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zeiss_cell_observer [2017/04/13 18:30] bioimaging created |
zeiss_cell_observer [2017/12/28 12:32] bioimaging [System Turn On Procedures] |
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<b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">Carl Zeiss MicroImaging</a><br> | <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">Carl Zeiss MicroImaging</a><br> | ||
<b>Model</b>: Axio Observer<br> | <b>Model</b>: Axio Observer<br> | ||
+ | <b>Nickname</b>: "Cell Observer"<br> | ||
<b>Software</b>: ZEN 2 (blue edition)<br> | <b>Software</b>: ZEN 2 (blue edition)<br> | ||
<b>Year</b>: 2015 <br> | <b>Year</b>: 2015 <br> | ||
<b>SN</b>: 1026052056<br> | <b>SN</b>: 1026052056<br> | ||
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===== Microscope overview ===== | ===== Microscope overview ===== | ||
- | The Zeiss Cell Observer is a fully motorized inverted //widefield fluorescence microscope// ideal for live-cell imaging applications. It is equipped with a large cage incubator and a small stage incubator for temperature control and CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. Using the ZEN 2 software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. Its sensitive cooled Axiocam 506m camera allows you to capture weak fluorescent signals and minimize photobleaching/photodamage in light sensitive samples. You can also perform Z-stack acquisition and use our [[http://micro.magnet.fsu.edu/primer/digitalimaging/deconvolution/deconvolutionhome.html|deconvolution]] server [[brainstorm|Brainstorm]] to significantly improve image resolution even in thick samples. If don't want to use deconvolution, check out one of the confocal systems such as the [[zeiss_lsm_710|Zeiss LSM 710]], the [[zeiss_lsm_880|Zeiss LSM 880]] or the [[3i_marianas_sdc|3i Marianas SDC]] spinning disk. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]]. If you have FISH or other fixed fluorescence slides try the [[leica_dm5000b|Leica DM5000B]] instead. | + | The Zeiss Cell Observer is a fully motorized inverted //widefield fluorescence microscope// ideal for live-cell imaging applications. It is equipped with a large cage incubator and a small stage incubator for temperature control and CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. Using the ZEN 2 software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. Its sensitive cooled Axiocam 506m camera allows you to capture weak fluorescent signals and minimize photobleaching/photodamage in light sensitive samples. You can also perform Z-stack acquisition and use our [[http://micro.magnet.fsu.edu/primer/digitalimaging/deconvolution/deconvolutionhome.html|deconvolution]] server [[brainstorm|Brainstorm]] to significantly improve image resolution even in thick samples. If don't want to use deconvolution, check out one of the confocal systems such as the point scanners [[zeiss_lsm_710|Zeiss LSM 710]] and [[zeiss_lsm_880|Zeiss LSM 880]] or spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]]. If you have FISH or other fixed fluorescence slides try the [[leica_dm5000b|Leica DM5000B]] instead. |
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+ | * **Microscope**: [[http://www.zeiss.com/microscopy/en_de/products/light-microscopes/axio-observer-for-biology.html|Zeiss Axio Observer]] | ||
+ | * **Camera**: [[https://www.zeiss.com/microscopy/int/products/microscope-cameras/axiocam-506-mono.html|Axiocam 506 mono CCD]] | ||
+ | |||
+ | {{warning.jpg?20|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | ||
===== Booking Rules ===== | ===== Booking Rules ===== | ||
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{{zeiss_axio_observer_b.jpg?height=150|}} | {{zeiss_axio_observer_b.jpg?height=150|}} | ||
* Turn on the computer | * Turn on the computer | ||
- | * Log in Windows with your lab user account (each lab has it's own area and login). | + | * Log in Windows with Cell Observer user account. |
* Start the **ZEN 2** software | * Start the **ZEN 2** software | ||