light sensitive samples. You can also perform Z-stack acquisition and use [[https://huygens.i3s.up.pt/login.php|PPBI Huygens RM]] [[http://micro.magnet.fsu.edu/primer/digitalimaging/deconvolution/deconvolutionhome.html|deconvolution]] server to significantly improve image resolution even in thick samples. If don't want to use deconvolution, check out one of the confocal systems such as the point scanners [[zeiss_lsm_710|Zeiss LSM 710]] and [[zeiss_lsm_880|Zeiss LSM 880]] or spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]]. If you have FISH or other fixed fluorescence slides try the [[leica_dm5000b|Leica DM5000B]] instead. |
* **Microscope**: [[http://www.zeiss.com/microscopy/en_de/products/light-microscopes/axio-observer-for-biology.html|Zeiss Axio Observer]] | * **Microscope**: [[http://www.zeiss.com/microscopy/en_de/products/light-microscopes/axio-observer-for-biology.html|Zeiss Axio Observer]] | ||
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==== Camera ==== | ==== Camera ==== | ||
- | ^ Model ^ Type ^ Frame Size ^ Pixel Size (µm) ^ Quantum Efficiency ^ | + | ^ Model ^ Type ^ Frame Size ^ Pixel Size (µm) ^ |
- | | [[https://www.zeiss.com/microscopy/int/products/microscope-cameras/axiocam-506-mono.html|Axiocam 506 mono CCD]] | + | | [[https://www.zeiss.com/microscopy/int/products/microscope-cameras/axiocam-506-mono.html|Axiocam 506 mono]] | CCD | 2752 x 2208 | 4.54 x 4.54 | |
- | | CCD | 512 x 512 | 16 x 16 | > 90% | | + | |
- | | [[https://www.hamamatsu.com/resources/pdf/sys/SCAS0081E_C11440-22CU.pdf|Hamamatsu ORCA-flash4.0 V2]] | sCMOS | 2048 x 2048 | 6.5 x 6.5 | 82% | | + | |
===== System Turn On Procedures ===== | ===== System Turn On Procedures ===== | ||
- | * Turn on the HXP 120 fluorescence light. | + | * Turn on the HXP 120 fluorescent light source |
{{zeiss_axio_observer_hxp.jpg?120|}} | {{zeiss_axio_observer_hxp.jpg?120|}} | ||
- | * Turn on the Temperature and CO2 controller (if you need them). | + | * Turn on the temperature and CO2 controller (if you need them). |
{{zeiss_axio_observer_temp.jpg?80|}} | {{zeiss_axio_observer_temp.jpg?80|}} | ||
* If you need CO2, open the valve | * If you need CO2, open the valve | ||
{{zeiss_axio_observer_co2.jpg?80|}} | {{zeiss_axio_observer_co2.jpg?80|}} | ||
- | * Turn on the Motorized Stage controller (A) | + | * Turn on the motorized stage controller (**A** on remote control) |
{{zeiss_axio_observer_a.jpg?height=150|}} | {{zeiss_axio_observer_a.jpg?height=150|}} | ||
* Turn on the Definite Focus controller (if you are going to use it) | * Turn on the Definite Focus controller (if you are going to use it) | ||
{{zeiss_axio_observer_df.jpg?height=150|}} | {{zeiss_axio_observer_df.jpg?height=150|}} | ||
- | * Turn on the Microscope power supply (B) | + | * Turn on the microscope power supply (**B** on remote control) |
{{zeiss_axio_observer_b.jpg?height=150|}} | {{zeiss_axio_observer_b.jpg?height=150|}} | ||
* Turn on the computer | * Turn on the computer | ||
- | * Log in Windows with Cell Observer user account. | + | * Log in to Windows (Biomaging User). |
- | * Start the **ZEN 2** software | + | * Start the **ZEN** software |
===== Microscope Turn Off procedures ===== | ===== Microscope Turn Off procedures ===== | ||
- | * Exit the **ZEN 2** software | + | If there is another user for this microscope is the __next hour__: |
+ | * Leave the fluorescent light source on | ||
+ | * Clean up immersion objectives | ||
+ | Else: | ||
+ | * Exit the ZEN software | ||
* Turn off the computer (not the UPS!) | * Turn off the computer (not the UPS!) | ||
{{:cell_observer_pc_vs_ups.png?250|}} | {{:cell_observer_pc_vs_ups.png?250|}} | ||
- | * If you used any __immersion oil in the objectives, clean them up__. | + | * Clean up immersion objectives |
- | * Turn off the microscope power supply (B) | + | * Turn off the microscope power supply (**B** on remote control) |
* Turn off the Definite Focus controller (if used) | * Turn off the Definite Focus controller (if used) | ||
- | * Turn off the Motorized Stage controller (A) | + | * Turn off the motorized stage controller (**A** on remote control) |
* Turn off the Temperature and CO2 controller (if used) | * Turn off the Temperature and CO2 controller (if used) | ||
- | * If there's __another user__ for this microscope in the __next hour__ leave the mercury __lamp power source on, else turn it off__. | + | * Turn off the HXP 120 fluorescent light source |
- | * Be extra careful in weekends and at the end of the day to make sure that the mercury lamp is not going to be turned on for an excessive time. | + | |
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[[resources|Back to the Resources page]] | [[resources|Back to the Resources page]] |