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nikon_eclipse_ti [2021/03/27 00:56] bioimaging [Camera] |
nikon_eclipse_ti [2023/03/27 13:09] bioimaging |
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- | <a href="/facility/bioimaging/lib/exe/fetch.php?media=nikon_eclipse_ti_wiki_new.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=nikon_eclipse_ti_wiki_new.png" width=300></a> | + | <a href="/facility/bioimaging/lib/exe/fetch.php?media=nikon_eclipse_ti_wiki_2022.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=nikon_eclipse_ti_wiki_2022.png" width=300></a> |
<td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
- | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=nikon_eclipse_ti_map_new.png">Room P3-A-5</a> <br> | + | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=nikon_eclipse_ti_map_new_p2a24.png">Room P2-A-24</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47219) <br> |
<b>Manufacturer</b>: <a href="https://www.microscope.healthcare.nikon.com/en_EU/" target="_blank">Nikon Instruments</a><br> | <b>Manufacturer</b>: <a href="https://www.microscope.healthcare.nikon.com/en_EU/" target="_blank">Nikon Instruments</a><br> | ||
<b>Model</b>: Eclipse Ti<br> | <b>Model</b>: Eclipse Ti<br> | ||
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<b>SN</b>: 602805 <br> | <b>SN</b>: 602805 <br> | ||
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+ | Data will be deleted after: <b>3 months</b> | ||
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- | → <a href="https://my.agendo.science/calendar/?id=MzhjYWxlbmRhcnM="><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> Nikon Eclipse Ti Booking</a> | + | → <a href="/facility/bioimaging/doku.php?id=nikon_eclipse_ti_quality"><img src="/facility/bioimaging/lib/exe/fetch.php?media=list-icon.png" width=18> Nikon Eclipse Ti Quality Control</a> |
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→ <a href="/facility/bioimaging/doku.php?id=nikon_eclipse_ti_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> Nikon Eclipse Ti Usage Statistics</a> | → <a href="/facility/bioimaging/doku.php?id=nikon_eclipse_ti_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> Nikon Eclipse Ti Usage Statistics</a> | ||
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===== Microscope overview ===== | ===== Microscope overview ===== | ||
- | {{ :widefield_lightpath.png?0x300}}The Nikon Eclipse Ti is a fully motorized inverted //widefield fluorescence microscope// ideal for live-cell imaging applications. It is equipped with a small stage incubator for temperature control and CO2 supply and with Perfect Focus System for hardware focus control during long time-lapse acquisitions. Using either Metamorph or Nikon Elements software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. It is equipped with a Retiga R6 monochrome CCD camera that allows for a large field of view, ideal for time-lapse and tile-and-stitch microscopy. You can perform Z-stack acquisition and use [[https://huygens.i3s.up.pt/login.php|PPBI Huygens RM]] [[http://micro.magnet.fsu.edu/primer/digitalimaging/deconvolution/deconvolutionhome.html|deconvolution]] server to significantly improve image resolution even in thick samples. If don't want to use deconvolution, check out one of the confocal systems such as the point scanners [[zeiss_lsm_710|Zeiss LSM 710]] and [[zeiss_lsm_880|Zeiss LSM 880]] or spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]]. | + | {{ :widefield_lightpath.png?0x300}}The Nikon Eclipse Ti is a fully motorized inverted //widefield fluorescence microscope// ideal for live-cell imaging applications. It is equipped with a small stage incubator for temperature control and CO2 supply and with Perfect Focus System for hardware focus control during long time-lapse acquisitions. Using either Metamorph or Nikon Elements software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. It is equipped with a Andor Neo 5.5 monochrome cooled sCMOS camera ideal for time-lapse and tile-and-stitch microscopy. You can perform Z-stack acquisition and use [[https://huygens.i3s.up.pt/login.php|PPBI Huygens RM]] [[http://micro.magnet.fsu.edu/primer/digitalimaging/deconvolution/deconvolutionhome.html|deconvolution]] server to significantly improve image resolution even in thick samples. If don't want to use deconvolution, check out one of the confocal systems such as the point scanners [[zeiss_lsm_710|Zeiss LSM 710]] and [[zeiss_lsm_880|Zeiss LSM 880]] or spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]. If your personal computer cannot handle all the data you collected, check out the [[colossus|Colossus]]. |
* **Microscope**: [[https://www.microscope.healthcare.nikon.com/en_EU/products/inverted-microscopes/eclipse-ti-series|Nikon Eclipse Ti]] | * **Microscope**: [[https://www.microscope.healthcare.nikon.com/en_EU/products/inverted-microscopes/eclipse-ti-series|Nikon Eclipse Ti]] | ||
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===== System components ===== | ===== System components ===== | ||
- | ==== Filtersets ==== | + | ==== Filtersets Turret 1 (top) ==== |
^ Position ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ | ^ Position ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ | ||
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| 6 | Empty ||||| | | 6 | Empty ||||| | ||
- | ==== Objectives ==== | + | ==== Filtersets Turret 2 (bottom) ==== |
- | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ pixel size* (μm) ^ 10 μm = ^ Reference ^ | + | |
- | | 10x | CFI Plan Fluor DLL Ph1 | Air | 0.30 | 16.00 | 0.454 | 22 pixels | [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1835|MRH10101]] | | + | |
- | | 20x | CFI S Plan Fluor ELWD ADM Ph1 | Air | 0.45 | 6.90 - 8.20 | 0.227 | 44 pixels | [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1813|MRH48230]] | | + | |
- | | 40x | CFI S Plan Fluor ELWD ADM Ph2 | Air | 0.60 | 2.80 - 3.60 | 0.115 | 86 pixels | [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1814|MRH48430]] | | + | |
- | | 40x | Plan Apo DM Ph3 | Oil | 1.00 | 0.16 | 0.113 | 88 pixels | | | + | |
- | **Upon request:** | + | ^ Position ^ Filterset ^ |
- | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ pixel size* (μm) ^ 10 μm = ^ Reference ^ | + | | 1 | Empty | |
- | | 40x | Plan Fluor ELWD ADL Ph2 | Air | 0.60 | 2.70 - 3.70 | 0.113 | 88 pixels | | | + | | 2 | Empty | |
+ | | 3 | Empty | | ||
+ | | 4 | Empty | | ||
+ | | 5 | Empty | | ||
+ | | 6 | Fura-2 | | ||
+ | ==== Objectives ==== | ||
+ | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ||
+ | | 10x | CFI Plan Fluor DLL Ph1 | Air | 0.30 | 16.00 | [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1835|MRH10101]] | | ||
+ | | 20x | CFI S Plan Fluor ELWD ADM Ph1 | Air | 0.45 | 6.90 - 8.20 | [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1813|MRH48230]] | | ||
+ | | 40x | CFI CFI Plan Apochromat Lambda D | Air | 0.95 | 0.21 | [[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-179806|MRH70470]] | | ||
+ | | 40x | CFI S Plan Fluor ELWD ADM Ph2 | Air | 0.60 | 2.80 - 3.60 | [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1814|MRH48430]] | | ||
+ | | 60x | Plan Apo DM Ph3 | Oil | 1.40 | 0.13 | | | ||
+ | | 100x | Plan Apo DM Ph3 | Oil | 1.40 | 0.13 | | | ||
- | *** pixel sizes for binning = 1** | + | **Upon request:** |
+ | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ||
+ | | 100x | CFI Plan | Water | 1.10 | 2.50 | [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1956|MRL07920]] | | ||
==== Camera ==== | ==== Camera ==== | ||
^ Model ^ Type ^ Frame Size ^ Pixel Size (µm) ^ | ^ Model ^ Type ^ Frame Size ^ Pixel Size (µm) ^ |