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zeiss_cell_observer [2020/01/01 20:31] bioimaging [Objectives] |
zeiss_cell_observer [2020/01/14 18:40] bioimaging [Microscope Turn Off procedures] |
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| 100x | Plan-Apochromat DIC | Oil | 1.40 | 0.17 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/440782-9902-000/Objective-Plan-Apochromat-100x-1.40-Oil-DIC|440782-9902-000]] | | | 100x | Plan-Apochromat DIC | Oil | 1.40 | 0.17 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/440782-9902-000/Objective-Plan-Apochromat-100x-1.40-Oil-DIC|440782-9902-000]] | | ||
+ | ==== Camera ==== | ||
+ | ^ Model ^ Type ^ Frame Size ^ Pixel Size (µm) ^ | ||
+ | | [[https://www.zeiss.com/microscopy/int/products/microscope-cameras/axiocam-506-mono.html|Axiocam 506 mono]] | CCD | 2752 x 2208 | 4.54 x 4.54 | | ||
===== System Turn On Procedures ===== | ===== System Turn On Procedures ===== | ||
- | * Turn on the HXP 120 fluorescence light. | + | * Turn on the HXP 120 fluorescent light source |
{{zeiss_axio_observer_hxp.jpg?120|}} | {{zeiss_axio_observer_hxp.jpg?120|}} | ||
- | * Turn on the Temperature and CO2 controller (if you need them). | + | * Turn on the temperature and CO2 controller (if you need them). |
{{zeiss_axio_observer_temp.jpg?80|}} | {{zeiss_axio_observer_temp.jpg?80|}} | ||
* If you need CO2, open the valve | * If you need CO2, open the valve | ||
{{zeiss_axio_observer_co2.jpg?80|}} | {{zeiss_axio_observer_co2.jpg?80|}} | ||
- | * Turn on the Motorized Stage controller (A) | + | * Turn on the motorized stage controller (**A** on remote control) |
{{zeiss_axio_observer_a.jpg?height=150|}} | {{zeiss_axio_observer_a.jpg?height=150|}} | ||
* Turn on the Definite Focus controller (if you are going to use it) | * Turn on the Definite Focus controller (if you are going to use it) | ||
{{zeiss_axio_observer_df.jpg?height=150|}} | {{zeiss_axio_observer_df.jpg?height=150|}} | ||
- | * Turn on the Microscope power supply (B) | + | * Turn on the microscope power supply (**B** on remote control) |
{{zeiss_axio_observer_b.jpg?height=150|}} | {{zeiss_axio_observer_b.jpg?height=150|}} | ||
* Turn on the computer | * Turn on the computer | ||
- | * Log in Windows with Cell Observer user account. | + | * Log in to Windows (Biomaging User). |
- | * Start the **ZEN 2** software | + | * Start the **ZEN** software |
===== Microscope Turn Off procedures ===== | ===== Microscope Turn Off procedures ===== | ||
- | * Exit the **ZEN 2** software | + | If there is another user for this microscope is the __next hour__: |
+ | * Leave the fluorescent light source on | ||
+ | * Clean up immersion objectives | ||
+ | Else: | ||
+ | * Exit the ZEN software | ||
* Turn off the computer (not the UPS!) | * Turn off the computer (not the UPS!) | ||
{{:cell_observer_pc_vs_ups.png?250|}} | {{:cell_observer_pc_vs_ups.png?250|}} | ||
- | * If you used any __immersion oil in the objectives, clean them up__. | + | * Clean up immersion objectives |
- | * Turn off the microscope power supply (B) | + | * Turn off the microscope power supply (**B** on remote control) |
* Turn off the Definite Focus controller (if used) | * Turn off the Definite Focus controller (if used) | ||
- | * Turn off the Motorized Stage controller (A) | + | * Turn off the motorized stage controller (**A** on remote control) |
* Turn off the Temperature and CO2 controller (if used) | * Turn off the Temperature and CO2 controller (if used) | ||
- | * If there's __another user__ for this microscope in the __next hour__ leave the mercury __lamp power source on, else turn it off__. | + | * Turn off the HXP 120 fluorescent light source |
- | * Be extra careful in weekends and at the end of the day to make sure that the mercury lamp is not going to be turned on for an excessive time. | + | |
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[[resources|Back to the Resources page]] | [[resources|Back to the Resources page]] |