zeiss_cell_observer
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| <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_axio_observer_wiki.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_axio_observer_wiki.jpg" width=300></a> | <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_axio_observer_wiki.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_axio_observer_wiki.jpg" width=300></a> | ||
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| - | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_axio_observer_map.jpg">Room P3-A-10</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47311) <br> | + | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_axio_observer_map.jpg">Room P3-A-10</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47331) <br> |
| <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">Carl Zeiss MicroImaging</a><br> | <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">Carl Zeiss MicroImaging</a><br> | ||
| <b>Model</b>: Axio Observer<br> | <b>Model</b>: Axio Observer<br> | ||
| + | <b>Nickname</b>: "Cell Observer"<br> | ||
| <b>Software</b>: ZEN 2 (blue edition)<br> | <b>Software</b>: ZEN 2 (blue edition)<br> | ||
| <b>Year</b>: 2015 <br> | <b>Year</b>: 2015 <br> | ||
| <b>SN</b>: 1026052056<br> | <b>SN</b>: 1026052056<br> | ||
| - |   <br> | ||
|   <br> |   <br> | ||
|   <br> |   <br> | ||
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| ===== Microscope overview ===== | ===== Microscope overview ===== | ||
| - | The Zeiss Cell Observer is a fully motorized inverted //widefield fluorescence microscope// ideal for live-cell imaging applications. It is equipped with a large cage incubator and a small stage incubator for temperature control and CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. Using the ZEN 2 software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. Its sensitive cooled Axiocam 506m camera allows you to capture weak fluorescent signals and minimize photobleaching/photodamage in light sensitive samples. You can also perform Z-stack acquisition and use our [[http://micro.magnet.fsu.edu/primer/digitalimaging/deconvolution/deconvolutionhome.html|deconvolution]] server [[brainstorm|Brainstorm]] to significantly improve image resolution even in thick samples. If don't want to use deconvolution, check out one of the confocal systems such as the [[zeiss_lsm_710|Zeiss LSM 710]], the [[zeiss_lsm_880|Zeiss LSM 880]] or the [[3i_marianas_sdc|3i Marianas SDC]] spinning disk. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]]. If you have FISH or other fixed fluorescence slides try the [[leica_dm5000b|Leica DM5000B]] instead. | + | The Zeiss Cell Observer is a fully motorized inverted //widefield fluorescence microscope// ideal for live-cell imaging applications. It is equipped with a large cage incubator and a small stage incubator for temperature control and CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. Using the ZEN 2 software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. Its sensitive cooled Axiocam 506m camera allows you to capture weak fluorescent signals and minimize photobleaching/photodamage in light sensitive samples. You can also perform Z-stack acquisition and use our [[http://micro.magnet.fsu.edu/primer/digitalimaging/deconvolution/deconvolutionhome.html|deconvolution]] server [[brainstorm|Brainstorm]] to significantly improve image resolution even in thick samples. If don't want to use deconvolution, check out one of the confocal systems such as the point scanners [[zeiss_lsm_710|Zeiss LSM 710]] and [[zeiss_lsm_880|Zeiss LSM 880]] or spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]]. If you have FISH or other fixed fluorescence slides try the [[leica_dm5000b|Leica DM5000B]] instead. |
| + | |||
| + | * **Microscope**: [[http://www.zeiss.com/microscopy/en_de/products/light-microscopes/axio-observer-for-biology.html|Zeiss Axio Observer]] | ||
| + | * **Camera**: [[https://www.zeiss.com/microscopy/int/products/microscope-cameras/axiocam-506-mono.html|Axiocam 506 mono CCD]] | ||
| + | |||
| + | {{warning.jpg?20|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | ||
| ===== Booking Rules ===== | ===== Booking Rules ===== | ||
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| {{zeiss_axio_observer_b.jpg?height=150|}} | {{zeiss_axio_observer_b.jpg?height=150|}} | ||
| * Turn on the computer | * Turn on the computer | ||
| - | * Log in Windows with your lab user account (each lab has it's own area and login). | + | * Log in Windows with Cell Observer user account. |
| * Start the **ZEN 2** software | * Start the **ZEN 2** software | ||
zeiss_cell_observer.txt · Last modified: 2024/04/01 23:50 by bioimaging
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