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zeiss_axiovert_200m [2016/06/08 17:31] bioimaging [Objectives] |
zeiss_axiovert_200m [2020/01/01 20:39] bioimaging |
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- | <img src="/facility/bioimaging/lib/exe/fetch.php?media=axiovert_200m_name.jpg"> | + | <img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_axiovert_200m_name_new.png"> |
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- | <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_axiovert_200m_wiki.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_axiovert_200m_wiki.jpg" width=300></a> | + | <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_axiovert_200m_wiki_new.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_axiovert_200m_wiki_new.png" width=300></a> |
<td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
- | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_axiovert_200m_map.jpg">Room P2-A-22</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47217) <br> | + | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_axiovert_200m_map_new.png">Room P2-A-22</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47217) <br> |
<b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">Carl Zeiss MicroImaging</a><br> | <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">Carl Zeiss MicroImaging</a><br> | ||
<b>Model</b>: Axiovert 200M<br> | <b>Model</b>: Axiovert 200M<br> | ||
+ | <b>Nickname</b>: "Axiovert"<br> | ||
<b>Software</b>: Metamorph 7.7.9.0<br> | <b>Software</b>: Metamorph 7.7.9.0<br> | ||
<b>Year</b>: 2003 <br> | <b>Year</b>: 2003 <br> | ||
<b>SN</b>: ZCA522000494<br> | <b>SN</b>: ZCA522000494<br> | ||
- |   <br> | ||
  <br> |   <br> | ||
  <br> |   <br> | ||
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===== Microscope overview ===== | ===== Microscope overview ===== | ||
- | The Zeiss Axiovert 200M is a fully motorized inverted //widefield fluorescence microscope// ideal for live-cell imaging applications. It is equipped with a small stage incubator for temperature control and CO2 supply. Using the Metamorph software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. Its sensitive cooled CCD camera (Roper Scientific Coolsnap HQ CCD) allows you to capture weak fluorescent signals and minimize photobleaching/photodamage in light sensitive samples. You can also perform Z-stack acquisition and use our [[http://micro.magnet.fsu.edu/primer/digitalimaging/deconvolution/deconvolutionhome.html|deconvolution]] server [[brainstorm|Brainstorm]] to significantly improve image resolution even in thick samples. If you are performing long time-lapse experiments in live samples (aqueous media) and need hardware focus control, check the [[zeiss_cell_observer_wf|Zeiss Cell Observer WF]] with Definite Focus. If don't want to use deconvolution, check out one of the confocal systems such as the [[zeiss_lsm_710|Zeiss LSM 710]], [[zeiss_lsm_880|Zeiss LSM 880]] or the [[3i_marianas_sdc|3i Marianas SDC]] spinning disk. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]]. If you have FISH or other fixed fluorescence slides try the [[leica_dm5000b|Leica DM5000B]] instead. | + | The Zeiss Axiovert 200M is a fully motorized inverted //widefield fluorescence microscope// ideal for live-cell imaging applications. It is equipped with a small stage incubator for temperature control and CO2 supply. Using the Metamorph software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. Its sensitive cooled CCD camera (Photometrics Coolsnap HQ CCD) allows you to capture weak fluorescent signals and minimize photobleaching/photodamage in light sensitive samples. You can also perform Z-stack acquisition and use our [[http://micro.magnet.fsu.edu/primer/digitalimaging/deconvolution/deconvolutionhome.html|deconvolution]] server [[brainstorm|Brainstorm]] to significantly improve image resolution even in thick samples. If you are performing long time-lapse experiments in live samples (aqueous media) and need hardware focus control, check the [[zeiss_cell_observer|Zeiss Cell Observer]] with Definite Focus. If don't want to use deconvolution, check out one of the confocal systems such as the point scanners [[zeiss_lsm_710|Zeiss LSM 710]] and [[zeiss_lsm_880|Zeiss LSM 880]] or the spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]]. If you have FISH or other fixed fluorescence slides try the [[leica_dm5000b|Leica DM5000B]] instead. |
+ | |||
+ | * **Microscope**: Zeiss Axiovert 200 | ||
+ | * **Camera**: Photometrics Coolsnap HQ CCD | ||
+ | |||
+ | {{warning.jpg?20|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | ||
===== Booking Rules ===== | ===== Booking Rules ===== | ||
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- The above rules do not apply for sessions booked on the very same day or **24 hours ahead** | - The above rules do not apply for sessions booked on the very same day or **24 hours ahead** | ||
- Exceptions to these rules require **approval from [[joserino@medicina.ulisboa.pt|José Rino]]**. | - Exceptions to these rules require **approval from [[joserino@medicina.ulisboa.pt|José Rino]]**. | ||
- | - Users must indicate which lasers they are going to use in the booking Description field | ||
- | - Users must indicate if they will use the incubator in the booking Description field (write down 37C INCUBATOR) | ||
- | - Users must write their internal extension phone number in the booking Description field | ||
===== System components ===== | ===== System components ===== | ||
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| 5x | [[https://www.micro-shop.zeiss.com/?s=571974962233f6&l=en&p=uk&f=o&a=v&m=s&id=420330-9901-000|EC Plan-NeoFluar]] | Dry | 0.16 | 18.5 | 2.00 | 5 pixels | | | 5x | [[https://www.micro-shop.zeiss.com/?s=571974962233f6&l=en&p=uk&f=o&a=v&m=s&id=420330-9901-000|EC Plan-NeoFluar]] | Dry | 0.16 | 18.5 | 2.00 | 5 pixels | | ||
| 20x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=440341-9904-000|EC Plan-Neofluar Ph 2]] | Dry | 0.50 | 2 | 0.48 | 21 pixels | | | 20x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=440341-9904-000|EC Plan-Neofluar Ph 2]] | Dry | 0.50 | 2 | 0.48 | 21 pixels | | ||
- | | 63x (Ph 3) | [[https://www.micro-shop.zeiss.com/?s=571858182d6d9d&l=en&p=uk&f=o&a=v&m=s&id=420781-9910-000|Plan-Apochromat]] | Oil | 1.4 | 0.19 | 0.15 | 65 pixels | | + | | 40x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=440451-9903-000|Plan-Neofluar Ph3]] | Oil | 1.30 | 0.20 | 0.24 | 41 pixels | |
- | | 100x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=440782-9902-000|Plan-Apochromat]] | Oil | 1.40 | 0.17 | 0.10 | 102 pixels | - | | + | | 63x | [[https://www.micro-shop.zeiss.com/?s=571858182d6d9d&l=en&p=uk&f=o&a=v&m=s&id=420781-9910-000|Plan-Apochromat Ph3]] | Oil | 1.4 | 0.19 | 0.15 | 65 pixels | |
*** pixel sizes for binning = 1** | *** pixel sizes for binning = 1** | ||
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{{ax_cam_on.jpg?100|}} | {{ax_cam_on.jpg?100|}} | ||
* Turn the computer on. | * Turn the computer on. | ||
- | * Login to the computer (each group has it's own area and login). | + | * Login to the computer (Axiovert 200M user). |
* Start the Metamorph software. | * Start the Metamorph software. | ||
* If you need temperature control, turn on the control box | * If you need temperature control, turn on the control box |