zeiss_axiovert_200m
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zeiss_axiovert_200m [2020/01/01 22:07] bioimaging [Objectives] |
zeiss_axiovert_200m [2020/01/01 22:13] bioimaging |
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| *** pixel sizes for binning = 1** | *** pixel sizes for binning = 1** | ||
| + | |||
| + | ==== Cameras ==== | ||
| + | ^ Model ^ Type ^ Frame Size ^ Pixel Size (µm) ^ | ||
| + | | [[https://labs.pbrc.edu/cellbiology/documents/CoolSNAPHQ.pdf|Photometrics Coolsnap HQ]] | CCD | 1392 x 1040 | 6.45 x 6.45 | | ||
| + | | [[https://www.leica-microsystems.com/products/microscope-cameras/p/leica-dfc450-c/|Leica DFC450]] | color CCD | 2560 × 1920 | 3.4 x 3.4 | | ||
| ==== Stage ==== | ==== Stage ==== | ||
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| * Turn the fluorescence light power source on. | * Turn the fluorescence light power source on. | ||
| {{halide_710.jpg?80|}} | {{halide_710.jpg?80|}} | ||
| - | * Turn on the Uniblitz shutter controller (on the back). | ||
| - | {{ax_uniblitz1.jpg?100|}}{{ax_uniblitz2.jpg?100|}} | ||
| * Turn the Prior microscope stage controller on. | * Turn the Prior microscope stage controller on. | ||
| {{ax_prior_on.jpg?100|}} | {{ax_prior_on.jpg?100|}} | ||
| Line 98: | Line 101: | ||
| {{ax_cam_on.jpg?100|}} | {{ax_cam_on.jpg?100|}} | ||
| * Turn the computer on. | * Turn the computer on. | ||
| - | * Login to the computer (Axiovert 200M user). | + | * Login to the computer (Bioimaging User). |
| * Start the Metamorph software. | * Start the Metamorph software. | ||
| - | * If you need temperature control, turn on the control box | ||
| - | {{heating2.jpg?100|}} | ||
| - | * If you need CO2, open the valve and turn on CO2 supply on the control box | ||
| - | {{co2.jpg?100|}} | ||
| - | |||
| ===== Microscope Turn Off procedures ===== | ===== Microscope Turn Off procedures ===== | ||
| * Turn off the computer. | * Turn off the computer. | ||
| Line 110: | Line 108: | ||
| * If you used any __immersion oil in the objectives, clean them up__. | * If you used any __immersion oil in the objectives, clean them up__. | ||
| * Turn off the microscope stage controller. | * Turn off the microscope stage controller. | ||
| - | * Turn off the shutter controller. | ||
| * Turn off the microscope. | * Turn off the microscope. | ||
| * If there's __another user__ for this microscope in the __next hour__ leave the mercury __lamp power source on, else turn it off__. | * If there's __another user__ for this microscope in the __next hour__ leave the mercury __lamp power source on, else turn it off__. | ||
zeiss_axiovert_200m.txt · Last modified: 2024/02/12 22:50 by bioimaging
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