User Tools

Site Tools


zeiss_cell_observer

Differences

This shows you the differences between two versions of the page.

Link to this comparison view

Both sides previous revision Previous revision
Next revision
Previous revision
Next revision Both sides next revision
zeiss_cell_observer [2020/01/01 20:11]
bioimaging [Microscope overview]
zeiss_cell_observer [2020/01/01 20:45]
bioimaging [Objectives]
Line 59: Line 59:
  
 ==== Objectives ==== ==== Objectives ====
-^  Magnification ​ ^  Model  ^  ​Type  ​^ ​ NA  ^  WD (mm)  ^ +^  Magnification ​ ^  Model  ^  ​Immersion ​ ​^ ​ NA  ^  WD (mm)  ​^ ​ Reference ​ ^ 
-|  10x  |  [[https://​www.micro-shop.zeiss.com/​?​s=3407786617d462&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=420340-9901-000|EC Plan-Neofluar]] ​ |  ​Dry  ​|  ​0.30  ​|  ​5. +|  10x  ​| ​ EC Plan-Neofluar ​ |  Air  |  0.30  |  5.2  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420340-9901-000/Objective-EC-Plan-Neofluar-10x-0.30-M27|420340-9901-000]]  ​
-|  ​20x  ​| ​ [[https://​www.micro-shop.zeiss.com/​?​s=2310860526ae9cb&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=420650-9901-000|Plan-Apochromat]] ​ |  ​Dry  ​|  ​0.80  ​| ​ 0.55  +|  ​20x  ​|  ​Plan-Apochromat ​ ​|  ​Air  |  0.80  ​|  ​0.55  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420650-9901-000/Objective-Plan-Apochromat-20x-0.8-M27|420650-9901-000]]  ​
-|  ​40x  ​| ​ [[https://​www.micro-shop.zeiss.com/​?​s=3407786617d462&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=440350-9903-000|EC Plan-NeoFluar]]  |  ​Dry  ​|  ​0.75  ​|  ​0.71  +|  ​40x  ​|  ​EC Plan-NeoFluar ​ |  Air  ​| ​ 0.75  ​|  ​0.71  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​440350-9903-000/Objective-EC-Plan-Neofluar-40x-0.75|440350-9903-000]]  ​
-|  ​63x  ​| ​ [[https://​www.micro-shop.zeiss.com/​?​s=3407786617d462&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=420782-9900-000|Plan-Apochromat]]  |  Oil  |  ​1.40  ​ ​0.19 ​ |+|  ​63x  ​|  ​Plan-Apochromat DIC  ​|  ​Oil  |  1.40  ​|  ​0.19  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420782-9900-000/Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-000]] ​ |
  
 **Upon request:** **Upon request:**
-^  Magnification ​ ^  Model  ^  ​Type  ​^ ​ NA  ^  WD (mm)  ^ +^  Magnification ​ ^  Model  ^  ​Immersion ​ ​^ ​ NA  ^  WD (mm)  ​^ ​ Reference ​ ^ 
-|  1.25x  |  [[https://​www.micro-shop.zeiss.com/​?​s=3407786617d462&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=422310-9900-000|EC Epiplan-Neofluar]] ​ |  ​Dry  ​|  ​0.035  ​|  ​ | +|  1.25x  ​| ​ EC Epiplan-Neofluar ​ |  Air  |  0.035  |  4  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​422310-9900-000/Objective-EC-Epiplan-Neofluar-1.25x-0.03-M27|422310-9900-000]]  ​
-|  ​5x  ​| ​ [[https://​www.micro-shop.zeiss.com/​?​s=571974962233f6&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=420330-9901-000|EC Plan-NeoFluar]]  |  ​Dry  ​|  ​0.16  ​|  ​18.5  | +|  ​5x  ​|  ​EC Plan-NeoFluar ​ ​|  ​Air  ​| ​ ​0.16  ​|  ​18.5  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420330-9901-000/Objective-EC-Plan-Neofluar-5x-0.16-M27|420330-9901-000]]  ​
-|  ​20x  ​| ​ [[https://​www.micro-shop.zeiss.com/​?​s=3407786617d462&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=440341-9904-000|EC Plan-Neofluar ​Ph 2]]  |  ​Dry  ​|  ​0.50  ​|  ​ | +|  ​20x  ​|  ​EC Plan-Neofluar Ph 2  ​|  ​Air  ​| ​ ​0.50  ​|  ​ ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​440341-9904-000/Objective-EC-Plan-Neofluar-20x-0.5-Ph2|440341-9904-000]]  ​
-|  ​40x  ​| ​ [[https://​www.micro-shop.zeiss.com/​?​s=129353259d52f12&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=420660-9970-000|Plan-Apochromat]] ​ |  ​Dry  ​|  ​0.95  ​|  ​0.25  +|  ​40x  ​|  ​Plan-Apochromat Corr  ​|  ​Air  ​| ​ ​0.95  ​|  ​0.25  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420660-9970-000/Objective-Plan-Apochromat-40x-0.95-Corr-M27|420660-9970-000]]  ​
-|  ​40x  ​| ​ [[https://​www.micro-shop.zeiss.com/​?​s=3407786617d462&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=440451-9903-000|Plan-Neofluar Ph3]]  |  Oil  |  1.30  ​| ​ 0.20  | +|  ​40x  ​|  ​Plan-Neofluar Ph3  ​|  ​Oil  |  1.30  ​|  ​0.20  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​440451-9903-000/​Objective-EC-Plan-Neofluar-40x-1.30-Oil-Ph3|440451-9903-000]]  | 
-|  63x  ​| ​ [[https://​www.micro-shop.zeiss.com/​?​s=571858182d6d9d&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=420781-9910-000|Plan-Apochromat Ph3]]  |  Oil  |  1. ​| ​ 0.19  | +|  63x  |  Plan-Apochromat Ph3  ​| ​ Oil  |  1. ​| ​ 0.19  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420781-9910-000/Objective-Plan-Apochromat-63-1.4-Oil-Ph3-M27|420781-9910-000]]  | 
-|  100x  ​| ​ [[https://​www.micro-shop.zeiss.com/​?​s=3407786617d462&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=440782-9902-000|Plan-Apochromat]] ​ |  ​Oil  ​|  ​1.40  ​| ​ 0.17  |+|  100x  |  Plan-Apochromat DIC  ​| ​ Oil  |  1.40  ​| ​ 0.17  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​440782-9902-000/Objective-Plan-Apochromat-100x-1.40-Oil-DIC|440782-9902-000]]  | 
 + 
 +==== Camera ==== 
 + Model  ^  Type  ^  Frame Size  ^  Pixel Size (µm)  ^  Quantum Efficiency ​ ^ 
 +|  ​[[https://​www.zeiss.com/​microscopy/​int/​products/​microscope-cameras/​axiocam-506-mono.html|Axiocam 506 mono CCD]] 
 +  ​|  ​CCD  |  512 x 512  |  16 x 16  |  > 90%  | 
 +|  [[https://​www.hamamatsu.com/​resources/​pdf/​sys/​SCAS0081E_C11440-22CU.pdf|Hamamatsu ORCA-flash4.V2]]  |  sCMOS  |  2048 x 2048  |  6.5 x 6.5  |  82%  |
  
 ===== System Turn On Procedures ===== ===== System Turn On Procedures =====
zeiss_cell_observer.txt · Last modified: 2024/04/01 23:50 by bioimaging