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zeiss_cell_observer [2018/01/10 00:13] bioimaging |
zeiss_cell_observer [2020/01/01 20:11] bioimaging |
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- | <img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_name.jpg"> | + | <img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_name_new.png"> |
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- | <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_axio_observer_wiki.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_axio_observer_wiki.jpg" width=300></a> | + | <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_wiki_new.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_wiki_new.png" width=300></a> |
<td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
- | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_axio_observer_map.jpg">Room P3-A-10</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47331) <br> | + | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_map_new.png">Room P3-A-10</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47331) <br> |
<b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">Carl Zeiss MicroImaging</a><br> | <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">Carl Zeiss MicroImaging</a><br> | ||
<b>Model</b>: Axio Observer<br> | <b>Model</b>: Axio Observer<br> | ||
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<b>Software</b>: ZEN 2 (blue edition)<br> | <b>Software</b>: ZEN 2 (blue edition)<br> | ||
<b>Year</b>: 2015 <br> | <b>Year</b>: 2015 <br> | ||
- | <b>SN</b>: 1026052056<br> | + | <b>SN</b>: 3851002396<br> |
  <br> |   <br> | ||
  <br> |   <br> | ||
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===== Microscope overview ===== | ===== Microscope overview ===== | ||
- | The Zeiss Cell Observer is a fully motorized inverted //widefield fluorescence microscope// ideal for live-cell imaging applications. It is equipped with a large cage incubator and a small stage incubator for temperature control and CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. Using the ZEN 2 software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. Its sensitive cooled Axiocam 506m camera allows you to capture weak fluorescent signals and minimize photobleaching/photodamage in light sensitive samples. You can also perform Z-stack acquisition and use our [[http://micro.magnet.fsu.edu/primer/digitalimaging/deconvolution/deconvolutionhome.html|deconvolution]] server [[brainstorm|Brainstorm]] to significantly improve image resolution even in thick samples. If don't want to use deconvolution, check out one of the confocal systems such as the point scanners [[zeiss_lsm_710|Zeiss LSM 710]] and [[zeiss_lsm_880|Zeiss LSM 880]] or spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]]. If you have FISH or other fixed fluorescence slides try the [[leica_dm5000b|Leica DM5000B]] instead. | + | The Zeiss Cell Observer is a fully motorized inverted //widefield fluorescence microscope// ideal for live-cell imaging applications. It is equipped with a large cage incubator and a small stage incubator for temperature control and CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. Using the ZEN 2 software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. Its sensitive cooled Axiocam 506m camera allows you to capture weak fluorescent signals and minimize photobleaching/photodamage in light sensitive samples. You can also perform Z-stack acquisition and use [[https://huygens.i3s.up.pt/login.php|PPBI Huygens RM]] [[http://micro.magnet.fsu.edu/primer/digitalimaging/deconvolution/deconvolutionhome.html|deconvolution]] server to significantly improve image resolution even in thick samples. If don't want to use deconvolution, check out one of the confocal systems such as the point scanners [[zeiss_lsm_710|Zeiss LSM 710]] and [[zeiss_lsm_880|Zeiss LSM 880]] or spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]]. If you have FISH or other fixed fluorescence slides try the [[leica_dm5000b|Leica DM5000B]] instead. |
* **Microscope**: [[http://www.zeiss.com/microscopy/en_de/products/light-microscopes/axio-observer-for-biology.html|Zeiss Axio Observer]] | * **Microscope**: [[http://www.zeiss.com/microscopy/en_de/products/light-microscopes/axio-observer-for-biology.html|Zeiss Axio Observer]] | ||
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===== Microscope Turn Off procedures ===== | ===== Microscope Turn Off procedures ===== | ||
* Exit the **ZEN 2** software | * Exit the **ZEN 2** software | ||
- | * Turn off the computer | + | * Turn off the computer (not the UPS!) |
+ | {{:cell_observer_pc_vs_ups.png?250|}} | ||
* If you used any __immersion oil in the objectives, clean them up__. | * If you used any __immersion oil in the objectives, clean them up__. | ||
* Turn off the microscope power supply (B) | * Turn off the microscope power supply (B) |