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zeiss_axiovert_200m [2020/01/01 22:09] bioimaging [Microscope Turn Off procedures] |
zeiss_axiovert_200m [2020/01/14 17:42] bioimaging [System Turn On Procedures] |
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*** pixel sizes for binning = 1** | *** pixel sizes for binning = 1** | ||
+ | |||
+ | ==== Cameras ==== | ||
+ | ^ Model ^ Type ^ Frame Size ^ Pixel Size (µm) ^ | ||
+ | | [[https://labs.pbrc.edu/cellbiology/documents/CoolSNAPHQ.pdf|Photometrics Coolsnap HQ]] | CCD | 1392 x 1040 | 6.45 x 6.45 | | ||
+ | | [[https://www.leica-microsystems.com/products/microscope-cameras/p/leica-dfc450-c/|Leica DFC450]] | color CCD | 2560 × 1920 | 3.4 x 3.4 | | ||
==== Stage ==== | ==== Stage ==== | ||
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===== System Turn On Procedures ===== | ===== System Turn On Procedures ===== | ||
- | * Turn the fluorescence light power source on. | + | * Turn on the fluorescent light power source |
{{halide_710.jpg?80|}} | {{halide_710.jpg?80|}} | ||
- | * Turn the Prior microscope stage controller on. | + | * Turn on the Prior stage controller |
{{ax_prior_on.jpg?100|}} | {{ax_prior_on.jpg?100|}} | ||
- | * Wait 10s then turn the microscope on. | + | * Turn on the microscope |
{{ax_mic_on.jpg?100|}} | {{ax_mic_on.jpg?100|}} | ||
- | * Turn the camera on. | + | * Turn on the CCD camera |
{{ax_cam_on.jpg?100|}} | {{ax_cam_on.jpg?100|}} | ||
- | * Turn the computer on. | + | * Turn on the computer |
- | * Login to the computer (Bioimaging User). | + | * Log in to Windows (Bioimaging User) |
- | * Start the Metamorph software. | + | * Start the Metamorph software |
+ | * If the software doesn't detect the camera, power cycle the camera and try again | ||
===== Microscope Turn Off procedures ===== | ===== Microscope Turn Off procedures ===== | ||
* Turn off the computer. | * Turn off the computer. |