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zeiss_cell_observer [2020/01/01 20:45] bioimaging [Objectives] |
zeiss_cell_observer [2020/01/14 18:33] bioimaging [System Turn On Procedures] |
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==== Camera ==== | ==== Camera ==== | ||
- | ^ Model ^ Type ^ Frame Size ^ Pixel Size (µm) ^ Quantum Efficiency ^ | + | ^ Model ^ Type ^ Frame Size ^ Pixel Size (µm) ^ |
- | | [[https://www.zeiss.com/microscopy/int/products/microscope-cameras/axiocam-506-mono.html|Axiocam 506 mono CCD]] | + | | [[https://www.zeiss.com/microscopy/int/products/microscope-cameras/axiocam-506-mono.html|Axiocam 506 mono]] | CCD | 2752 x 2208 | 4.54 x 4.54 | |
- | | CCD | 512 x 512 | 16 x 16 | > 90% | | + | |
- | | [[https://www.hamamatsu.com/resources/pdf/sys/SCAS0081E_C11440-22CU.pdf|Hamamatsu ORCA-flash4.0 V2]] | sCMOS | 2048 x 2048 | 6.5 x 6.5 | 82% | | + | |
===== System Turn On Procedures ===== | ===== System Turn On Procedures ===== | ||
- | * Turn on the HXP 120 fluorescence light. | + | * Turn on the HXP 120 fluorescent light source |
{{zeiss_axio_observer_hxp.jpg?120|}} | {{zeiss_axio_observer_hxp.jpg?120|}} | ||
- | * Turn on the Temperature and CO2 controller (if you need them). | + | * Turn on the temperature and CO2 controller (if you need them). |
{{zeiss_axio_observer_temp.jpg?80|}} | {{zeiss_axio_observer_temp.jpg?80|}} | ||
* If you need CO2, open the valve | * If you need CO2, open the valve | ||
{{zeiss_axio_observer_co2.jpg?80|}} | {{zeiss_axio_observer_co2.jpg?80|}} | ||
- | * Turn on the Motorized Stage controller (A) | + | * Turn on the motorized stage controller (**A** on remote control) |
{{zeiss_axio_observer_a.jpg?height=150|}} | {{zeiss_axio_observer_a.jpg?height=150|}} | ||
* Turn on the Definite Focus controller (if you are going to use it) | * Turn on the Definite Focus controller (if you are going to use it) | ||
{{zeiss_axio_observer_df.jpg?height=150|}} | {{zeiss_axio_observer_df.jpg?height=150|}} | ||
- | * Turn on the Microscope power supply (B) | + | * Turn on the microscope power supply (**B** on remote control) |
{{zeiss_axio_observer_b.jpg?height=150|}} | {{zeiss_axio_observer_b.jpg?height=150|}} | ||
* Turn on the computer | * Turn on the computer | ||
- | * Log in Windows with Cell Observer user account. | + | * Log in to Windows (Biomaging User). |
- | * Start the **ZEN 2** software | + | * Start the **ZEN** software |
===== Microscope Turn Off procedures ===== | ===== Microscope Turn Off procedures ===== |