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- | ====== Microscope Quality Control ====== | + | {{:quality_control_neg.png|}} |
- | ==== PSFs ==== | + | {{ ::psfj_fit.png?150|}} |
+ | {{::imm_question.jpg?140 |Image by Maria Henriques, João Ferreira and José Rino}} **PSFs** \\ | ||
+ | We image sub-resolution fluorescent beads (0.17 μm diameter) and use [[http://www.knoplab.de/psfj|PSFj]] to analyze the acquired Point Spread Functions (PSFs) and determine the resolution in x, y and z for each objective.\\ | ||
+ | \\ | ||
+ | **Illumination**\\ | ||
+ | We measure the intensity of laser lines, LEDs and fluorescent lamps for each system. | ||
+ | \\ | ||
+ | \\ | ||
+ | \\ | ||
- | {{psf.gif?100 |}} We image sub-resolution fluorescent beads (0.17 μm diameter) and use [[http://imagejdocu.tudor.lu/doku.php?id=plugin:analysis:metroloj:start|MetroloJ]] to analyze the acquired Point Spread Functions (PSFs) and determine the resolution in x, y and z for each objective. | + | === Spinning Disc Confocal Microscopes === |
- | ==== Illumination ==== | + | [[3i_marianas_sdc_quality|{{::3i_marianas_sdc_with_name.png?160|}}]] [[zeiss_cell_observer_sd_quality|{{::zeiss_cell_observer_sd_with_name.png?160|}}]] |
- | We measure the intensity of fluorescent lamps and laser lines in each system. | + | === Point Scanning Confocal Microscopes === |
- | ==== Check the Systems ==== | + | [[zeiss_lsm_710_quality|{{::zeiss_lsm_710_with_name.png?160|}}]] [[zeiss_lsm_880_quality|{{::zeiss_lsm_880_with_name.png?160|}}]] |
- | === High Speed Confocal Microscopes === | + | === Multi-photon Microscope === |
- | * [[3i_Marianas SDC_quality|3i Marianas SDC]] | + | |
- | * [[zeiss_lsm_7_live_quality|Zeiss LSM 7 Live]] | + | |
- | === Point Scanning Confocal Microscopes === | + | [[leica_sp8_mp_quality|{{::leica_sp8_mp_with_name.png?160|}}]] |
- | * [[zeiss_lsm_880_quality|Zeiss LSM 880]] | + | |
- | * [[zeiss_lsm_710_quality|Zeiss LSM 710]] | + | === Light Sheet Fluorescence Microscope === |
+ | |||
+ | [[zeiss_lightsheet_z1_quality|{{::zeiss_lightsheet_z1_with_name.png?160|}}]] | ||
=== Widefield Fluorescence Microscopes === | === Widefield Fluorescence Microscopes === | ||
- | * [[zeiss_axiovert200m_quality|Zeiss Axiovert 200M]] | + | |
- | * [[zeiss_cell_observer_wf_quality|Zeiss Cell Observer WF]] | + | [[zeiss_cell_observer_quality|{{::zeiss_cell_observer_with_name.png?160|}}]] [[zeiss_axiovert_200m_quality|{{::zeiss_axiovert_200m_with_name.png?160|}}]] [[nikon_eclipse_ti_quality|{{::nikon_eclipse_ti_with_name.png?160|}}]] [[leica_dm5000b_quality|{{::leica_dm5000b_with_name.png?160|}}]] |
+ | |||
+ | === Fluorescence Stereo Microscope === | ||
+ | |||
+ | [[zeiss_axiozoom_v16_quality|{{::zeiss_axiozoom_v16_with_name.png?160|}}]] | ||
+ | |||
+ | ==== Other Imaging Systems ==== | ||
+ | |||
+ | [[ivis_lumina_quality|{{::ivis_lumina_with_name.png?160|}}]] | ||
---- | ---- | ||
=== No longer available... === | === No longer available... === | ||
- | * [[Zeiss_LSM 510 META_quality|LSM 510 META (retired)]] | + | * [[zeiss_lsm_7_live_quality|Zeiss LSM 7 Live]] (retired) |
+ | * [[Zeiss_LSM 510 META_quality|LSM 510 META]] (retired) | ||
---- | ---- | ||
- | [[resources|Back to the Resources page]] | + | [[start|Back to the Bioimaging Wiki]] |