nikon_eclipse_ti
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nikon_eclipse_ti [2023/02/22 02:04] bioimaging [Filtersets] |
nikon_eclipse_ti [2024/01/27 23:52] bioimaging [Microscope overview] |
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| <b>SN</b>: 602805 <br> | <b>SN</b>: 602805 <br> | ||
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| + | Data will be deleted after: <b>3 months</b> | ||
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| ===== Microscope overview ===== | ===== Microscope overview ===== | ||
| - | {{ :widefield_lightpath.png?0x300}}The Nikon Eclipse Ti is a fully motorized inverted //widefield fluorescence microscope// ideal for live-cell imaging applications. It is equipped with a small stage incubator for temperature control and CO2 supply and with Perfect Focus System for hardware focus control during long time-lapse acquisitions. Using either Metamorph or Nikon Elements software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. It is equipped with a Andor Neo 5.5 monochrome cooled sCMOS camera ideal for time-lapse and tile-and-stitch microscopy. You can perform Z-stack acquisition and use [[https://huygens.i3s.up.pt/login.php|PPBI Huygens RM]] [[http://micro.magnet.fsu.edu/primer/digitalimaging/deconvolution/deconvolutionhome.html|deconvolution]] server to significantly improve image resolution even in thick samples. If don't want to use deconvolution, check out one of the confocal systems such as the point scanners [[zeiss_lsm_710|Zeiss LSM 710]] and [[zeiss_lsm_880|Zeiss LSM 880]] or spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]. If your personal computer cannot handle all the data you collected, check out the [[colossus|Colossus]]. | + | {{ :widefield_lightpath.png?0x300}}The Nikon Eclipse Ti is a fully motorized inverted //widefield fluorescence microscope// ideal for live-cell imaging applications. It is equipped with a small stage incubator for temperature control and CO2 supply and with Perfect Focus System for hardware focus control during long time-lapse acquisitions. Using NIS-Elements software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. It is equipped with a Andor Neo 5.5 monochrome cooled sCMOS camera ideal for time-lapse and tile-and-stitch microscopy. You can perform Z-stack acquisition and use [[https://huygens.i3s.up.pt/login.php|PPBI Huygens RM]] [[http://micro.magnet.fsu.edu/primer/digitalimaging/deconvolution/deconvolutionhome.html|deconvolution]] server to significantly improve image resolution even in thick samples. If don't want to use deconvolution, check out one of the confocal systems such as the point scanners [[zeiss_lsm_710|Zeiss LSM 710]] and [[zeiss_lsm_880|Zeiss LSM 880]] or spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]. If your personal computer cannot handle all the data you collected, check out the [[colossus|Colossus]]. |
| * **Microscope**: [[https://www.microscope.healthcare.nikon.com/en_EU/products/inverted-microscopes/eclipse-ti-series|Nikon Eclipse Ti]] | * **Microscope**: [[https://www.microscope.healthcare.nikon.com/en_EU/products/inverted-microscopes/eclipse-ti-series|Nikon Eclipse Ti]] | ||
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| ==== Filtersets Turret 2 (bottom) ==== | ==== Filtersets Turret 2 (bottom) ==== | ||
| - | ^ Position ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ | + | ^ Position ^ Filterset ^ |
| - | | 1 | Empty ||||| | + | | 1 | Empty | |
| - | | 2 | Empty ||||| | + | | 2 | Empty | |
| - | | 3 | Empty ||||| | + | | 3 | Empty | |
| - | | 4 | Empty ||||| | + | | 4 | Empty | |
| - | | 5 | Empty ||||| | + | | 5 | Empty | |
| - | | 6 | Fura-2 ||||| | + | | 6 | Fura-2 | |
| ==== Objectives ==== | ==== Objectives ==== | ||
nikon_eclipse_ti.txt · Last modified: 2024/02/12 22:47 by bioimaging
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